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Tolerance, Adaptation, and Cell Response Elicited by Micromonospora sp. Facing Tellurite Toxicity: A Biological and Physical-Chemical Characterization

The intense use of tellurium (Te) in industrial applications, along with the improper disposal of Te-derivatives, is causing their accumulation in the environment, where oxyanion tellurite (TeO(3)(2)(−)) is the most soluble, bioavailable, and toxic Te-species. On the other hand, tellurium is a rare...

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Autores principales: Piacenza, Elena, Campora, Simona, Carfì Pavia, Francesco, Chillura Martino, Delia Francesca, Laudicina, Vito Armando, Alduina, Rosa, Turner, Raymond Joseph, Zannoni, Davide, Presentato, Alessandro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604092/
https://www.ncbi.nlm.nih.gov/pubmed/36293484
http://dx.doi.org/10.3390/ijms232012631
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author Piacenza, Elena
Campora, Simona
Carfì Pavia, Francesco
Chillura Martino, Delia Francesca
Laudicina, Vito Armando
Alduina, Rosa
Turner, Raymond Joseph
Zannoni, Davide
Presentato, Alessandro
author_facet Piacenza, Elena
Campora, Simona
Carfì Pavia, Francesco
Chillura Martino, Delia Francesca
Laudicina, Vito Armando
Alduina, Rosa
Turner, Raymond Joseph
Zannoni, Davide
Presentato, Alessandro
author_sort Piacenza, Elena
collection PubMed
description The intense use of tellurium (Te) in industrial applications, along with the improper disposal of Te-derivatives, is causing their accumulation in the environment, where oxyanion tellurite (TeO(3)(2)(−)) is the most soluble, bioavailable, and toxic Te-species. On the other hand, tellurium is a rare metalloid element whose natural supply will end shortly with possible economic and technological effects. Thus, Te-containing waste represents the source from which Te should be recycled and recovered. Among the explored strategies, the microbial TeO(3)(2)(−) biotransformation into less toxic Te-species is the most appropriate concerning the circular economy. Actinomycetes are ideal candidates in environmental biotechnology. However, their exploration in TeO(3)(2−) biotransformation is scarce due to limited knowledge regarding oxyanion microbial processing. Here, this gap was filled by investigating the cell tolerance, adaptation, and response to TeO(3)(2−) of a Micromonospora strain isolated from a metal(loid)-rich environment. To this aim, an integrated biological, physical-chemical, and statistical approach combining physiological and biochemical assays with confocal or scanning electron (SEM) microscopy and Fourier-transform infrared spectroscopy in attenuated total reflectance mode (ATR-FTIR) was designed. Micromonospora cells exposed to TeO(3)(2−) under different physiological states revealed a series of striking cell responses, such as cell morphology changes, extracellular polymeric substance production, cell membrane damages and modifications, oxidative stress burst, protein aggregation and phosphorylation, and superoxide dismutase induction. These results highlight this Micromonospora strain as an asset for biotechnological purposes.
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spelling pubmed-96040922022-10-27 Tolerance, Adaptation, and Cell Response Elicited by Micromonospora sp. Facing Tellurite Toxicity: A Biological and Physical-Chemical Characterization Piacenza, Elena Campora, Simona Carfì Pavia, Francesco Chillura Martino, Delia Francesca Laudicina, Vito Armando Alduina, Rosa Turner, Raymond Joseph Zannoni, Davide Presentato, Alessandro Int J Mol Sci Article The intense use of tellurium (Te) in industrial applications, along with the improper disposal of Te-derivatives, is causing their accumulation in the environment, where oxyanion tellurite (TeO(3)(2)(−)) is the most soluble, bioavailable, and toxic Te-species. On the other hand, tellurium is a rare metalloid element whose natural supply will end shortly with possible economic and technological effects. Thus, Te-containing waste represents the source from which Te should be recycled and recovered. Among the explored strategies, the microbial TeO(3)(2)(−) biotransformation into less toxic Te-species is the most appropriate concerning the circular economy. Actinomycetes are ideal candidates in environmental biotechnology. However, their exploration in TeO(3)(2−) biotransformation is scarce due to limited knowledge regarding oxyanion microbial processing. Here, this gap was filled by investigating the cell tolerance, adaptation, and response to TeO(3)(2−) of a Micromonospora strain isolated from a metal(loid)-rich environment. To this aim, an integrated biological, physical-chemical, and statistical approach combining physiological and biochemical assays with confocal or scanning electron (SEM) microscopy and Fourier-transform infrared spectroscopy in attenuated total reflectance mode (ATR-FTIR) was designed. Micromonospora cells exposed to TeO(3)(2−) under different physiological states revealed a series of striking cell responses, such as cell morphology changes, extracellular polymeric substance production, cell membrane damages and modifications, oxidative stress burst, protein aggregation and phosphorylation, and superoxide dismutase induction. These results highlight this Micromonospora strain as an asset for biotechnological purposes. MDPI 2022-10-20 /pmc/articles/PMC9604092/ /pubmed/36293484 http://dx.doi.org/10.3390/ijms232012631 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Piacenza, Elena
Campora, Simona
Carfì Pavia, Francesco
Chillura Martino, Delia Francesca
Laudicina, Vito Armando
Alduina, Rosa
Turner, Raymond Joseph
Zannoni, Davide
Presentato, Alessandro
Tolerance, Adaptation, and Cell Response Elicited by Micromonospora sp. Facing Tellurite Toxicity: A Biological and Physical-Chemical Characterization
title Tolerance, Adaptation, and Cell Response Elicited by Micromonospora sp. Facing Tellurite Toxicity: A Biological and Physical-Chemical Characterization
title_full Tolerance, Adaptation, and Cell Response Elicited by Micromonospora sp. Facing Tellurite Toxicity: A Biological and Physical-Chemical Characterization
title_fullStr Tolerance, Adaptation, and Cell Response Elicited by Micromonospora sp. Facing Tellurite Toxicity: A Biological and Physical-Chemical Characterization
title_full_unstemmed Tolerance, Adaptation, and Cell Response Elicited by Micromonospora sp. Facing Tellurite Toxicity: A Biological and Physical-Chemical Characterization
title_short Tolerance, Adaptation, and Cell Response Elicited by Micromonospora sp. Facing Tellurite Toxicity: A Biological and Physical-Chemical Characterization
title_sort tolerance, adaptation, and cell response elicited by micromonospora sp. facing tellurite toxicity: a biological and physical-chemical characterization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604092/
https://www.ncbi.nlm.nih.gov/pubmed/36293484
http://dx.doi.org/10.3390/ijms232012631
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