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Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice

The visualization of choroidal vasculature and innate immune cells in the eyes of pigmented mice has been challenging due to the presence of a retinal pigment epithelium (RPE) layer separating the choroid and retina. Here, we established methods for visualizing the choroidal macrophages, mast cells,...

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Autores principales: Zaitoun, Ismail S., Song, Yong-Seok, Zaitoun, Hammam B., Sorenson, Christine M., Sheibani, Nader
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600292/
https://www.ncbi.nlm.nih.gov/pubmed/36291198
http://dx.doi.org/10.3390/cells11203329
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author Zaitoun, Ismail S.
Song, Yong-Seok
Zaitoun, Hammam B.
Sorenson, Christine M.
Sheibani, Nader
author_facet Zaitoun, Ismail S.
Song, Yong-Seok
Zaitoun, Hammam B.
Sorenson, Christine M.
Sheibani, Nader
author_sort Zaitoun, Ismail S.
collection PubMed
description The visualization of choroidal vasculature and innate immune cells in the eyes of pigmented mice has been challenging due to the presence of a retinal pigment epithelium (RPE) layer separating the choroid and retina. Here, we established methods for visualizing the choroidal macrophages, mast cells, and vasculature in eyes of albino and pigmented mice using cell type-specific staining. We were able to visualize the choroidal arterial and venous systems. An arterial circle around the optic nerve was found in mice similar to the Zinn–Haller arterial circle that exists in humans and primates. Three different structural patterns of choriocapillaris were observed throughout the whole choroid: honeycomb-like, maze-like, and finger-like patterns. Choroidal mast cells were relatively few but dense around the optic nerve. Mast cell distribution in the middle and periphery was different among strains. Macrophages were found in all layers of the choroid. Thus, utilizing the simple and reliable methods described herein will allow the evaluation of transgenic and preclinical mouse models of ocular diseases that affect the choroid, including age-related macular degeneration (AMD), diabetic choroidopathy, and retinopathy of prematurity. These studies will advance our understanding of the pathophysiology, and molecular and cellular mechanisms that can be targeted therapeutically, in these diseases.
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spelling pubmed-96002922022-10-27 Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice Zaitoun, Ismail S. Song, Yong-Seok Zaitoun, Hammam B. Sorenson, Christine M. Sheibani, Nader Cells Article The visualization of choroidal vasculature and innate immune cells in the eyes of pigmented mice has been challenging due to the presence of a retinal pigment epithelium (RPE) layer separating the choroid and retina. Here, we established methods for visualizing the choroidal macrophages, mast cells, and vasculature in eyes of albino and pigmented mice using cell type-specific staining. We were able to visualize the choroidal arterial and venous systems. An arterial circle around the optic nerve was found in mice similar to the Zinn–Haller arterial circle that exists in humans and primates. Three different structural patterns of choriocapillaris were observed throughout the whole choroid: honeycomb-like, maze-like, and finger-like patterns. Choroidal mast cells were relatively few but dense around the optic nerve. Mast cell distribution in the middle and periphery was different among strains. Macrophages were found in all layers of the choroid. Thus, utilizing the simple and reliable methods described herein will allow the evaluation of transgenic and preclinical mouse models of ocular diseases that affect the choroid, including age-related macular degeneration (AMD), diabetic choroidopathy, and retinopathy of prematurity. These studies will advance our understanding of the pathophysiology, and molecular and cellular mechanisms that can be targeted therapeutically, in these diseases. MDPI 2022-10-21 /pmc/articles/PMC9600292/ /pubmed/36291198 http://dx.doi.org/10.3390/cells11203329 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zaitoun, Ismail S.
Song, Yong-Seok
Zaitoun, Hammam B.
Sorenson, Christine M.
Sheibani, Nader
Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice
title Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice
title_full Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice
title_fullStr Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice
title_full_unstemmed Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice
title_short Assessment of Choroidal Vasculature and Innate Immune Cells in the Eyes of Albino and Pigmented Mice
title_sort assessment of choroidal vasculature and innate immune cells in the eyes of albino and pigmented mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600292/
https://www.ncbi.nlm.nih.gov/pubmed/36291198
http://dx.doi.org/10.3390/cells11203329
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