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Mutation Hotspot for Changing the Substrate Specificity of β-N-Acetylhexosaminidase: A Library of GlcNAcases

β-N-Acetylhexosaminidase from Talaromyces flavus (TfHex; EC 3.2.1.52) is an exo-glycosidase with dual activity for cleaving N-acetylglucosamine (GlcNAc) and N-acetylgalactosamine (GalNAc) units from carbohydrates. By targeting a mutation hotspot of the active site residue Glu332, we prepared a libra...

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Detalles Bibliográficos
Autores principales:	Nekvasilová, Pavlína, Kulik, Natalia, Kotik, Michael, Petrásková, Lucie, Slámová, Kristýna, Křen, Vladimír, Bojarová, Pavla
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604439/ https://www.ncbi.nlm.nih.gov/pubmed/36293310 http://dx.doi.org/10.3390/ijms232012456

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