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Relationship between Neuroglial Apoptosis and Neuroinflammation in the Epileptic Focus of the Brain and in the Blood of Patients with Drug-Resistant Epilepsy

Neuroglial apoptosis and neuroinflammation play an important role in epileptogenesis. The aim of this study is to evaluate neuronal and glial apoptosis in association with neuroinflammation in brain epileptic focus and inflammatory changes in blood in patients with focal drug-resistant epilepsy (DRE...

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Autores principales: Sokolova, Tatiana V., Zabrodskaya, Yulia M., Litovchenko, Anastasia V., Paramonova, Natalia M., Kasumov, Vugar R., Kravtsova, Svetlana V., Skiteva, Ekaterina N., Sitovskaya, Daria A., Bazhanova, Elena D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9603914/
https://www.ncbi.nlm.nih.gov/pubmed/36293411
http://dx.doi.org/10.3390/ijms232012561
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author Sokolova, Tatiana V.
Zabrodskaya, Yulia M.
Litovchenko, Anastasia V.
Paramonova, Natalia M.
Kasumov, Vugar R.
Kravtsova, Svetlana V.
Skiteva, Ekaterina N.
Sitovskaya, Daria A.
Bazhanova, Elena D.
author_facet Sokolova, Tatiana V.
Zabrodskaya, Yulia M.
Litovchenko, Anastasia V.
Paramonova, Natalia M.
Kasumov, Vugar R.
Kravtsova, Svetlana V.
Skiteva, Ekaterina N.
Sitovskaya, Daria A.
Bazhanova, Elena D.
author_sort Sokolova, Tatiana V.
collection PubMed
description Neuroglial apoptosis and neuroinflammation play an important role in epileptogenesis. The aim of this study is to evaluate neuronal and glial apoptosis in association with neuroinflammation in brain epileptic focus and inflammatory changes in blood in patients with focal drug-resistant epilepsy (DRE). Pathological changes in the temporal lobe in epilepsy (histology, transmission electron microscopy), levels of apoptotic and neuroinflammatory proteins: active caspase-3 (immunohistochemistry), full-length form caspase-3, caspase-9, FAS, FAS-L, NF-kB, TNF-α, p53 (Western blot), and cytokine levels in blood: IL-1β, IL-2, IL-4, IL-7, TNF-α, etc. (multiplex analysis) were studied. In the present work, ultrastructural and immunohistochemical apoptotic signs were found in neurons and oligodendrocytes in the temporal lobe of DRE patients. Levels of proinflammatory cytokines that play a role in apoptosis (TNF-α, FAS, NF-kB) were increased. The blood concentration of IL-4, IL-7, TNF-α is increased and IL-2 is reduced. Oligodendroglial apoptosis has been shown to play an important role in DRE pathogenesis and to explain demyelination. Thus, a comprehensive analysis of revealed changes in the blood and brain in DRE patients showed the neuroinflammation in the epileptic focus, which was combined with the development of apoptosis of glial cells and neurons. This creates conditions for the development of drug resistance and the epilepsy progression.
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spelling pubmed-96039142022-10-27 Relationship between Neuroglial Apoptosis and Neuroinflammation in the Epileptic Focus of the Brain and in the Blood of Patients with Drug-Resistant Epilepsy Sokolova, Tatiana V. Zabrodskaya, Yulia M. Litovchenko, Anastasia V. Paramonova, Natalia M. Kasumov, Vugar R. Kravtsova, Svetlana V. Skiteva, Ekaterina N. Sitovskaya, Daria A. Bazhanova, Elena D. Int J Mol Sci Article Neuroglial apoptosis and neuroinflammation play an important role in epileptogenesis. The aim of this study is to evaluate neuronal and glial apoptosis in association with neuroinflammation in brain epileptic focus and inflammatory changes in blood in patients with focal drug-resistant epilepsy (DRE). Pathological changes in the temporal lobe in epilepsy (histology, transmission electron microscopy), levels of apoptotic and neuroinflammatory proteins: active caspase-3 (immunohistochemistry), full-length form caspase-3, caspase-9, FAS, FAS-L, NF-kB, TNF-α, p53 (Western blot), and cytokine levels in blood: IL-1β, IL-2, IL-4, IL-7, TNF-α, etc. (multiplex analysis) were studied. In the present work, ultrastructural and immunohistochemical apoptotic signs were found in neurons and oligodendrocytes in the temporal lobe of DRE patients. Levels of proinflammatory cytokines that play a role in apoptosis (TNF-α, FAS, NF-kB) were increased. The blood concentration of IL-4, IL-7, TNF-α is increased and IL-2 is reduced. Oligodendroglial apoptosis has been shown to play an important role in DRE pathogenesis and to explain demyelination. Thus, a comprehensive analysis of revealed changes in the blood and brain in DRE patients showed the neuroinflammation in the epileptic focus, which was combined with the development of apoptosis of glial cells and neurons. This creates conditions for the development of drug resistance and the epilepsy progression. MDPI 2022-10-19 /pmc/articles/PMC9603914/ /pubmed/36293411 http://dx.doi.org/10.3390/ijms232012561 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sokolova, Tatiana V.
Zabrodskaya, Yulia M.
Litovchenko, Anastasia V.
Paramonova, Natalia M.
Kasumov, Vugar R.
Kravtsova, Svetlana V.
Skiteva, Ekaterina N.
Sitovskaya, Daria A.
Bazhanova, Elena D.
Relationship between Neuroglial Apoptosis and Neuroinflammation in the Epileptic Focus of the Brain and in the Blood of Patients with Drug-Resistant Epilepsy
title Relationship between Neuroglial Apoptosis and Neuroinflammation in the Epileptic Focus of the Brain and in the Blood of Patients with Drug-Resistant Epilepsy
title_full Relationship between Neuroglial Apoptosis and Neuroinflammation in the Epileptic Focus of the Brain and in the Blood of Patients with Drug-Resistant Epilepsy
title_fullStr Relationship between Neuroglial Apoptosis and Neuroinflammation in the Epileptic Focus of the Brain and in the Blood of Patients with Drug-Resistant Epilepsy
title_full_unstemmed Relationship between Neuroglial Apoptosis and Neuroinflammation in the Epileptic Focus of the Brain and in the Blood of Patients with Drug-Resistant Epilepsy
title_short Relationship between Neuroglial Apoptosis and Neuroinflammation in the Epileptic Focus of the Brain and in the Blood of Patients with Drug-Resistant Epilepsy
title_sort relationship between neuroglial apoptosis and neuroinflammation in the epileptic focus of the brain and in the blood of patients with drug-resistant epilepsy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9603914/
https://www.ncbi.nlm.nih.gov/pubmed/36293411
http://dx.doi.org/10.3390/ijms232012561
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