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A Simple and Efficient CRISPR/Cas9 System Using a Ribonucleoprotein Method for Flammulina filiformis

CRISPR/Cas9 systems were established in some edible fungi based on in vivo expressed Cas9 and guide RNA. Compared with those systems, the in vitro assembled Cas9 and sgRNA ribonucleoprotein complexes (RNPs) have more advantages, but only a few examples were reported, and the editing efficiency is re...

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Detalles Bibliográficos
Autores principales:	Liu, Jianyu, Cui, Haiyang, Wang, Ruijuan, Xu, Zhen, Yu, Hailong, Song, Chunyan, Lu, Huan, Li, Qiaozhen, Xing, Danrun, Tan, Qi, Sun, Weiming, Zou, Gen, Shang, Xiaodong
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604558/ https://www.ncbi.nlm.nih.gov/pubmed/36294565 http://dx.doi.org/10.3390/jof8101000

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