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Microbial Diversity and Pathogenic Properties of Microbiota Associated with Aerobic Vaginitis in Women with Recurrent Pregnancy Loss

Recurrent pregnancy loss (RPL) is a major reproductive problem that affects approximately 5% of couples. The objective of this study was to assess vaginal flora dysbiosis in women suffering from unexplained RPL and to investigate the pathogenic properties of the microbiota associated with aerobic va...

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Autores principales: Ncib, Karima, Bahia, Wael, Leban, Nadia, Mahdhi, Abdelkarim, Trifa, Fatma, Mzoughi, Ridha, Haddad, Anis, Jabeur, Chédia, Donders, Gilbert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600244/
https://www.ncbi.nlm.nih.gov/pubmed/36292132
http://dx.doi.org/10.3390/diagnostics12102444
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author Ncib, Karima
Bahia, Wael
Leban, Nadia
Mahdhi, Abdelkarim
Trifa, Fatma
Mzoughi, Ridha
Haddad, Anis
Jabeur, Chédia
Donders, Gilbert
author_facet Ncib, Karima
Bahia, Wael
Leban, Nadia
Mahdhi, Abdelkarim
Trifa, Fatma
Mzoughi, Ridha
Haddad, Anis
Jabeur, Chédia
Donders, Gilbert
author_sort Ncib, Karima
collection PubMed
description Recurrent pregnancy loss (RPL) is a major reproductive problem that affects approximately 5% of couples. The objective of this study was to assess vaginal flora dysbiosis in women suffering from unexplained RPL and to investigate the pathogenic properties of the microbiota associated with aerobic vaginitis (AV). The study included one hundred fifteen women, 65 with RPL and 50 controls. The diversity of vaginal microbiota isolated was evaluated by molecular sequencing. Then, pathogenic factors, such as acid-resistance, antibiotics susceptibility, and biofilm formation were evaluated. The prevalence of AV was five-fold higher in the RPL group than in the controls (64.6% vs. 12.0%). The most prevalent isolates in the case group were Enterococcus spp. (52%) and Staphylococcus spp. (26%). All bacterial strains tolerate low pH. The prevalence of multidrug resistance (MDR) among all bacteria was 47.7%. Of all strains, 91.0% were biofilm producers. The presence of MDR was found to be related to biofilm formation. The results provide evidence supporting an increased presence of dysbiosis of the vaginal flora, especially AV, in women with RPL in Tunisia. The viability of the AV-associated bacteria and their persistence in the genitals may be due to their ability to resist low pH and to produce a biofilm.
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spelling pubmed-96002442022-10-27 Microbial Diversity and Pathogenic Properties of Microbiota Associated with Aerobic Vaginitis in Women with Recurrent Pregnancy Loss Ncib, Karima Bahia, Wael Leban, Nadia Mahdhi, Abdelkarim Trifa, Fatma Mzoughi, Ridha Haddad, Anis Jabeur, Chédia Donders, Gilbert Diagnostics (Basel) Article Recurrent pregnancy loss (RPL) is a major reproductive problem that affects approximately 5% of couples. The objective of this study was to assess vaginal flora dysbiosis in women suffering from unexplained RPL and to investigate the pathogenic properties of the microbiota associated with aerobic vaginitis (AV). The study included one hundred fifteen women, 65 with RPL and 50 controls. The diversity of vaginal microbiota isolated was evaluated by molecular sequencing. Then, pathogenic factors, such as acid-resistance, antibiotics susceptibility, and biofilm formation were evaluated. The prevalence of AV was five-fold higher in the RPL group than in the controls (64.6% vs. 12.0%). The most prevalent isolates in the case group were Enterococcus spp. (52%) and Staphylococcus spp. (26%). All bacterial strains tolerate low pH. The prevalence of multidrug resistance (MDR) among all bacteria was 47.7%. Of all strains, 91.0% were biofilm producers. The presence of MDR was found to be related to biofilm formation. The results provide evidence supporting an increased presence of dysbiosis of the vaginal flora, especially AV, in women with RPL in Tunisia. The viability of the AV-associated bacteria and their persistence in the genitals may be due to their ability to resist low pH and to produce a biofilm. MDPI 2022-10-09 /pmc/articles/PMC9600244/ /pubmed/36292132 http://dx.doi.org/10.3390/diagnostics12102444 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ncib, Karima
Bahia, Wael
Leban, Nadia
Mahdhi, Abdelkarim
Trifa, Fatma
Mzoughi, Ridha
Haddad, Anis
Jabeur, Chédia
Donders, Gilbert
Microbial Diversity and Pathogenic Properties of Microbiota Associated with Aerobic Vaginitis in Women with Recurrent Pregnancy Loss
title Microbial Diversity and Pathogenic Properties of Microbiota Associated with Aerobic Vaginitis in Women with Recurrent Pregnancy Loss
title_full Microbial Diversity and Pathogenic Properties of Microbiota Associated with Aerobic Vaginitis in Women with Recurrent Pregnancy Loss
title_fullStr Microbial Diversity and Pathogenic Properties of Microbiota Associated with Aerobic Vaginitis in Women with Recurrent Pregnancy Loss
title_full_unstemmed Microbial Diversity and Pathogenic Properties of Microbiota Associated with Aerobic Vaginitis in Women with Recurrent Pregnancy Loss
title_short Microbial Diversity and Pathogenic Properties of Microbiota Associated with Aerobic Vaginitis in Women with Recurrent Pregnancy Loss
title_sort microbial diversity and pathogenic properties of microbiota associated with aerobic vaginitis in women with recurrent pregnancy loss
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600244/
https://www.ncbi.nlm.nih.gov/pubmed/36292132
http://dx.doi.org/10.3390/diagnostics12102444
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