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Approaches towards Elucidating the Metabolic Program of Hematopoietic Stem/Progenitor Cells

Hematopoietic stem cells (HSCs) in bone marrow continuously supply a large number of blood cells throughout life in collaboration with hematopoietic progenitor cells (HPCs). HSCs and HPCs are thought to regulate and utilize intracellular metabolic programs to obtain metabolites, such as adenosine tr...

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Detalles Bibliográficos
Autores principales: Kobayashi, Hiroshi, Watanuki, Shintaro, Takubo, Keiyo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600258/
https://www.ncbi.nlm.nih.gov/pubmed/36291056
http://dx.doi.org/10.3390/cells11203189
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author Kobayashi, Hiroshi
Watanuki, Shintaro
Takubo, Keiyo
author_facet Kobayashi, Hiroshi
Watanuki, Shintaro
Takubo, Keiyo
author_sort Kobayashi, Hiroshi
collection PubMed
description Hematopoietic stem cells (HSCs) in bone marrow continuously supply a large number of blood cells throughout life in collaboration with hematopoietic progenitor cells (HPCs). HSCs and HPCs are thought to regulate and utilize intracellular metabolic programs to obtain metabolites, such as adenosine triphosphate (ATP), which is necessary for various cellular functions. Metabolites not only provide stem/progenitor cells with nutrients for ATP and building block generation but are also utilized for protein modification and epigenetic regulation to maintain cellular characteristics. In recent years, the metabolic programs of tissue stem/progenitor cells and their underlying molecular mechanisms have been elucidated using a variety of metabolic analysis methods. In this review, we first present the advantages and disadvantages of the current approaches applicable to the metabolic analysis of tissue stem/progenitor cells, including HSCs and HPCs. In the second half, we discuss the characteristics and regulatory mechanisms of HSC metabolism, including the decoupling of ATP production by glycolysis and mitochondria. These technologies and findings have the potential to advance stem cell biology and engineering from a metabolic perspective and to establish therapeutic approaches.
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spelling pubmed-96002582022-10-27 Approaches towards Elucidating the Metabolic Program of Hematopoietic Stem/Progenitor Cells Kobayashi, Hiroshi Watanuki, Shintaro Takubo, Keiyo Cells Review Hematopoietic stem cells (HSCs) in bone marrow continuously supply a large number of blood cells throughout life in collaboration with hematopoietic progenitor cells (HPCs). HSCs and HPCs are thought to regulate and utilize intracellular metabolic programs to obtain metabolites, such as adenosine triphosphate (ATP), which is necessary for various cellular functions. Metabolites not only provide stem/progenitor cells with nutrients for ATP and building block generation but are also utilized for protein modification and epigenetic regulation to maintain cellular characteristics. In recent years, the metabolic programs of tissue stem/progenitor cells and their underlying molecular mechanisms have been elucidated using a variety of metabolic analysis methods. In this review, we first present the advantages and disadvantages of the current approaches applicable to the metabolic analysis of tissue stem/progenitor cells, including HSCs and HPCs. In the second half, we discuss the characteristics and regulatory mechanisms of HSC metabolism, including the decoupling of ATP production by glycolysis and mitochondria. These technologies and findings have the potential to advance stem cell biology and engineering from a metabolic perspective and to establish therapeutic approaches. MDPI 2022-10-11 /pmc/articles/PMC9600258/ /pubmed/36291056 http://dx.doi.org/10.3390/cells11203189 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Kobayashi, Hiroshi
Watanuki, Shintaro
Takubo, Keiyo
Approaches towards Elucidating the Metabolic Program of Hematopoietic Stem/Progenitor Cells
title Approaches towards Elucidating the Metabolic Program of Hematopoietic Stem/Progenitor Cells
title_full Approaches towards Elucidating the Metabolic Program of Hematopoietic Stem/Progenitor Cells
title_fullStr Approaches towards Elucidating the Metabolic Program of Hematopoietic Stem/Progenitor Cells
title_full_unstemmed Approaches towards Elucidating the Metabolic Program of Hematopoietic Stem/Progenitor Cells
title_short Approaches towards Elucidating the Metabolic Program of Hematopoietic Stem/Progenitor Cells
title_sort approaches towards elucidating the metabolic program of hematopoietic stem/progenitor cells
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600258/
https://www.ncbi.nlm.nih.gov/pubmed/36291056
http://dx.doi.org/10.3390/cells11203189
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