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Aphid BCR4 Structure and Activity Uncover a New Defensin Peptide Superfamily

Aphids (Hemiptera: Aphidoidea) are among the most detrimental insects for agricultural plants, and their management is a great challenge in agronomical research. A new class of proteins, called Bacteriocyte-specific Cysteine-Rich (BCR) peptides, provides an alternative to chemical insecticides for p...

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Autores principales: Loth, Karine, Parisot, Nicolas, Paquet, Françoise, Terrasson, Hugo, Sivignon, Catherine, Rahioui, Isabelle, Ribeiro Lopes, Mélanie, Gaget, Karen, Duport, Gabrielle, Delmas, Agnès F., Aucagne, Vincent, Heddi, Abdelaziz, Calevro, Federica, da Silva, Pedro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604261/
https://www.ncbi.nlm.nih.gov/pubmed/36293341
http://dx.doi.org/10.3390/ijms232012480
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author Loth, Karine
Parisot, Nicolas
Paquet, Françoise
Terrasson, Hugo
Sivignon, Catherine
Rahioui, Isabelle
Ribeiro Lopes, Mélanie
Gaget, Karen
Duport, Gabrielle
Delmas, Agnès F.
Aucagne, Vincent
Heddi, Abdelaziz
Calevro, Federica
da Silva, Pedro
author_facet Loth, Karine
Parisot, Nicolas
Paquet, Françoise
Terrasson, Hugo
Sivignon, Catherine
Rahioui, Isabelle
Ribeiro Lopes, Mélanie
Gaget, Karen
Duport, Gabrielle
Delmas, Agnès F.
Aucagne, Vincent
Heddi, Abdelaziz
Calevro, Federica
da Silva, Pedro
author_sort Loth, Karine
collection PubMed
description Aphids (Hemiptera: Aphidoidea) are among the most detrimental insects for agricultural plants, and their management is a great challenge in agronomical research. A new class of proteins, called Bacteriocyte-specific Cysteine-Rich (BCR) peptides, provides an alternative to chemical insecticides for pest control. BCRs were initially identified in the pea aphid Acyrthosiphon pisum. They are small disulfide bond-rich proteins expressed exclusively in aphid bacteriocytes, the insect cells that host intracellular symbiotic bacteria. Here, we show that one of the A. pisum BCRs, BCR4, displays prominent insecticidal activity against the pea aphid, impairing insect survival and nymphal growth, providing evidence for its potential use as a new biopesticide. Our comparative genomics and phylogenetic analyses indicate that BCRs are restricted to the aphid lineage. The 3D structure of BCR4 reveals that this peptide belongs to an as-yet-unknown structural class of peptides and defines a new superfamily of defensins.
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spelling pubmed-96042612022-10-27 Aphid BCR4 Structure and Activity Uncover a New Defensin Peptide Superfamily Loth, Karine Parisot, Nicolas Paquet, Françoise Terrasson, Hugo Sivignon, Catherine Rahioui, Isabelle Ribeiro Lopes, Mélanie Gaget, Karen Duport, Gabrielle Delmas, Agnès F. Aucagne, Vincent Heddi, Abdelaziz Calevro, Federica da Silva, Pedro Int J Mol Sci Article Aphids (Hemiptera: Aphidoidea) are among the most detrimental insects for agricultural plants, and their management is a great challenge in agronomical research. A new class of proteins, called Bacteriocyte-specific Cysteine-Rich (BCR) peptides, provides an alternative to chemical insecticides for pest control. BCRs were initially identified in the pea aphid Acyrthosiphon pisum. They are small disulfide bond-rich proteins expressed exclusively in aphid bacteriocytes, the insect cells that host intracellular symbiotic bacteria. Here, we show that one of the A. pisum BCRs, BCR4, displays prominent insecticidal activity against the pea aphid, impairing insect survival and nymphal growth, providing evidence for its potential use as a new biopesticide. Our comparative genomics and phylogenetic analyses indicate that BCRs are restricted to the aphid lineage. The 3D structure of BCR4 reveals that this peptide belongs to an as-yet-unknown structural class of peptides and defines a new superfamily of defensins. MDPI 2022-10-18 /pmc/articles/PMC9604261/ /pubmed/36293341 http://dx.doi.org/10.3390/ijms232012480 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Loth, Karine
Parisot, Nicolas
Paquet, Françoise
Terrasson, Hugo
Sivignon, Catherine
Rahioui, Isabelle
Ribeiro Lopes, Mélanie
Gaget, Karen
Duport, Gabrielle
Delmas, Agnès F.
Aucagne, Vincent
Heddi, Abdelaziz
Calevro, Federica
da Silva, Pedro
Aphid BCR4 Structure and Activity Uncover a New Defensin Peptide Superfamily
title Aphid BCR4 Structure and Activity Uncover a New Defensin Peptide Superfamily
title_full Aphid BCR4 Structure and Activity Uncover a New Defensin Peptide Superfamily
title_fullStr Aphid BCR4 Structure and Activity Uncover a New Defensin Peptide Superfamily
title_full_unstemmed Aphid BCR4 Structure and Activity Uncover a New Defensin Peptide Superfamily
title_short Aphid BCR4 Structure and Activity Uncover a New Defensin Peptide Superfamily
title_sort aphid bcr4 structure and activity uncover a new defensin peptide superfamily
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604261/
https://www.ncbi.nlm.nih.gov/pubmed/36293341
http://dx.doi.org/10.3390/ijms232012480
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