Cargando…
Soil Bacterial Community May Offer Solutions for Ginger Cultivation
The Taitung region is one of Taiwan’s main sites for ginger agriculture. Due to issues with disease and nutrients, farmers cannot use continuous cropping techniques on ginger, meaning that the ginger industry is constantly searching for new land. Continuous cropping increases the risk of infection b...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9603371/ https://www.ncbi.nlm.nih.gov/pubmed/36098526 http://dx.doi.org/10.1128/spectrum.01803-22 |
_version_ | 1784817531329970176 |
---|---|
author | Wang, Chih-Wei Michelle Wong, Jing-Wen Yeh, Shu-Shuo Eric Hsieh, Yunli Tseng, Ching-Hung Yang, Shan-Hua Tang, Sen-Lin |
author_facet | Wang, Chih-Wei Michelle Wong, Jing-Wen Yeh, Shu-Shuo Eric Hsieh, Yunli Tseng, Ching-Hung Yang, Shan-Hua Tang, Sen-Lin |
author_sort | Wang, Chih-Wei |
collection | PubMed |
description | The Taitung region is one of Taiwan’s main sites for ginger agriculture. Due to issues with disease and nutrients, farmers cannot use continuous cropping techniques on ginger, meaning that the ginger industry is constantly searching for new land. Continuous cropping increases the risk of infection by Pythium myriotylum and Ralstonia solanacearum, which cause soft rot disease and bacterial wilt, respectively. In addition, fertilizer additives, which are commonly used to increase trace elements in the soil, cannot restore the soil when it is undergoing continuous cropping on ginger, even when there has been no observable decrease in trace elements in the soil. Recent studies about soil microbiome manipulation and the application of microorganisms have shown that plant-associated microbes have the ability to improve plant growth and facilitate sustainable agriculture, but studies of this kind still need to be carried out on ginger cultivation. Therefore, in this study, we used the bacterial 16S V3–V4 hypervariable region of the 16S rRNA region to investigate microbe compositions in ginger soil to identify the difference between ginger soil with and without disease. Later, to investigate the influence of the well-known biocontrol agent B. velezensis and the fungicide Etridiazole on soil microbes and ginger productivity, we designed an experiment that collected the soil samples according to the different periods of ginger cultivation to examine the microbial community dynamics in the rhizome and bulk soil. We demonstrated that B. velezensis is beneficial to ginger reproduction. In accordance with our results, we suggest that B. velezensis may influence the plant’s growth by adjusting its soil microbial composition. Etridiazole, on the other hand, may have some side effects on the ginger or beneficial bacteria in the soils that inhibit ginger reproduction. IMPORTANCE Pythium myriotylum and Ralstonia solanacearum cause soft rot disease and bacterial wilt, respectively. In this study, we used the bacterial 16S V3–V4 hypervariable region of the 16S rRNA region to investigate microbe compositions in healthy and diseased ginger soil and find out the influence of the well-known biocontrol agent B. velezensis and the fungicide Etridiazole on soil microbes and ginger productivity. These results demonstrated that B. velezensis benefits ginger reproduction and may influence the soil bacterial composition, while Etridiazole may have some side effects on the ginger or beneficial bacteria in the soils. The interactions among ginger, biocontrol agents, and fungicides need to be further investigated. |
format | Online Article Text |
id | pubmed-9603371 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-96033712022-10-27 Soil Bacterial Community May Offer Solutions for Ginger Cultivation Wang, Chih-Wei Michelle Wong, Jing-Wen Yeh, Shu-Shuo Eric Hsieh, Yunli Tseng, Ching-Hung Yang, Shan-Hua Tang, Sen-Lin Microbiol Spectr Research Article The Taitung region is one of Taiwan’s main sites for ginger agriculture. Due to issues with disease and nutrients, farmers cannot use continuous cropping techniques on ginger, meaning that the ginger industry is constantly searching for new land. Continuous cropping increases the risk of infection by Pythium myriotylum and Ralstonia solanacearum, which cause soft rot disease and bacterial wilt, respectively. In addition, fertilizer additives, which are commonly used to increase trace elements in the soil, cannot restore the soil when it is undergoing continuous cropping on ginger, even when there has been no observable decrease in trace elements in the soil. Recent studies about soil microbiome manipulation and the application of microorganisms have shown that plant-associated microbes have the ability to improve plant growth and facilitate sustainable agriculture, but studies of this kind still need to be carried out on ginger cultivation. Therefore, in this study, we used the bacterial 16S V3–V4 hypervariable region of the 16S rRNA region to investigate microbe compositions in ginger soil to identify the difference between ginger soil with and without disease. Later, to investigate the influence of the well-known biocontrol agent B. velezensis and the fungicide Etridiazole on soil microbes and ginger productivity, we designed an experiment that collected the soil samples according to the different periods of ginger cultivation to examine the microbial community dynamics in the rhizome and bulk soil. We demonstrated that B. velezensis is beneficial to ginger reproduction. In accordance with our results, we suggest that B. velezensis may influence the plant’s growth by adjusting its soil microbial composition. Etridiazole, on the other hand, may have some side effects on the ginger or beneficial bacteria in the soils that inhibit ginger reproduction. IMPORTANCE Pythium myriotylum and Ralstonia solanacearum cause soft rot disease and bacterial wilt, respectively. In this study, we used the bacterial 16S V3–V4 hypervariable region of the 16S rRNA region to investigate microbe compositions in healthy and diseased ginger soil and find out the influence of the well-known biocontrol agent B. velezensis and the fungicide Etridiazole on soil microbes and ginger productivity. These results demonstrated that B. velezensis benefits ginger reproduction and may influence the soil bacterial composition, while Etridiazole may have some side effects on the ginger or beneficial bacteria in the soils. The interactions among ginger, biocontrol agents, and fungicides need to be further investigated. American Society for Microbiology 2022-09-13 /pmc/articles/PMC9603371/ /pubmed/36098526 http://dx.doi.org/10.1128/spectrum.01803-22 Text en Copyright © 2022 Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Wang, Chih-Wei Michelle Wong, Jing-Wen Yeh, Shu-Shuo Eric Hsieh, Yunli Tseng, Ching-Hung Yang, Shan-Hua Tang, Sen-Lin Soil Bacterial Community May Offer Solutions for Ginger Cultivation |
title | Soil Bacterial Community May Offer Solutions for Ginger Cultivation |
title_full | Soil Bacterial Community May Offer Solutions for Ginger Cultivation |
title_fullStr | Soil Bacterial Community May Offer Solutions for Ginger Cultivation |
title_full_unstemmed | Soil Bacterial Community May Offer Solutions for Ginger Cultivation |
title_short | Soil Bacterial Community May Offer Solutions for Ginger Cultivation |
title_sort | soil bacterial community may offer solutions for ginger cultivation |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9603371/ https://www.ncbi.nlm.nih.gov/pubmed/36098526 http://dx.doi.org/10.1128/spectrum.01803-22 |
work_keys_str_mv | AT wangchihwei soilbacterialcommunitymayoffersolutionsforgingercultivation AT michellewongjingwen soilbacterialcommunitymayoffersolutionsforgingercultivation AT yehshushuo soilbacterialcommunitymayoffersolutionsforgingercultivation AT erichsiehyunli soilbacterialcommunitymayoffersolutionsforgingercultivation AT tsengchinghung soilbacterialcommunitymayoffersolutionsforgingercultivation AT yangshanhua soilbacterialcommunitymayoffersolutionsforgingercultivation AT tangsenlin soilbacterialcommunitymayoffersolutionsforgingercultivation |