Cargando…

Anti-Inflammatory and Antioxidant Properties of Malapterurus electricus Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects

The protective and therapeutic anti-inflammatory and antioxidant potency of Malapterurus electricus (F. Malapteruridae) skin fish methanolic extract (FE) (300 mg/kg.b.wt/day for 7 days, orally) was tested in monosodium urate(MSU)-induced arthritic Wistar albino male rats’ joints. Serum uric acid, TN...

Descripción completa

Detalles Bibliográficos
Autores principales: Elmaidomy, Abeer H., Mohamed, Esraa M., Aly, Hanan F., Younis, Eman A., Shams, Shams Gamal Eldin, Altemani, Faisal H., Alzubaidi, Mubarak A., Almaghrabi, Mohammed, Harbi, Adnan Al, Alsenani, Faisal, Sayed, Ahmed M., Abdelmohsen, Usama Ramadan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604635/
https://www.ncbi.nlm.nih.gov/pubmed/36286462
http://dx.doi.org/10.3390/md20100639
_version_ 1784817863709687808
author Elmaidomy, Abeer H.
Mohamed, Esraa M.
Aly, Hanan F.
Younis, Eman A.
Shams, Shams Gamal Eldin
Altemani, Faisal H.
Alzubaidi, Mubarak A.
Almaghrabi, Mohammed
Harbi, Adnan Al
Alsenani, Faisal
Sayed, Ahmed M.
Abdelmohsen, Usama Ramadan
author_facet Elmaidomy, Abeer H.
Mohamed, Esraa M.
Aly, Hanan F.
Younis, Eman A.
Shams, Shams Gamal Eldin
Altemani, Faisal H.
Alzubaidi, Mubarak A.
Almaghrabi, Mohammed
Harbi, Adnan Al
Alsenani, Faisal
Sayed, Ahmed M.
Abdelmohsen, Usama Ramadan
author_sort Elmaidomy, Abeer H.
collection PubMed
description The protective and therapeutic anti-inflammatory and antioxidant potency of Malapterurus electricus (F. Malapteruridae) skin fish methanolic extract (FE) (300 mg/kg.b.wt/day for 7 days, orally) was tested in monosodium urate(MSU)-induced arthritic Wistar albino male rats’ joints. Serum uric acid, TNF-α, IL-1β, NF-𝜅B, MDA, GSH, catalase, SOD, and glutathione reductase levels were all measured. According to the findings, FE significantly reduced uric acid levels and ankle swelling in both protective and therapeutic groups. Furthermore, it has anti-inflammatory effects by downregulating inflammatory cytokines, primarily through decreased oxidative stress and increased antioxidant status. All the aforementioned lesions were significantly improved in protected and treated rats with FE, according to histopathological findings. iNOS immunostaining revealed that protected and treated arthritic rats with FE had weak positive immune-reactive cells. Phytochemical analysis revealed that FE was high in fatty and amino acids. The most abundant compounds were vaccenic (24.52%), 9-octadecenoic (11.66%), palmitic (34.66%), stearic acids (14.63%), glycine (0.813 mg/100 mg), and alanine (1.645 mg/100 mg). Extensive molecular modelling and dynamics simulation experiments revealed that compound 4 has the potential to target and inhibit COX isoforms with a higher affinity for COX-2. As a result, we contend that FE could be a promising protective and therapeutic option for arthritis, aiding in the prevention and progression of this chronic inflammatory disease.
format Online
Article
Text
id pubmed-9604635
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96046352022-10-27 Anti-Inflammatory and Antioxidant Properties of Malapterurus electricus Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects Elmaidomy, Abeer H. Mohamed, Esraa M. Aly, Hanan F. Younis, Eman A. Shams, Shams Gamal Eldin Altemani, Faisal H. Alzubaidi, Mubarak A. Almaghrabi, Mohammed Harbi, Adnan Al Alsenani, Faisal Sayed, Ahmed M. Abdelmohsen, Usama Ramadan Mar Drugs Article The protective and therapeutic anti-inflammatory and antioxidant potency of Malapterurus electricus (F. Malapteruridae) skin fish methanolic extract (FE) (300 mg/kg.b.wt/day for 7 days, orally) was tested in monosodium urate(MSU)-induced arthritic Wistar albino male rats’ joints. Serum uric acid, TNF-α, IL-1β, NF-𝜅B, MDA, GSH, catalase, SOD, and glutathione reductase levels were all measured. According to the findings, FE significantly reduced uric acid levels and ankle swelling in both protective and therapeutic groups. Furthermore, it has anti-inflammatory effects by downregulating inflammatory cytokines, primarily through decreased oxidative stress and increased antioxidant status. All the aforementioned