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Identification of the NAC Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera

Nelumbo nucifera Gaertn. is an important perennial aquatic herb that has high ornamental, edible, medicinal, and economic value, being widely distributed and used in China. The NAC superfamily (NAM, ATAF1/2, CUC2) plays critical roles in plant growth, development, and response to abiotic and biotic...

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Autores principales: Zhao, Shuping, Jiang, Tao, Zhang, Yao, Zhang, Kailing, Feng, Kai, Wu, Peng, Li, Liangjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604248/
https://www.ncbi.nlm.nih.gov/pubmed/36293250
http://dx.doi.org/10.3390/ijms232012394
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author Zhao, Shuping
Jiang, Tao
Zhang, Yao
Zhang, Kailing
Feng, Kai
Wu, Peng
Li, Liangjun
author_facet Zhao, Shuping
Jiang, Tao
Zhang, Yao
Zhang, Kailing
Feng, Kai
Wu, Peng
Li, Liangjun
author_sort Zhao, Shuping
collection PubMed
description Nelumbo nucifera Gaertn. is an important perennial aquatic herb that has high ornamental, edible, medicinal, and economic value, being widely distributed and used in China. The NAC superfamily (NAM, ATAF1/2, CUC2) plays critical roles in plant growth, development, and response to abiotic and biotic stresses. Though there have been a few reports about NAC genes in lotus, systematic analysis is still relatively lacking. The present study aimed to characterize all the NAC genes in the lotus and obtain better insights on the NnNACs in response to salt stress by depending on ABA signaling. Here, 97 NAC genes were identified by searching the whole lotus genome based on the raw HMM models of the conserved NAM domain and NAC domain. They were characterized by bioinformatics analysis and divided into 18 subgroups based on the phylogenetic tree. Cis-element analysis demonstrated that NAC genes are responsive to biotic and abiotic stresses, light, low temperature, and plant hormones. Meanwhile, NAC genes had tissue expression specificity. qRT-PCR analysis indicated that NAC genes could be upregulated or downregulated by NaCl treatment, ABA, and fluoridone. In addition, NAC016, NAC025, and NAC070, whose encoding genes were significantly induced by NaCl and ABA, were located in the nucleus. Further analysis showed the three NAC proteins had transcriptional activation capabilities. The co-expression network analysis reflected that NAC proteins may form complexes with other proteins to play a role together. Our study provides a theoretical basis for further research to be conducted on the regulatory mechanisms of salinity resistance in the lotus.
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spelling pubmed-96042482022-10-27 Identification of the NAC Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera Zhao, Shuping Jiang, Tao Zhang, Yao Zhang, Kailing Feng, Kai Wu, Peng Li, Liangjun Int J Mol Sci Article Nelumbo nucifera Gaertn. is an important perennial aquatic herb that has high ornamental, edible, medicinal, and economic value, being widely distributed and used in China. The NAC superfamily (NAM, ATAF1/2, CUC2) plays critical roles in plant growth, development, and response to abiotic and biotic stresses. Though there have been a few reports about NAC genes in lotus, systematic analysis is still relatively lacking. The present study aimed to characterize all the NAC genes in the lotus and obtain better insights on the NnNACs in response to salt stress by depending on ABA signaling. Here, 97 NAC genes were identified by searching the whole lotus genome based on the raw HMM models of the conserved NAM domain and NAC domain. They were characterized by bioinformatics analysis and divided into 18 subgroups based on the phylogenetic tree. Cis-element analysis demonstrated that NAC genes are responsive to biotic and abiotic stresses, light, low temperature, and plant hormones. Meanwhile, NAC genes had tissue expression specificity. qRT-PCR analysis indicated that NAC genes could be upregulated or downregulated by NaCl treatment, ABA, and fluoridone. In addition, NAC016, NAC025, and NAC070, whose encoding genes were significantly induced by NaCl and ABA, were located in the nucleus. Further analysis showed the three NAC proteins had transcriptional activation capabilities. The co-expression network analysis reflected that NAC proteins may form complexes with other proteins to play a role together. Our study provides a theoretical basis for further research to be conducted on the regulatory mechanisms of salinity resistance in the lotus. MDPI 2022-10-16 /pmc/articles/PMC9604248/ /pubmed/36293250 http://dx.doi.org/10.3390/ijms232012394 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zhao, Shuping
Jiang, Tao
Zhang, Yao
Zhang, Kailing
Feng, Kai
Wu, Peng
Li, Liangjun
Identification of the NAC Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera
title Identification of the NAC Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera
title_full Identification of the NAC Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera
title_fullStr Identification of the NAC Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera
title_full_unstemmed Identification of the NAC Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera
title_short Identification of the NAC Transcription Factors and Their Function in ABA and Salinity Response in Nelumbo nucifera
title_sort identification of the nac transcription factors and their function in aba and salinity response in nelumbo nucifera
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604248/
https://www.ncbi.nlm.nih.gov/pubmed/36293250
http://dx.doi.org/10.3390/ijms232012394
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