Cargando…

Determination of the Accuracy of Salivary Biomarkers for Periodontal Diagnosis

Background: We aimed to investigate the accuracy of salivary matrix metalloproteinases (MMP)-8 and -9, and tissue inhibitor of metalloproteinase (TIMP)-1 in diagnosing periodontitis and in distinguishing periodontitis stages (S)1 to S3. Methods: This study was a case–control study that included pati...

Descripción completa

Detalles Bibliográficos
Autores principales: Mohammed, Hiba Abdullah, Abdulkareem, Ali Abbas, Zardawi, Faraedon Mostafa, Gul, Sarhang Sarwat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600931/
https://www.ncbi.nlm.nih.gov/pubmed/36292174
http://dx.doi.org/10.3390/diagnostics12102485
_version_ 1784816952804376576
author Mohammed, Hiba Abdullah
Abdulkareem, Ali Abbas
Zardawi, Faraedon Mostafa
Gul, Sarhang Sarwat
author_facet Mohammed, Hiba Abdullah
Abdulkareem, Ali Abbas
Zardawi, Faraedon Mostafa
Gul, Sarhang Sarwat
author_sort Mohammed, Hiba Abdullah
collection PubMed
description Background: We aimed to investigate the accuracy of salivary matrix metalloproteinases (MMP)-8 and -9, and tissue inhibitor of metalloproteinase (TIMP)-1 in diagnosing periodontitis and in distinguishing periodontitis stages (S)1 to S3. Methods: This study was a case–control study that included patients with periodontitis S1 to S3 and subjects with healthy periodontia (controls). Saliva was collected, and then, clinical parameters were recorded, including plaque index, bleeding on probing, probing pocket depth, and clinical attachment level. Diagnosis was confirmed by assessing the alveolar bone level using radiography. Salivary biomarkers were assayed using an enzyme-linked immunosorbent assay. Results: A total of 45 patients (15 for each stage) and 18 healthy subjects as controls were included. The levels of all salivary biomarkers and clinical parameters were significantly higher in periodontitis subjects than in the controls. The ROC curve showed that MMP-8, MMP-9, TIMP-1, MMP-8/TIMP-1, and MMP-9/TIMP-1 had statistically significant diagnostic accuracy, with areas under the curve (AUCs) of 0.892, 0.844, 0.920, 0.986, and 1.000, respectively, when distinguishing periodontitis from the controls. Similarly, these biomarkers showed significant diagnostic accuracy in the differentiation of S1 periodontitis from the controls (AUC range from 0.902 to 1.000). Conclusions: This study suggested that salivary biomarkers exhibited high diagnostic accuracy in distinguishing periodontal health from periodontitis in general as well as S1 periodontitis. Furthermore, TIMP-1 could differentiate S1 from S3.
format Online
Article
Text
id pubmed-9600931
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96009312022-10-27 Determination of the Accuracy of Salivary Biomarkers for Periodontal Diagnosis Mohammed, Hiba Abdullah Abdulkareem, Ali Abbas Zardawi, Faraedon Mostafa Gul, Sarhang Sarwat Diagnostics (Basel) Article Background: We aimed to investigate the accuracy of salivary matrix metalloproteinases (MMP)-8 and -9, and tissue inhibitor of metalloproteinase (TIMP)-1 in diagnosing periodontitis and in distinguishing periodontitis stages (S)1 to S3. Methods: This study was a case–control study that included patients with periodontitis S1 to S3 and subjects with healthy periodontia (controls). Saliva was collected, and then, clinical parameters were recorded, including plaque index, bleeding on probing, probing pocket depth, and clinical attachment level. Diagnosis was confirmed by assessing the alveolar bone level using radiography. Salivary biomarkers were assayed using an enzyme-linked immunosorbent assay. Results: A total of 45 patients (15 for each stage) and 18 healthy subjects as controls were included. The levels of all salivary biomarkers and clinical parameters were significantly higher in periodontitis subjects than in the controls. The ROC curve showed that MMP-8, MMP-9, TIMP-1, MMP-8/TIMP-1, and MMP-9/TIMP-1 had statistically significant diagnostic accuracy, with areas under the curve (AUCs) of 0.892, 0.844, 0.920, 0.986, and 1.000, respectively, when distinguishing periodontitis from the controls. Similarly, these biomarkers showed significant diagnostic accuracy in the differentiation of S1 periodontitis from the controls (AUC range from 0.902 to 1.000). Conclusions: This study suggested that salivary biomarkers exhibited high diagnostic accuracy in distinguishing periodontal health from periodontitis in general as well as S1 periodontitis. Furthermore, TIMP-1 could differentiate S1 from S3. MDPI 2022-10-14 /pmc/articles/PMC9600931/ /pubmed/36292174 http://dx.doi.org/10.3390/diagnostics12102485 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mohammed, Hiba Abdullah
Abdulkareem, Ali Abbas
Zardawi, Faraedon Mostafa
Gul, Sarhang Sarwat
Determination of the Accuracy of Salivary Biomarkers for Periodontal Diagnosis
title Determination of the Accuracy of Salivary Biomarkers for Periodontal Diagnosis
title_full Determination of the Accuracy of Salivary Biomarkers for Periodontal Diagnosis
title_fullStr Determination of the Accuracy of Salivary Biomarkers for Periodontal Diagnosis
title_full_unstemmed Determination of the Accuracy of Salivary Biomarkers for Periodontal Diagnosis
title_short Determination of the Accuracy of Salivary Biomarkers for Periodontal Diagnosis
title_sort determination of the accuracy of salivary biomarkers for periodontal diagnosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9600931/
https://www.ncbi.nlm.nih.gov/pubmed/36292174
http://dx.doi.org/10.3390/diagnostics12102485
work_keys_str_mv AT mohammedhibaabdullah determinationoftheaccuracyofsalivarybiomarkersforperiodontaldiagnosis
AT abdulkareemaliabbas determinationoftheaccuracyofsalivarybiomarkersforperiodontaldiagnosis
AT zardawifaraedonmostafa determinationoftheaccuracyofsalivarybiomarkersforperiodontaldiagnosis
AT gulsarhangsarwat determinationoftheaccuracyofsalivarybiomarkersforperiodontaldiagnosis