lesions were significantly improved in protected and treated rats with FE, according to histopathological findings. iNOS immunostaining revealed that protected and treated arthritic rats with FE had weak positive immune-reactive cells. Phytochemical analysis revealed that FE was high in fatty and amino acids. The most abundant compounds were vaccenic (24.52%), 9-octadecenoic (11.66%), palmitic (34.66%), stearic acids (14.63%), glycine (0.813 mg/100 mg), and alanine (1.645 mg/100 mg). Extensive molecular modelling and dynamics simulation experiments revealed that compound 4 has the potential to target and inhibit COX isoforms with a higher affinity for COX-2. As a result, we contend that FE could be a promising protective and therapeutic option for arthritis, aiding in the prevention and progression of this chronic inflammatory disease. MDPI 2022-10-14 /pmc/articles/PMC9604635/ /pubmed/36286462 http://dx.doi.org/10.3390/md20100639 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Elmaidomy, Abeer H.
Mohamed, Esraa M.
Aly, Hanan F.
Younis, Eman A.
Shams, Shams Gamal Eldin
Altemani, Faisal H.
Alzubaidi, Mubarak A.
Almaghrabi, Mohammed
Harbi, Adnan Al
Alsenani, Faisal
Sayed, Ahmed M.
Abdelmohsen, Usama Ramadan
Anti-Inflammatory and Antioxidant Properties of Malapterurus electricus Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects
title Anti-Inflammatory and Antioxidant Properties of Malapterurus electricus Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects
title_full Anti-Inflammatory and Antioxidant Properties of Malapterurus electricus Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects
title_fullStr Anti-Inflammatory and Antioxidant Properties of Malapterurus electricus Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects
title_full_unstemmed Anti-Inflammatory and Antioxidant Properties of Malapterurus electricus Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects
title_short Anti-Inflammatory and Antioxidant Properties of Malapterurus electricus Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects
title_sort anti-inflammatory and antioxidant properties of malapterurus electricus skin fish methanolic extract in arthritic rats: therapeutic and protective effects
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604635/
https://www.ncbi.nlm.nih.gov/pubmed/36286462
http://dx.doi.org/10.3390/md20100639
work_keys_str_mv AT elmaidomyabeerh antiinflammatoryandantioxidantpropertiesofmalapteruruselectricusskinfishmethanolicextractinarthriticratstherapeuticandprotectiveeffects
AT mohamedesraam antiinflammatoryandantioxidantpropertiesofmalapteruruselectricusskinfishmethanolicextractinarthriticratstherapeuticandprotectiveeffects
AT alyhananf antiinflammatoryandantioxidantpropertiesofmalapteruruselectricusskinfishmethanolicextractinarthriticratstherapeuticandprotectiveeffects
AT younisemana antiinflammatoryandantioxidantpropertiesofmalapteruruselectricusskinfishmethanolicextractinarthriticratstherapeuticandprotectiveeffects
AT shamsshamsgamaleldin antiinflammatoryandantioxidantpropertiesofmalapteruruselectricusskinfishmethanolicextractinarthriticratstherapeuticandprotectiveeffects
AT altemanifaisalh antiinflammatoryandantioxidantpropertiesofmalapteruruselectricusskinfishmethanolicextractinarthriticratstherapeuticandprotectiveeffects
AT alzubaidimubaraka antiinflammatoryandantioxidantpropertiesofmalapteruruselectricusskinfishmethanolicextractinarthriticratstherapeuticandprotectiveeffects
AT almaghrabimohammed antiinflammatoryandantioxidantpropertiesofmalapteruruselectricusskinfishmethanolicextractinarthriticratstherapeuticandprotectiveeffects
AT harbiadnanal antiinflammatoryandantioxidantpropertiesofmalapteruruselectricusskinfishmethanolicextractinarthriticratstherapeuticandprotectiveeffects
AT alsenanifaisal antiinflammatoryandantioxidantpropertiesofmalapteruruselectricusskinfishmethanolicextractinarthriticratstherapeuticandprotectiveeffects
AT sayedahmedm antiinflammatoryandantioxidantpropertiesofmalapteruruselectricusskinfishmethanolicextractinarthriticratstherapeuticandprotectiveeffects
AT abdelmohsenusamaramadan antiinflammatoryandantioxidantpropertiesofmalapteruruselectricusskinfishmethanolicextractinarthriticratstherapeuticandprotectiveeffects