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Plasmodium falciparum CRK5 Is Critical for Male Gametogenesis and Infection of the Mosquito

Cyclin-dependent kinases (CDKs) and cyclins are critical cell cycle regulators in eukaryotes. In this study, we functionally characterized a CDK-related kinase (CRK5) of the human malaria parasite Plasmodium falciparum. P. falciparum CRK5 (PfCRK5) was expressed in asexual blood stages and sexual gam...

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Detalles Bibliográficos
Autores principales: Kumar, Sudhir, Gargaro, Olivia R., Kappe, Stefan H. I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600428/
https://www.ncbi.nlm.nih.gov/pubmed/36154191
http://dx.doi.org/10.1128/mbio.02227-22
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author Kumar, Sudhir
Gargaro, Olivia R.
Kappe, Stefan H. I.
author_facet Kumar, Sudhir
Gargaro, Olivia R.
Kappe, Stefan H. I.
author_sort Kumar, Sudhir
collection PubMed
description Cyclin-dependent kinases (CDKs) and cyclins are critical cell cycle regulators in eukaryotes. In this study, we functionally characterized a CDK-related kinase (CRK5) of the human malaria parasite Plasmodium falciparum. P. falciparum CRK5 (PfCRK5) was expressed in asexual blood stages and sexual gametocyte stages, but showed male gametocyte- specific expression. In contrast to previous findings, we showed that gene deletion Pfcrk5(−) parasites grew normally as asexual stages and underwent normal gametocytogenesis to stage V gametocytes. However, Pfcrk5(−) parasites showed a severe defect in male gametogenesis, which was evident by a significant reduction in the emergence of male gametes (exflagellation). This defect caused a severe reduction of parasite transmission to the mosquito. Genetic crosses performed using sex-specific sterile transgenic parasites revealed that Pfcrk5(−) parasites suffered a defect in male fertility but female gametes were fertile. Taken together, these results demonstrate that PfCRK5 is a critical sexual stage kinase which regulates male gametogenesis and transmission to the mosquito.
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spelling pubmed-96004282022-10-27 Plasmodium falciparum CRK5 Is Critical for Male Gametogenesis and Infection of the Mosquito Kumar, Sudhir Gargaro, Olivia R. Kappe, Stefan H. I. mBio Research Article Cyclin-dependent kinases (CDKs) and cyclins are critical cell cycle regulators in eukaryotes. In this study, we functionally characterized a CDK-related kinase (CRK5) of the human malaria parasite Plasmodium falciparum. P. falciparum CRK5 (PfCRK5) was expressed in asexual blood stages and sexual gametocyte stages, but showed male gametocyte- specific expression. In contrast to previous findings, we showed that gene deletion Pfcrk5(−) parasites grew normally as asexual stages and underwent normal gametocytogenesis to stage V gametocytes. However, Pfcrk5(−) parasites showed a severe defect in male gametogenesis, which was evident by a significant reduction in the emergence of male gametes (exflagellation). This defect caused a severe reduction of parasite transmission to the mosquito. Genetic crosses performed using sex-specific sterile transgenic parasites revealed that Pfcrk5(−) parasites suffered a defect in male fertility but female gametes were fertile. Taken together, these results demonstrate that PfCRK5 is a critical sexual stage kinase which regulates male gametogenesis and transmission to the mosquito. American Society for Microbiology 2022-09-26 /pmc/articles/PMC9600428/ /pubmed/36154191 http://dx.doi.org/10.1128/mbio.02227-22 Text en Copyright © 2022 Kumar et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Kumar, Sudhir
Gargaro, Olivia R.
Kappe, Stefan H. I.
Plasmodium falciparum CRK5 Is Critical for Male Gametogenesis and Infection of the Mosquito
title Plasmodium falciparum CRK5 Is Critical for Male Gametogenesis and Infection of the Mosquito
title_full Plasmodium falciparum CRK5 Is Critical for Male Gametogenesis and Infection of the Mosquito
title_fullStr Plasmodium falciparum CRK5 Is Critical for Male Gametogenesis and Infection of the Mosquito
title_full_unstemmed Plasmodium falciparum CRK5 Is Critical for Male Gametogenesis and Infection of the Mosquito
title_short Plasmodium falciparum CRK5 Is Critical for Male Gametogenesis and Infection of the Mosquito
title_sort plasmodium falciparum crk5 is critical for male gametogenesis and infection of the mosquito
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600428/
https://www.ncbi.nlm.nih.gov/pubmed/36154191
http://dx.doi.org/10.1128/mbio.02227-22
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