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Preparation, Anticoagulant and Antioxidant Properties of Glucosamine-Heparin Salt
Excessive inorganic ions in vivo may lead to electrolyte disorders and induce damage to the human body. Therefore, preparation of enhanced bioactivity compounds, composed of activated organic cations and organic anions, is of great interest among researchers. In this work, glucosamine-heparin salt (...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604614/ https://www.ncbi.nlm.nih.gov/pubmed/36286469 http://dx.doi.org/10.3390/md20100646 |
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author | Miao, Qin Li, Qing Tan, Wenqiang Mi, Yingqi Ma, Bing Zhang, Jingjing Guo, Zhanyong |
author_facet | Miao, Qin Li, Qing Tan, Wenqiang Mi, Yingqi Ma, Bing Zhang, Jingjing Guo, Zhanyong |
author_sort | Miao, Qin |
collection | PubMed |
description | Excessive inorganic ions in vivo may lead to electrolyte disorders and induce damage to the human body. Therefore, preparation of enhanced bioactivity compounds, composed of activated organic cations and organic anions, is of great interest among researchers. In this work, glucosamine-heparin salt (GHS) was primarily synthesized with positively charged glucosamine hydrochloride (GAH) and negatively charged heparin sodium (Heps) by ion exchange method. Then, the detailed structural information of the GHS was characterized by FTIR, (1)H NMR spectroscopy and ICP-MS. In addition, its anticoagulant potency and antioxidant properties were evaluated, respectively. The results demonstrated that GHS salt achieved enhanced antioxidant activities, including 98.78% of O(2)•(−) radical scavenging activity, 91.23% of •OH radical scavenging rate and 66.49% of DPPH radical scavenging capacity at 1.6 mg/mL, severally. Meanwhile, anticoagulant potency (ATTP) of GHS strengthened from 153.10 ± 17.14 to 180.03 ± 6.02 at 0.75 μmol/L. Thus, introducing cationic glucosamine residues into GHS could improve its anticoagulant activity. The findings suggest that GHS product with a small amount of inorganic ions can greatly abate the prime cost of antioxidants and anticoagulants, and has significant economic benefits and practical significance. |
format | Online Article Text |
id | pubmed-9604614 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96046142022-10-27 Preparation, Anticoagulant and Antioxidant Properties of Glucosamine-Heparin Salt Miao, Qin Li, Qing Tan, Wenqiang Mi, Yingqi Ma, Bing Zhang, Jingjing Guo, Zhanyong Mar Drugs Article Excessive inorganic ions in vivo may lead to electrolyte disorders and induce damage to the human body. Therefore, preparation of enhanced bioactivity compounds, composed of activated organic cations and organic anions, is of great interest among researchers. In this work, glucosamine-heparin salt (GHS) was primarily synthesized with positively charged glucosamine hydrochloride (GAH) and negatively charged heparin sodium (Heps) by ion exchange method. Then, the detailed structural information of the GHS was characterized by FTIR, (1)H NMR spectroscopy and ICP-MS. In addition, its anticoagulant potency and antioxidant properties were evaluated, respectively. The results demonstrated that GHS salt achieved enhanced antioxidant activities, including 98.78% of O(2)•(−) radical scavenging activity, 91.23% of •OH radical scavenging rate and 66.49% of DPPH radical scavenging capacity at 1.6 mg/mL, severally. Meanwhile, anticoagulant potency (ATTP) of GHS strengthened from 153.10 ± 17.14 to 180.03 ± 6.02 at 0.75 μmol/L. Thus, introducing cationic glucosamine residues into GHS could improve its anticoagulant activity. The findings suggest that GHS product with a small amount of inorganic ions can greatly abate the prime cost of antioxidants and anticoagulants, and has significant economic benefits and practical significance. MDPI 2022-10-18 /pmc/articles/PMC9604614/ /pubmed/36286469 http://dx.doi.org/10.3390/md20100646 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Miao, Qin Li, Qing Tan, Wenqiang Mi, Yingqi Ma, Bing Zhang, Jingjing Guo, Zhanyong Preparation, Anticoagulant and Antioxidant Properties of Glucosamine-Heparin Salt |
title | Preparation, Anticoagulant and Antioxidant Properties of Glucosamine-Heparin Salt |
title_full | Preparation, Anticoagulant and Antioxidant Properties of Glucosamine-Heparin Salt |
title_fullStr | Preparation, Anticoagulant and Antioxidant Properties of Glucosamine-Heparin Salt |
title_full_unstemmed | Preparation, Anticoagulant and Antioxidant Properties of Glucosamine-Heparin Salt |
title_short | Preparation, Anticoagulant and Antioxidant Properties of Glucosamine-Heparin Salt |
title_sort | preparation, anticoagulant and antioxidant properties of glucosamine-heparin salt |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604614/ https://www.ncbi.nlm.nih.gov/pubmed/36286469 http://dx.doi.org/10.3390/md20100646 |
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