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Culturomics to Investigate the Endometrial Microbiome: Proof-of-Concept

The microbiome of the reproductive tract has been associated with (sub)fertility and it has been suggested that dysbiosis reduces success rates and pregnancy outcomes. The endometrial microbiome is of particular interest given the potential impact on the embryo implantation. To date, all endometrial...

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Autores principales: Vanstokstraeten, Robin, Mackens, Shari, Callewaert, Ellen, Blotwijk, Susanne, Emmerechts, Kristof, Crombé, Florence, Soetens, Oriane, Wybo, Ingrid, Vandoorslaer, Kristof, Mostert, Laurence, De Geyter, Deborah, Muyldermans, Astrid, Blockeel, Christophe, Piérard, Denis, Demuyser, Thomas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9602868/
https://www.ncbi.nlm.nih.gov/pubmed/36293066
http://dx.doi.org/10.3390/ijms232012212
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author Vanstokstraeten, Robin
Mackens, Shari
Callewaert, Ellen
Blotwijk, Susanne
Emmerechts, Kristof
Crombé, Florence
Soetens, Oriane
Wybo, Ingrid
Vandoorslaer, Kristof
Mostert, Laurence
De Geyter, Deborah
Muyldermans, Astrid
Blockeel, Christophe
Piérard, Denis
Demuyser, Thomas
author_facet Vanstokstraeten, Robin
Mackens, Shari
Callewaert, Ellen
Blotwijk, Susanne
Emmerechts, Kristof
Crombé, Florence
Soetens, Oriane
Wybo, Ingrid
Vandoorslaer, Kristof
Mostert, Laurence
De Geyter, Deborah
Muyldermans, Astrid
Blockeel, Christophe
Piérard, Denis
Demuyser, Thomas
author_sort Vanstokstraeten, Robin
collection PubMed
description The microbiome of the reproductive tract has been associated with (sub)fertility and it has been suggested that dysbiosis reduces success rates and pregnancy outcomes. The endometrial microbiome is of particular interest given the potential impact on the embryo implantation. To date, all endometrial microbiome studies have applied a metagenomics approach. A sequencing-based technique, however, has its limitations, more specifically in adequately exploring low-biomass settings, such as intra-uterine/endometrial samples. In this proof-of-concept study, we demonstrate the applicability of culturomics, a high-throughput culturing approach, to investigate the endometrial microbiome. Ten subfertile women undergoing diagnostic hysteroscopy and endometrial biopsy, as part of their routine work-up at Brussels IVF, were included after their informed consent. Biopsies were used to culture microbiota for up to 30 days in multiple aerobic and anaerobic conditions. Subsequent WASPLab(®)-assisted culturomics enabled a standardized methodology. Matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS) or 16S rRNA sequencing was applied to identify all of bacterial and fungal isolates. Eighty-three bacterial and two fungal species were identified. The detected species were in concordance with previously published metagenomics-based endometrial microbiota analyses as 77 (91%) of them belonged to previously described genera. Nevertheless, highlighting the added value of culturomics to identify most isolates at the species level, 53 (62.4%) of the identified species were described in the endometrial microbiota for the first time. This study shows the applicability and added value of WASPLab(®)-assisted culturomics to investigate the low biomass endometrial microbiome at a species level.
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spelling pubmed-96028682022-10-27 Culturomics to Investigate the Endometrial Microbiome: Proof-of-Concept Vanstokstraeten, Robin Mackens, Shari Callewaert, Ellen Blotwijk, Susanne Emmerechts, Kristof Crombé, Florence Soetens, Oriane Wybo, Ingrid Vandoorslaer, Kristof Mostert, Laurence De Geyter, Deborah Muyldermans, Astrid Blockeel, Christophe Piérard, Denis Demuyser, Thomas Int J Mol Sci Article The microbiome of the reproductive tract has been associated with (sub)fertility and it has been suggested that dysbiosis reduces success rates and pregnancy outcomes. The endometrial microbiome is of particular interest given the potential impact on the embryo implantation. To date, all endometrial microbiome studies have applied a metagenomics approach. A sequencing-based technique, however, has its limitations, more specifically in adequately exploring low-biomass settings, such as intra-uterine/endometrial samples. In this proof-of-concept study, we demonstrate the applicability of culturomics, a high-throughput culturing approach, to investigate the endometrial microbiome. Ten subfertile women undergoing diagnostic hysteroscopy and endometrial biopsy, as part of their routine work-up at Brussels IVF, were included after their informed consent. Biopsies were used to culture microbiota for up to 30 days in multiple aerobic and anaerobic conditions. Subsequent WASPLab(®)-assisted culturomics enabled a standardized methodology. Matrix-assisted laser desorption/ionization–time of flight mass spectrometry (MALDI-TOF MS) or 16S rRNA sequencing was applied to identify all of bacterial and fungal isolates. Eighty-three bacterial and two fungal species were identified. The detected species were in concordance with previously published metagenomics-based endometrial microbiota analyses as 77 (91%) of them belonged to previously described genera. Nevertheless, highlighting the added value of culturomics to identify most isolates at the species level, 53 (62.4%) of the identified species were described in the endometrial microbiota for the first time. This study shows the applicability and added value of WASPLab(®)-assisted culturomics to investigate the low biomass endometrial microbiome at a species level. MDPI 2022-10-13 /pmc/articles/PMC9602868/ /pubmed/36293066 http://dx.doi.org/10.3390/ijms232012212 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Vanstokstraeten, Robin
Mackens, Shari
Callewaert, Ellen
Blotwijk, Susanne
Emmerechts, Kristof
Crombé, Florence
Soetens, Oriane
Wybo, Ingrid
Vandoorslaer, Kristof
Mostert, Laurence
De Geyter, Deborah
Muyldermans, Astrid
Blockeel, Christophe
Piérard, Denis
Demuyser, Thomas
Culturomics to Investigate the Endometrial Microbiome: Proof-of-Concept
title Culturomics to Investigate the Endometrial Microbiome: Proof-of-Concept
title_full Culturomics to Investigate the Endometrial Microbiome: Proof-of-Concept
title_fullStr Culturomics to Investigate the Endometrial Microbiome: Proof-of-Concept
title_full_unstemmed Culturomics to Investigate the Endometrial Microbiome: Proof-of-Concept
title_short Culturomics to Investigate the Endometrial Microbiome: Proof-of-Concept
title_sort culturomics to investigate the endometrial microbiome: proof-of-concept
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9602868/
https://www.ncbi.nlm.nih.gov/pubmed/36293066
http://dx.doi.org/10.3390/ijms232012212
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