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Abnormal Expression of Synaptic and Extrasynaptic GABA(A) Receptor Subunits in the Dystrophin-Deficient mdx Mouse
Duchenne muscular dystrophy (DMD) is a neurodevelopmental disorder primarily caused by the loss of the full-length Dp427 dystrophin in both muscle and brain. The basis of the central comorbidities in DMD is unclear. Brain dystrophin plays a role in the clustering of central gamma-aminobutyric acid A...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604073/ https://www.ncbi.nlm.nih.gov/pubmed/36293496 http://dx.doi.org/10.3390/ijms232012617 |
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author | Zarrouki, Faouzi Goutal, Sébastien Vacca, Ophélie Garcia, Luis Tournier, Nicolas Goyenvalle, Aurélie Vaillend, Cyrille |
author_facet | Zarrouki, Faouzi Goutal, Sébastien Vacca, Ophélie Garcia, Luis Tournier, Nicolas Goyenvalle, Aurélie Vaillend, Cyrille |
author_sort | Zarrouki, Faouzi |
collection | PubMed |
description | Duchenne muscular dystrophy (DMD) is a neurodevelopmental disorder primarily caused by the loss of the full-length Dp427 dystrophin in both muscle and brain. The basis of the central comorbidities in DMD is unclear. Brain dystrophin plays a role in the clustering of central gamma-aminobutyric acid A receptors (GABA(A)Rs), and its loss in the mdx mouse alters the clustering of some synaptic subunits in central inhibitory synapses. However, the diversity of GABAergic alterations in this model is still fragmentary. In this study, the analysis of in vivo PET imaging of a benzodiazepine-binding site radioligand revealed that the global density of central GABA(A)Rs is unaffected in mdx compared with WT mice. In contrast, semi-quantitative immunoblots and immunofluorescence confocal imaging in tissue sections revealed complex and differential patterns of alterations of the expression levels and/or clustered distribution of a variety of synaptic and extrasynaptic GABA(A)R subunits in the hippocampus, cerebellum, cortex, and spinal cord. Hence, dystrophin loss not only affects the stabilization of synaptic GABA(A)Rs but also influences the subunit composition of GABA(A)Rs subtypes at both synaptic and extrasynaptic sites. This study provides new molecular outcome measures and new routes to evaluate the impact of treatments aimed at compensating alterations of the nervous system in DMD. |
format | Online Article Text |
id | pubmed-9604073 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96040732022-10-27 Abnormal Expression of Synaptic and Extrasynaptic GABA(A) Receptor Subunits in the Dystrophin-Deficient mdx Mouse Zarrouki, Faouzi Goutal, Sébastien Vacca, Ophélie Garcia, Luis Tournier, Nicolas Goyenvalle, Aurélie Vaillend, Cyrille Int J Mol Sci Article Duchenne muscular dystrophy (DMD) is a neurodevelopmental disorder primarily caused by the loss of the full-length Dp427 dystrophin in both muscle and brain. The basis of the central comorbidities in DMD is unclear. Brain dystrophin plays a role in the clustering of central gamma-aminobutyric acid A receptors (GABA(A)Rs), and its loss in the mdx mouse alters the clustering of some synaptic subunits in central inhibitory synapses. However, the diversity of GABAergic alterations in this model is still fragmentary. In this study, the analysis of in vivo PET imaging of a benzodiazepine-binding site radioligand revealed that the global density of central GABA(A)Rs is unaffected in mdx compared with WT mice. In contrast, semi-quantitative immunoblots and immunofluorescence confocal imaging in tissue sections revealed complex and differential patterns of alterations of the expression levels and/or clustered distribution of a variety of synaptic and extrasynaptic GABA(A)R subunits in the hippocampus, cerebellum, cortex, and spinal cord. Hence, dystrophin loss not only affects the stabilization of synaptic GABA(A)Rs but also influences the subunit composition of GABA(A)Rs subtypes at both synaptic and extrasynaptic sites. This study provides new molecular outcome measures and new routes to evaluate the impact of treatments aimed at compensating alterations of the nervous system in DMD. MDPI 2022-10-20 /pmc/articles/PMC9604073/ /pubmed/36293496 http://dx.doi.org/10.3390/ijms232012617 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zarrouki, Faouzi Goutal, Sébastien Vacca, Ophélie Garcia, Luis Tournier, Nicolas Goyenvalle, Aurélie Vaillend, Cyrille Abnormal Expression of Synaptic and Extrasynaptic GABA(A) Receptor Subunits in the Dystrophin-Deficient mdx Mouse |
title | Abnormal Expression of Synaptic and Extrasynaptic GABA(A) Receptor Subunits in the Dystrophin-Deficient mdx Mouse |
title_full | Abnormal Expression of Synaptic and Extrasynaptic GABA(A) Receptor Subunits in the Dystrophin-Deficient mdx Mouse |
title_fullStr | Abnormal Expression of Synaptic and Extrasynaptic GABA(A) Receptor Subunits in the Dystrophin-Deficient mdx Mouse |
title_full_unstemmed | Abnormal Expression of Synaptic and Extrasynaptic GABA(A) Receptor Subunits in the Dystrophin-Deficient mdx Mouse |
title_short | Abnormal Expression of Synaptic and Extrasynaptic GABA(A) Receptor Subunits in the Dystrophin-Deficient mdx Mouse |
title_sort | abnormal expression of synaptic and extrasynaptic gaba(a) receptor subunits in the dystrophin-deficient mdx mouse |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604073/ https://www.ncbi.nlm.nih.gov/pubmed/36293496 http://dx.doi.org/10.3390/ijms232012617 |
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