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Bisphenol Exposure Disrupts Cytoskeletal Organization and Development of Pre-Implantation Embryos

The endocrine disrupting activity of bisphenol compounds is well documented, but less is known regarding their impact on cell division and early embryo formation. Here, we tested the effects of acute in vitro exposure to bisphenol A (BPA) and its common substitute, bisphenol F (BPF), during critical...

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Autores principales: Yang, Luhan, Baumann, Claudia, De La Fuente, Rabindranath, Viveiros, Maria M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600733/
https://www.ncbi.nlm.nih.gov/pubmed/36291100
http://dx.doi.org/10.3390/cells11203233
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author Yang, Luhan
Baumann, Claudia
De La Fuente, Rabindranath
Viveiros, Maria M.
author_facet Yang, Luhan
Baumann, Claudia
De La Fuente, Rabindranath
Viveiros, Maria M.
author_sort Yang, Luhan
collection PubMed
description The endocrine disrupting activity of bisphenol compounds is well documented, but less is known regarding their impact on cell division and early embryo formation. Here, we tested the effects of acute in vitro exposure to bisphenol A (BPA) and its common substitute, bisphenol F (BPF), during critical stages of mouse pre-implantation embryo development, including the first mitotic division, cell polarization, as well as morula and blastocyst formation. Timing of initial cleavage was determined by live-cell imaging, while subsequent divisions, cytoskeletal organization and lineage marker labeling were assessed by high-resolution fluorescence microscopy. Our analysis reveals that brief culture with BPA or BPF impeded cell division and disrupted embryo development at all stages tested. Surprisingly, BPF was more detrimental to the early embryo than BPA. Notably, poor embryo development was associated with cytoskeletal disruptions of the actomyosin network, apical domain formation during cell polarization, actin ring zippering for embryo sealing and altered cell lineage marker profiles. These results underscore that bisphenols can disrupt cytoskeletal integrity and remodeling that is vital for early embryo development and raise concerns regarding the use of BPF as a ‘safe’ BPA substitute.
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spelling pubmed-96007332022-10-27 Bisphenol Exposure Disrupts Cytoskeletal Organization and Development of Pre-Implantation Embryos Yang, Luhan Baumann, Claudia De La Fuente, Rabindranath Viveiros, Maria M. Cells Article The endocrine disrupting activity of bisphenol compounds is well documented, but less is known regarding their impact on cell division and early embryo formation. Here, we tested the effects of acute in vitro exposure to bisphenol A (BPA) and its common substitute, bisphenol F (BPF), during critical stages of mouse pre-implantation embryo development, including the first mitotic division, cell polarization, as well as morula and blastocyst formation. Timing of initial cleavage was determined by live-cell imaging, while subsequent divisions, cytoskeletal organization and lineage marker labeling were assessed by high-resolution fluorescence microscopy. Our analysis reveals that brief culture with BPA or BPF impeded cell division and disrupted embryo development at all stages tested. Surprisingly, BPF was more detrimental to the early embryo than BPA. Notably, poor embryo development was associated with cytoskeletal disruptions of the actomyosin network, apical domain formation during cell polarization, actin ring zippering for embryo sealing and altered cell lineage marker profiles. These results underscore that bisphenols can disrupt cytoskeletal integrity and remodeling that is vital for early embryo development and raise concerns regarding the use of BPF as a ‘safe’ BPA substitute. MDPI 2022-10-14 /pmc/articles/PMC9600733/ /pubmed/36291100 http://dx.doi.org/10.3390/cells11203233 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yang, Luhan
Baumann, Claudia
De La Fuente, Rabindranath
Viveiros, Maria M.
Bisphenol Exposure Disrupts Cytoskeletal Organization and Development of Pre-Implantation Embryos
title Bisphenol Exposure Disrupts Cytoskeletal Organization and Development of Pre-Implantation Embryos
title_full Bisphenol Exposure Disrupts Cytoskeletal Organization and Development of Pre-Implantation Embryos
title_fullStr Bisphenol Exposure Disrupts Cytoskeletal Organization and Development of Pre-Implantation Embryos
title_full_unstemmed Bisphenol Exposure Disrupts Cytoskeletal Organization and Development of Pre-Implantation Embryos
title_short Bisphenol Exposure Disrupts Cytoskeletal Organization and Development of Pre-Implantation Embryos
title_sort bisphenol exposure disrupts cytoskeletal organization and development of pre-implantation embryos
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600733/
https://www.ncbi.nlm.nih.gov/pubmed/36291100
http://dx.doi.org/10.3390/cells11203233
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