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DNA Penetration into a Lysozyme Layer at the Surface of Aqueous Solutions

The interactions of DNA with lysozyme in the surface layer were studied by performing infrared reflection–absorption spectroscopy (IRRAS), ellipsometry, surface tensiometry, surface dilational rheology, and atomic force microscopy (AFM). A concentrated DNA solution was injected into an aqueous subph...

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Autores principales: Chirkov, Nikolay S., Lin, Shi-Yow, Michailov, Alexander V., Miller, Reinhard, Noskov, Boris A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604093/
https://www.ncbi.nlm.nih.gov/pubmed/36293234
http://dx.doi.org/10.3390/ijms232012377
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author Chirkov, Nikolay S.
Lin, Shi-Yow
Michailov, Alexander V.
Miller, Reinhard
Noskov, Boris A.
author_facet Chirkov, Nikolay S.
Lin, Shi-Yow
Michailov, Alexander V.
Miller, Reinhard
Noskov, Boris A.
author_sort Chirkov, Nikolay S.
collection PubMed
description The interactions of DNA with lysozyme in the surface layer were studied by performing infrared reflection–absorption spectroscopy (IRRAS), ellipsometry, surface tensiometry, surface dilational rheology, and atomic force microscopy (AFM). A concentrated DNA solution was injected into an aqueous subphase underneath a spread lysozyme layer. While the optical properties of the surface layer changed fast after DNA injection, the dynamic dilational surface elasticity almost did not change, thereby indicating no continuous network formation of DNA/lysozyme complexes, unlike the case of DNA interactions with a monolayer of a cationic synthetic polyelectrolyte. A relatively fast increase in optical signals after a DNA injection under a lysozyme layer indicates that DNA penetration is controlled by diffusion. At low surface pressures, the AFM images show the formation of long strands in the surface layer. Increased surface compression does not lead to the formation of a network of DNA/lysozyme aggregates as in the case of a mixed layer of DNA and synthetic polyelectrolytes, but to the appearance of some folds and ridges in the layer. The formation of more disordered aggregates is presumably a consequence of weaker interactions of lysozyme with duplex DNA and the stabilization, at the same time, of loops of unpaired nucleotides at high local lysozyme concentrations in the surface layer.
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spelling pubmed-96040932022-10-27 DNA Penetration into a Lysozyme Layer at the Surface of Aqueous Solutions Chirkov, Nikolay S. Lin, Shi-Yow Michailov, Alexander V. Miller, Reinhard Noskov, Boris A. Int J Mol Sci Article The interactions of DNA with lysozyme in the surface layer were studied by performing infrared reflection–absorption spectroscopy (IRRAS), ellipsometry, surface tensiometry, surface dilational rheology, and atomic force microscopy (AFM). A concentrated DNA solution was injected into an aqueous subphase underneath a spread lysozyme layer. While the optical properties of the surface layer changed fast after DNA injection, the dynamic dilational surface elasticity almost did not change, thereby indicating no continuous network formation of DNA/lysozyme complexes, unlike the case of DNA interactions with a monolayer of a cationic synthetic polyelectrolyte. A relatively fast increase in optical signals after a DNA injection under a lysozyme layer indicates that DNA penetration is controlled by diffusion. At low surface pressures, the AFM images show the formation of long strands in the surface layer. Increased surface compression does not lead to the formation of a network of DNA/lysozyme aggregates as in the case of a mixed layer of DNA and synthetic polyelectrolytes, but to the appearance of some folds and ridges in the layer. The formation of more disordered aggregates is presumably a consequence of weaker interactions of lysozyme with duplex DNA and the stabilization, at the same time, of loops of unpaired nucleotides at high local lysozyme concentrations in the surface layer. MDPI 2022-10-16 /pmc/articles/PMC9604093/ /pubmed/36293234 http://dx.doi.org/10.3390/ijms232012377 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chirkov, Nikolay S.
Lin, Shi-Yow
Michailov, Alexander V.
Miller, Reinhard
Noskov, Boris A.
DNA Penetration into a Lysozyme Layer at the Surface of Aqueous Solutions
title DNA Penetration into a Lysozyme Layer at the Surface of Aqueous Solutions
title_full DNA Penetration into a Lysozyme Layer at the Surface of Aqueous Solutions
title_fullStr DNA Penetration into a Lysozyme Layer at the Surface of Aqueous Solutions
title_full_unstemmed DNA Penetration into a Lysozyme Layer at the Surface of Aqueous Solutions
title_short DNA Penetration into a Lysozyme Layer at the Surface of Aqueous Solutions
title_sort dna penetration into a lysozyme layer at the surface of aqueous solutions
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604093/
https://www.ncbi.nlm.nih.gov/pubmed/36293234
http://dx.doi.org/10.3390/ijms232012377
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