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Saturation mutagenesis of a predicted ancestral Syk‐family kinase

Many tyrosine kinases cannot be expressed readily in Escherichia coli, limiting facile production of these proteins for biochemical experiments. We used ancestral sequence reconstruction to generate a spleen tyrosine kinase (Syk) variant that can be expressed in bacteria and purified in soluble form...

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Detalles Bibliográficos
Autores principales:	Hobbs, Helen T., Shah, Neel H., Shoemaker, Sophie R., Amacher, Jeanine F., Marqusee, Susan, Kuriyan, John
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley & Sons, Inc. 2022
Materias:	Full‐length Papers
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9601881/ https://www.ncbi.nlm.nih.gov/pubmed/36173161 http://dx.doi.org/10.1002/pro.4411

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