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Annexin A2 Improves the Osteogenic Differentiation of Mesenchymal Stem Cells Exposed to High-Glucose Conditions through Lessening the Senescence

Osteoporosis is frequently found in chronic diabetic patients, and it results in an increased risk of bone fractures occurring. The underlying mechanism of osteoporosis in diabetic patients is still largely unknown. Annexin A2 (ANXA2), a family of calcium-binding proteins, has been reported to be in...

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Autores principales: Klabklai, Parin, Phetfong, Jitrada, Tangporncharoen, Rattanawan, Isarankura-Na-Ayudhya, Chartchalerm, Tawonsawatruk, Tulyapruek, Supokawej, Aungkura
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604334/
https://www.ncbi.nlm.nih.gov/pubmed/36293376
http://dx.doi.org/10.3390/ijms232012521
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author Klabklai, Parin
Phetfong, Jitrada
Tangporncharoen, Rattanawan
Isarankura-Na-Ayudhya, Chartchalerm
Tawonsawatruk, Tulyapruek
Supokawej, Aungkura
author_facet Klabklai, Parin
Phetfong, Jitrada
Tangporncharoen, Rattanawan
Isarankura-Na-Ayudhya, Chartchalerm
Tawonsawatruk, Tulyapruek
Supokawej, Aungkura
author_sort Klabklai, Parin
collection PubMed
description Osteoporosis is frequently found in chronic diabetic patients, and it results in an increased risk of bone fractures occurring. The underlying mechanism of osteoporosis in diabetic patients is still largely unknown. Annexin A2 (ANXA2), a family of calcium-binding proteins, has been reported to be involved in many biological process including bone remodeling. This study aimed to investigate the role of ANXA2 in mesenchymal stem cells (MSCs) during in vitro osteoinduction under high-glucose concentrations. Osteogenic gene expression, calcium deposition, and cellular senescence were determined. The high-glucose conditions reduced the osteogenic differentiation potential of the MSCs along with the lower expression of ANXA2. Moreover, the high-glucose conditions increased the cellular senescence of the MSCs as determined by senescence-associated β-galactosidase staining and the expression of p16, p21, and p53 genes. The addition of recombinant ANXA2 could recover the glucose-induced deterioration of the osteogenic differentiation of the MSCs and ameliorate the glucose-induced cellular senescence of the MSCs. A Western blot analysis revealed an increase in p53 and phosphorylated p53 (Ser 15), which was decreased by recombinant ANXA2 in MSC osteoblastic differentiation under high-glucose conditions. Our study suggested that the alteration of ANXA2 in high-glucose conditions may be one of the plausible factors in the deterioration of bones in diabetic patients by triggering cellular senescence.
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spelling pubmed-96043342022-10-27 Annexin A2 Improves the Osteogenic Differentiation of Mesenchymal Stem Cells Exposed to High-Glucose Conditions through Lessening the Senescence Klabklai, Parin Phetfong, Jitrada Tangporncharoen, Rattanawan Isarankura-Na-Ayudhya, Chartchalerm Tawonsawatruk, Tulyapruek Supokawej, Aungkura Int J Mol Sci Article Osteoporosis is frequently found in chronic diabetic patients, and it results in an increased risk of bone fractures occurring. The underlying mechanism of osteoporosis in diabetic patients is still largely unknown. Annexin A2 (ANXA2), a family of calcium-binding proteins, has been reported to be involved in many biological process including bone remodeling. This study aimed to investigate the role of ANXA2 in mesenchymal stem cells (MSCs) during in vitro osteoinduction under high-glucose concentrations. Osteogenic gene expression, calcium deposition, and cellular senescence were determined. The high-glucose conditions reduced the osteogenic differentiation potential of the MSCs along with the lower expression of ANXA2. Moreover, the high-glucose conditions increased the cellular senescence of the MSCs as determined by senescence-associated β-galactosidase staining and the expression of p16, p21, and p53 genes. The addition of recombinant ANXA2 could recover the glucose-induced deterioration of the osteogenic differentiation of the MSCs and ameliorate the glucose-induced cellular senescence of the MSCs. A Western blot analysis revealed an increase in p53 and phosphorylated p53 (Ser 15), which was decreased by recombinant ANXA2 in MSC osteoblastic differentiation under high-glucose conditions. Our study suggested that the alteration of ANXA2 in high-glucose conditions may be one of the plausible factors in the deterioration of bones in diabetic patients by triggering cellular senescence. MDPI 2022-10-19 /pmc/articles/PMC9604334/ /pubmed/36293376 http://dx.doi.org/10.3390/ijms232012521 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Klabklai, Parin
Phetfong, Jitrada
Tangporncharoen, Rattanawan
Isarankura-Na-Ayudhya, Chartchalerm
Tawonsawatruk, Tulyapruek
Supokawej, Aungkura
Annexin A2 Improves the Osteogenic Differentiation of Mesenchymal Stem Cells Exposed to High-Glucose Conditions through Lessening the Senescence
title Annexin A2 Improves the Osteogenic Differentiation of Mesenchymal Stem Cells Exposed to High-Glucose Conditions through Lessening the Senescence
title_full Annexin A2 Improves the Osteogenic Differentiation of Mesenchymal Stem Cells Exposed to High-Glucose Conditions through Lessening the Senescence
title_fullStr Annexin A2 Improves the Osteogenic Differentiation of Mesenchymal Stem Cells Exposed to High-Glucose Conditions through Lessening the Senescence
title_full_unstemmed Annexin A2 Improves the Osteogenic Differentiation of Mesenchymal Stem Cells Exposed to High-Glucose Conditions through Lessening the Senescence
title_short Annexin A2 Improves the Osteogenic Differentiation of Mesenchymal Stem Cells Exposed to High-Glucose Conditions through Lessening the Senescence
title_sort annexin a2 improves the osteogenic differentiation of mesenchymal stem cells exposed to high-glucose conditions through lessening the senescence
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604334/
https://www.ncbi.nlm.nih.gov/pubmed/36293376
http://dx.doi.org/10.3390/ijms232012521
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