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Suppressive Effects of 4-(Phenylsulfanyl) Butan-2-One on CCL-1 Production via Histone Acetylation in Monocytes

The 4-(phenylsulfanyl) butan-2-one (4-PSB-2), a marine-derived compound from soft coral, was proven to have multiple biological activities including neuroprotection and potent anti-inflammatory effects. CC chemokine ligand (CCL)-1 belongs to T helper (Th)2-related chemokines that are involved in the...

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Autores principales: Tsai, Ming-Kai, Tsai, Mei-Lan, Wen, Zhi-Hong, Liao, Wei-Ting, Lin, Yi-Ching, Chiou, Hsin-Ying Clair, Lin, Ming-Hong, Hung, Chih-Hsing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600508/
https://www.ncbi.nlm.nih.gov/pubmed/36286030
http://dx.doi.org/10.3390/cimb44100315
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author Tsai, Ming-Kai
Tsai, Mei-Lan
Wen, Zhi-Hong
Liao, Wei-Ting
Lin, Yi-Ching
Chiou, Hsin-Ying Clair
Lin, Ming-Hong
Hung, Chih-Hsing
author_facet Tsai, Ming-Kai
Tsai, Mei-Lan
Wen, Zhi-Hong
Liao, Wei-Ting
Lin, Yi-Ching
Chiou, Hsin-Ying Clair
Lin, Ming-Hong
Hung, Chih-Hsing
author_sort Tsai, Ming-Kai
collection PubMed
description The 4-(phenylsulfanyl) butan-2-one (4-PSB-2), a marine-derived compound from soft coral, was proven to have multiple biological activities including neuroprotection and potent anti-inflammatory effects. CC chemokine ligand (CCL)-1 belongs to T helper (Th)2-related chemokines that are involved in the recruitment of Th2 inflammatory cells. Histone acetylation has been recognized as a critical mechanism underlying the regulated cytokine and chemokine production. Our study tried to investigate the anti-inflammatory effect of 4-PSB-2 on CCL-1 production in human monocytes and explore possible underlying intracellular processes, including epigenetic regulation. To confirm our hypothesis, human monocyte THP-1 cell line and primary CD14(+) cells were pretreated with various concentrations of 4-PSB-2 and then were stimulated with lipopolysaccharide (LPS). The CCL-1 concentration was measured by enzyme-linked immunosorbent assays, and the intracellular signaling pathways and epigenetic regulation of 4-PSB-2 were investigated by using Western blotting and chromatin immunoprecipitation analysis. In this study, we found that 4-PSB-2 had a suppressive effect on LPS-induced CCL-1 production. Moreover, this suppressive effect of 4-PSB-2 was mediated via intracellular signaling such as the mitogen-activated protein kinase and nuclear factor-κB pathways. In addition, 4-PSB-2 could suppress CCL-1 production by epigenetic regulation through downregulating histone H3 and H4 acetylation. In short, our study demonstrated that 4-PSB-2 may have a potential role in the treatment of allergic inflammation.
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spelling pubmed-96005082022-10-27 Suppressive Effects of 4-(Phenylsulfanyl) Butan-2-One on CCL-1 Production via Histone Acetylation in Monocytes Tsai, Ming-Kai Tsai, Mei-Lan Wen, Zhi-Hong Liao, Wei-Ting Lin, Yi-Ching Chiou, Hsin-Ying Clair Lin, Ming-Hong Hung, Chih-Hsing Curr Issues Mol Biol Article The 4-(phenylsulfanyl) butan-2-one (4-PSB-2), a marine-derived compound from soft coral, was proven to have multiple biological activities including neuroprotection and potent anti-inflammatory effects. CC chemokine ligand (CCL)-1 belongs to T helper (Th)2-related chemokines that are involved in the recruitment of Th2 inflammatory cells. Histone acetylation has been recognized as a critical mechanism underlying the regulated cytokine and chemokine production. Our study tried to investigate the anti-inflammatory effect of 4-PSB-2 on CCL-1 production in human monocytes and explore possible underlying intracellular processes, including epigenetic regulation. To confirm our hypothesis, human monocyte THP-1 cell line and primary CD14(+) cells were pretreated with various concentrations of 4-PSB-2 and then were stimulated with lipopolysaccharide (LPS). The CCL-1 concentration was measured by enzyme-linked immunosorbent assays, and the intracellular signaling pathways and epigenetic regulation of 4-PSB-2 were investigated by using Western blotting and chromatin immunoprecipitation analysis. In this study, we found that 4-PSB-2 had a suppressive effect on LPS-induced CCL-1 production. Moreover, this suppressive effect of 4-PSB-2 was mediated via intracellular signaling such as the mitogen-activated protein kinase and nuclear factor-κB pathways. In addition, 4-PSB-2 could suppress CCL-1 production by epigenetic regulation through downregulating histone H3 and H4 acetylation. In short, our study demonstrated that 4-PSB-2 may have a potential role in the treatment of allergic inflammation. MDPI 2022-10-03 /pmc/articles/PMC9600508/ /pubmed/36286030 http://dx.doi.org/10.3390/cimb44100315 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tsai, Ming-Kai
Tsai, Mei-Lan
Wen, Zhi-Hong
Liao, Wei-Ting
Lin, Yi-Ching
Chiou, Hsin-Ying Clair
Lin, Ming-Hong
Hung, Chih-Hsing
Suppressive Effects of 4-(Phenylsulfanyl) Butan-2-One on CCL-1 Production via Histone Acetylation in Monocytes
title Suppressive Effects of 4-(Phenylsulfanyl) Butan-2-One on CCL-1 Production via Histone Acetylation in Monocytes
title_full Suppressive Effects of 4-(Phenylsulfanyl) Butan-2-One on CCL-1 Production via Histone Acetylation in Monocytes
title_fullStr Suppressive Effects of 4-(Phenylsulfanyl) Butan-2-One on CCL-1 Production via Histone Acetylation in Monocytes
title_full_unstemmed Suppressive Effects of 4-(Phenylsulfanyl) Butan-2-One on CCL-1 Production via Histone Acetylation in Monocytes
title_short Suppressive Effects of 4-(Phenylsulfanyl) Butan-2-One on CCL-1 Production via Histone Acetylation in Monocytes
title_sort suppressive effects of 4-(phenylsulfanyl) butan-2-one on ccl-1 production via histone acetylation in monocytes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600508/
https://www.ncbi.nlm.nih.gov/pubmed/36286030
http://dx.doi.org/10.3390/cimb44100315
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