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Robocasting and Laser Micromachining of Sol-Gel Derived 3D Silica/Gelatin/β-TCP Scaffolds for Bone Tissue Regeneration

The design and synthesis of sol-gel silica-based hybrid materials and composites offer significant benefits to obtain innovative biomaterials with controlled porosity at the nanostructure level for applications in bone tissue engineering. In this work, the combination of robocasting with sol-gel ink...

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Autores principales: Reyes-Peces, María V., Félix, Eduardo, Martínez-Vázquez, Francisco J., Fernández-Montesinos, Rafael, Bomati-Miguel, Óscar, Mesa-Díaz, María del Mar, Alcántara, Rodrigo, Vilches-Pérez, José Ignacio, Salido, Mercedes, De la Rosa-Fox, Nicolás, Piñero, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9602064/
https://www.ncbi.nlm.nih.gov/pubmed/36286135
http://dx.doi.org/10.3390/gels8100634
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author Reyes-Peces, María V.
Félix, Eduardo
Martínez-Vázquez, Francisco J.
Fernández-Montesinos, Rafael
Bomati-Miguel, Óscar
Mesa-Díaz, María del Mar
Alcántara, Rodrigo
Vilches-Pérez, José Ignacio
Salido, Mercedes
De la Rosa-Fox, Nicolás
Piñero, Manuel
author_facet Reyes-Peces, María V.
Félix, Eduardo
Martínez-Vázquez, Francisco J.
Fernández-Montesinos, Rafael
Bomati-Miguel, Óscar
Mesa-Díaz, María del Mar
Alcántara, Rodrigo
Vilches-Pérez, José Ignacio
Salido, Mercedes
De la Rosa-Fox, Nicolás
Piñero, Manuel
author_sort Reyes-Peces, María V.
collection PubMed
description The design and synthesis of sol-gel silica-based hybrid materials and composites offer significant benefits to obtain innovative biomaterials with controlled porosity at the nanostructure level for applications in bone tissue engineering. In this work, the combination of robocasting with sol-gel ink of suitable viscosity prepared by mixing tetraethoxysilane (TEOS), gelatin and β-tricalcium phosphate (β-TCP) allowed for the manufacture of 3D scaffolds consisting of a 3D square mesh of interpenetrating rods, with macropore size of 354.0 ± 17.0 μm, without the use of chemical additives at room temperature. The silica/gelatin/β-TCP system underwent irreversible gelation, and the resulting gels were also used to fabricate different 3D structures by means of an alternative scaffolding method, involving high-resolution laser micromachining by laser ablation. By this way, 3D scaffolds made of 2 mm thick rectangular prisms presenting a parallel macropore system drilled through the whole thickness and consisting of laser micromachined holes of 350.8 ± 16.6-micrometer diameter, whose centers were spaced 1312.0 ± 23.0 μm, were created. Both sol-gel based 3D scaffold configurations combined compressive strength in the range of 2–3 MPa and the biocompatibility of the hybrid material. In addition, the observed Si, Ca and P biodegradation provided a suitable microenvironment with significant focal adhesion development, maturation and also enhanced in vitro cell growth. In conclusion, this work successfully confirmed the feasibility of both strategies for the fabrication of new sol-gel-based hybrid scaffolds with osteoconductive properties.
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spelling pubmed-96020642022-10-27 Robocasting and Laser Micromachining of Sol-Gel Derived 3D Silica/Gelatin/β-TCP Scaffolds for Bone Tissue Regeneration Reyes-Peces, María V. Félix, Eduardo Martínez-Vázquez, Francisco J. Fernández-Montesinos, Rafael Bomati-Miguel, Óscar Mesa-Díaz, María del Mar Alcántara, Rodrigo Vilches-Pérez, José Ignacio Salido, Mercedes De la Rosa-Fox, Nicolás Piñero, Manuel Gels Article The design and synthesis of sol-gel silica-based hybrid materials and composites offer significant benefits to obtain innovative biomaterials with controlled porosity at the nanostructure level for applications in bone tissue engineering. In this work, the combination of robocasting with sol-gel ink of suitable viscosity prepared by mixing tetraethoxysilane (TEOS), gelatin and β-tricalcium phosphate (β-TCP) allowed for the manufacture of 3D scaffolds consisting of a 3D square mesh of interpenetrating rods, with macropore size of 354.0 ± 17.0 μm, without the use of chemical additives at room temperature. The silica/gelatin/β-TCP system underwent irreversible gelation, and the resulting gels were also used to fabricate different 3D structures by means of an alternative scaffolding method, involving high-resolution laser micromachining by laser ablation. By this way, 3D scaffolds made of 2 mm thick rectangular prisms presenting a parallel macropore system drilled through the whole thickness and consisting of laser micromachined holes of 350.8 ± 16.6-micrometer diameter, whose centers were spaced 1312.0 ± 23.0 μm, were created. Both sol-gel based 3D scaffold configurations combined compressive strength in the range of 2–3 MPa and the biocompatibility of the hybrid material. In addition, the observed Si, Ca and P biodegradation provided a suitable microenvironment with significant focal adhesion development, maturation and also enhanced in vitro cell growth. In conclusion, this work successfully confirmed the feasibility of both strategies for the fabrication of new sol-gel-based hybrid scaffolds with osteoconductive properties. MDPI 2022-10-07 /pmc/articles/PMC9602064/ /pubmed/36286135 http://dx.doi.org/10.3390/gels8100634 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Reyes-Peces, María V.
Félix, Eduardo
Martínez-Vázquez, Francisco J.
Fernández-Montesinos, Rafael
Bomati-Miguel, Óscar
Mesa-Díaz, María del Mar
Alcántara, Rodrigo
Vilches-Pérez, José Ignacio
Salido, Mercedes
De la Rosa-Fox, Nicolás
Piñero, Manuel
Robocasting and Laser Micromachining of Sol-Gel Derived 3D Silica/Gelatin/β-TCP Scaffolds for Bone Tissue Regeneration
title Robocasting and Laser Micromachining of Sol-Gel Derived 3D Silica/Gelatin/β-TCP Scaffolds for Bone Tissue Regeneration
title_full Robocasting and Laser Micromachining of Sol-Gel Derived 3D Silica/Gelatin/β-TCP Scaffolds for Bone Tissue Regeneration
title_fullStr Robocasting and Laser Micromachining of Sol-Gel Derived 3D Silica/Gelatin/β-TCP Scaffolds for Bone Tissue Regeneration
title_full_unstemmed Robocasting and Laser Micromachining of Sol-Gel Derived 3D Silica/Gelatin/β-TCP Scaffolds for Bone Tissue Regeneration
title_short Robocasting and Laser Micromachining of Sol-Gel Derived 3D Silica/Gelatin/β-TCP Scaffolds for Bone Tissue Regeneration
title_sort robocasting and laser micromachining of sol-gel derived 3d silica/gelatin/β-tcp scaffolds for bone tissue regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9602064/
https://www.ncbi.nlm.nih.gov/pubmed/36286135
http://dx.doi.org/10.3390/gels8100634
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