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Soil Bacterial Community May Offer Solutions for Ginger Cultivation

The Taitung region is one of Taiwan’s main sites for ginger agriculture. Due to issues with disease and nutrients, farmers cannot use continuous cropping techniques on ginger, meaning that the ginger industry is constantly searching for new land. Continuous cropping increases the risk of infection b...

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Autores principales: Wang, Chih-Wei, Michelle Wong, Jing-Wen, Yeh, Shu-Shuo, Eric Hsieh, Yunli, Tseng, Ching-Hung, Yang, Shan-Hua, Tang, Sen-Lin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9603371/
https://www.ncbi.nlm.nih.gov/pubmed/36098526
http://dx.doi.org/10.1128/spectrum.01803-22
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author Wang, Chih-Wei
Michelle Wong, Jing-Wen
Yeh, Shu-Shuo
Eric Hsieh, Yunli
Tseng, Ching-Hung
Yang, Shan-Hua
Tang, Sen-Lin
author_facet Wang, Chih-Wei
Michelle Wong, Jing-Wen
Yeh, Shu-Shuo
Eric Hsieh, Yunli
Tseng, Ching-Hung
Yang, Shan-Hua
Tang, Sen-Lin
author_sort Wang, Chih-Wei
collection PubMed
description The Taitung region is one of Taiwan’s main sites for ginger agriculture. Due to issues with disease and nutrients, farmers cannot use continuous cropping techniques on ginger, meaning that the ginger industry is constantly searching for new land. Continuous cropping increases the risk of infection by Pythium myriotylum and Ralstonia solanacearum, which cause soft rot disease and bacterial wilt, respectively. In addition, fertilizer additives, which are commonly used to increase trace elements in the soil, cannot restore the soil when it is undergoing continuous cropping on ginger, even when there has been no observable decrease in trace elements in the soil. Recent studies about soil microbiome manipulation and the application of microorganisms have shown that plant-associated microbes have the ability to improve plant growth and facilitate sustainable agriculture, but studies of this kind still need to be carried out on ginger cultivation. Therefore, in this study, we used the bacterial 16S V3–V4 hypervariable region of the 16S rRNA region to investigate microbe compositions in ginger soil to identify the difference between ginger soil with and without disease. Later, to investigate the influence of the well-known biocontrol agent B. velezensis and the fungicide Etridiazole on soil microbes and ginger productivity, we designed an experiment that collected the soil samples according to the different periods of ginger cultivation to examine the microbial community dynamics in the rhizome and bulk soil. We demonstrated that B. velezensis is beneficial to ginger reproduction. In accordance with our results, we suggest that B. velezensis may influence the plant’s growth by adjusting its soil microbial composition. Etridiazole, on the other hand, may have some side effects on the ginger or beneficial bacteria in the soils that inhibit ginger reproduction. IMPORTANCE Pythium myriotylum and Ralstonia solanacearum cause soft rot disease and bacterial wilt, respectively. In this study, we used the bacterial 16S V3–V4 hypervariable region of the 16S rRNA region to investigate microbe compositions in healthy and diseased ginger soil and find out the influence of the well-known biocontrol agent B. velezensis and the fungicide Etridiazole on soil microbes and ginger productivity. These results demonstrated that B. velezensis benefits ginger reproduction and may influence the soil bacterial composition, while Etridiazole may have some side effects on the ginger or beneficial bacteria in the soils. The interactions among ginger, biocontrol agents, and fungicides need to be further investigated.
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spelling pubmed-96033712022-10-27 Soil Bacterial Community May Offer Solutions for Ginger Cultivation Wang, Chih-Wei Michelle Wong, Jing-Wen Yeh, Shu-Shuo Eric Hsieh, Yunli Tseng, Ching-Hung Yang, Shan-Hua Tang, Sen-Lin Microbiol Spectr Research Article The Taitung region is one of Taiwan’s main sites for ginger agriculture. Due to issues with disease and nutrients, farmers cannot use continuous cropping techniques on ginger, meaning that the ginger industry is constantly searching for new land. Continuous cropping increases the risk of infection by Pythium myriotylum and Ralstonia solanacearum, which cause soft rot disease and bacterial wilt, respectively. In addition, fertilizer additives, which are commonly used to increase trace elements in the soil, cannot restore the soil when it is undergoing continuous cropping on ginger, even when there has been no observable decrease in trace elements in the soil. Recent studies about soil microbiome manipulation and the application of microorganisms have shown that plant-associated microbes have the ability to improve plant growth and facilitate sustainable agriculture, but studies of this kind still need to be carried out on ginger cultivation. Therefore, in this study, we used the bacterial 16S V3–V4 hypervariable region of the 16S rRNA region to investigate microbe compositions in ginger soil to identify the difference between ginger soil with and without disease. Later, to investigate the influence of the well-known biocontrol agent B. velezensis and the fungicide Etridiazole on soil microbes and ginger productivity, we designed an experiment that collected the soil samples according to the different periods of ginger cultivation to examine the microbial community dynamics in the rhizome and bulk soil. We demonstrated that B. velezensis is beneficial to ginger reproduction. In accordance with our results, we suggest that B. velezensis may influence the plant’s growth by adjusting its soil microbial composition. Etridiazole, on the other hand, may have some side effects on the ginger or beneficial bacteria in the soils that inhibit ginger reproduction. IMPORTANCE Pythium myriotylum and Ralstonia solanacearum cause soft rot disease and bacterial wilt, respectively. In this study, we used the bacterial 16S V3–V4 hypervariable region of the 16S rRNA region to investigate microbe compositions in healthy and diseased ginger soil and find out the influence of the well-known biocontrol agent B. velezensis and the fungicide Etridiazole on soil microbes and ginger productivity. These results demonstrated that B. velezensis benefits ginger reproduction and may influence the soil bacterial composition, while Etridiazole may have some side effects on the ginger or beneficial bacteria in the soils. The interactions among ginger, biocontrol agents, and fungicides need to be further investigated. American Society for Microbiology 2022-09-13 /pmc/articles/PMC9603371/ /pubmed/36098526 http://dx.doi.org/10.1128/spectrum.01803-22 Text en Copyright © 2022 Wang et al. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Wang, Chih-Wei
Michelle Wong, Jing-Wen
Yeh, Shu-Shuo
Eric Hsieh, Yunli
Tseng, Ching-Hung
Yang, Shan-Hua
Tang, Sen-Lin
Soil Bacterial Community May Offer Solutions for Ginger Cultivation
title Soil Bacterial Community May Offer Solutions for Ginger Cultivation
title_full Soil Bacterial Community May Offer Solutions for Ginger Cultivation
title_fullStr Soil Bacterial Community May Offer Solutions for Ginger Cultivation
title_full_unstemmed Soil Bacterial Community May Offer Solutions for Ginger Cultivation
title_short Soil Bacterial Community May Offer Solutions for Ginger Cultivation
title_sort soil bacterial community may offer solutions for ginger cultivation
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9603371/
https://www.ncbi.nlm.nih.gov/pubmed/36098526
http://dx.doi.org/10.1128/spectrum.01803-22
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