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Magneto-Fluorescent Mesoporous Nanocarriers for the Dual-Delivery of Ofloxacin and Doxorubicin to Tackle Opportunistic Bacterial Infections in Colorectal Cancer

Cancer-related opportunistic bacterial infections are one major barrier for successful clinical therapies, often correlated to the production of genotoxic factors and higher cancer incidence. Although dual anticancer and antimicrobial therapies are a growing therapeutic fashion, they still fall shor...

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Autores principales: Marcelo, Gonçalo A., Galhano, Joana, Robalo, Tiago T., Cruz, Maria Margarida, Marcos, María D., Martínez-Máñez, Ramón, Duarte, Maria Paula, Capelo-Martínez, José Luis, Lodeiro, Carlos, Oliveira, Elisabete
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9603674/
https://www.ncbi.nlm.nih.gov/pubmed/36293142
http://dx.doi.org/10.3390/ijms232012287
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author Marcelo, Gonçalo A.
Galhano, Joana
Robalo, Tiago T.
Cruz, Maria Margarida
Marcos, María D.
Martínez-Máñez, Ramón
Duarte, Maria Paula
Capelo-Martínez, José Luis
Lodeiro, Carlos
Oliveira, Elisabete
author_facet Marcelo, Gonçalo A.
Galhano, Joana
Robalo, Tiago T.
Cruz, Maria Margarida
Marcos, María D.
Martínez-Máñez, Ramón
Duarte, Maria Paula
Capelo-Martínez, José Luis
Lodeiro, Carlos
Oliveira, Elisabete
author_sort Marcelo, Gonçalo A.
collection PubMed
description Cancer-related opportunistic bacterial infections are one major barrier for successful clinical therapies, often correlated to the production of genotoxic factors and higher cancer incidence. Although dual anticancer and antimicrobial therapies are a growing therapeutic fashion, they still fall short when it comes to specific delivery and local action in in vivo systems. Nanoparticles are seen as potential therapeutic vectors, be it by means of their intrinsic antibacterial properties and effective delivery capacity, or by means of their repeatedly reported modulation and maneuverability. Herein we report on the production of a biocompatible, antimicrobial magneto-fluorescent nanosystem (NANO3) for the delivery of a dual doxorubicin–ofloxacin formulation against cancer-related bacterial infections. The drug delivery capacity, rendered by its mesoporous silica matrix, is confirmed by the high loading capacity and stimuli-driven release of both drugs, with preference for tumor-like acidic media. The pH-dependent emission of its surface fluorescent SiQDs, provides an insight into NANO3 surface behavior and pore availability, with the SiQDs working as pore gates. Hyperthermia induces heat generation to febrile temperatures, doubling drug release. NANO3-loaded systems demonstrate significant antimicrobial activity, specifically after the application of hyperthermia conditions. NANO3 structure and antimicrobial properties confirm their potential use in a future dual anticancer and antimicrobial therapeutical vector, due to their drug loading capacity and their surface availability for further modification with bioactive, targeting species.
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spelling pubmed-96036742022-10-27 Magneto-Fluorescent Mesoporous Nanocarriers for the Dual-Delivery of Ofloxacin and Doxorubicin to Tackle Opportunistic Bacterial Infections in Colorectal Cancer Marcelo, Gonçalo A. Galhano, Joana Robalo, Tiago T. Cruz, Maria Margarida Marcos, María D. Martínez-Máñez, Ramón Duarte, Maria Paula Capelo-Martínez, José Luis Lodeiro, Carlos Oliveira, Elisabete Int J Mol Sci Article Cancer-related opportunistic bacterial infections are one major barrier for successful clinical therapies, often correlated to the production of genotoxic factors and higher cancer incidence. Although dual anticancer and antimicrobial therapies are a growing therapeutic fashion, they still fall short when it comes to specific delivery and local action in in vivo systems. Nanoparticles are seen as potential therapeutic vectors, be it by means of their intrinsic antibacterial properties and effective delivery capacity, or by means of their repeatedly reported modulation and maneuverability. Herein we report on the production of a biocompatible, antimicrobial magneto-fluorescent nanosystem (NANO3) for the delivery of a dual doxorubicin–ofloxacin formulation against cancer-related bacterial infections. The drug delivery capacity, rendered by its mesoporous silica matrix, is confirmed by the high loading capacity and stimuli-driven release of both drugs, with preference for tumor-like acidic media. The pH-dependent emission of its surface fluorescent SiQDs, provides an insight into NANO3 surface behavior and pore availability, with the SiQDs working as pore gates. Hyperthermia induces heat generation to febrile temperatures, doubling drug release. NANO3-loaded systems demonstrate significant antimicrobial activity, specifically after the application of hyperthermia conditions. NANO3 structure and antimicrobial properties confirm their potential use in a future dual anticancer and antimicrobial therapeutical vector, due to their drug loading capacity and their surface availability for further modification with bioactive, targeting species. MDPI 2022-10-14 /pmc/articles/PMC9603674/ /pubmed/36293142 http://dx.doi.org/10.3390/ijms232012287 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Marcelo, Gonçalo A.
Galhano, Joana
Robalo, Tiago T.
Cruz, Maria Margarida
Marcos, María D.
Martínez-Máñez, Ramón
Duarte, Maria Paula
Capelo-Martínez, José Luis
Lodeiro, Carlos
Oliveira, Elisabete
Magneto-Fluorescent Mesoporous Nanocarriers for the Dual-Delivery of Ofloxacin and Doxorubicin to Tackle Opportunistic Bacterial Infections in Colorectal Cancer
title Magneto-Fluorescent Mesoporous Nanocarriers for the Dual-Delivery of Ofloxacin and Doxorubicin to Tackle Opportunistic Bacterial Infections in Colorectal Cancer
title_full Magneto-Fluorescent Mesoporous Nanocarriers for the Dual-Delivery of Ofloxacin and Doxorubicin to Tackle Opportunistic Bacterial Infections in Colorectal Cancer
title_fullStr Magneto-Fluorescent Mesoporous Nanocarriers for the Dual-Delivery of Ofloxacin and Doxorubicin to Tackle Opportunistic Bacterial Infections in Colorectal Cancer
title_full_unstemmed Magneto-Fluorescent Mesoporous Nanocarriers for the Dual-Delivery of Ofloxacin and Doxorubicin to Tackle Opportunistic Bacterial Infections in Colorectal Cancer
title_short Magneto-Fluorescent Mesoporous Nanocarriers for the Dual-Delivery of Ofloxacin and Doxorubicin to Tackle Opportunistic Bacterial Infections in Colorectal Cancer
title_sort magneto-fluorescent mesoporous nanocarriers for the dual-delivery of ofloxacin and doxorubicin to tackle opportunistic bacterial infections in colorectal cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9603674/
https://www.ncbi.nlm.nih.gov/pubmed/36293142
http://dx.doi.org/10.3390/ijms232012287
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