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Anti-Inflammatory and Antioxidant Properties of Malapterurus electricus Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects
The protective and therapeutic anti-inflammatory and antioxidant potency of Malapterurus electricus (F. Malapteruridae) skin fish methanolic extract (FE) (300 mg/kg.b.wt/day for 7 days, orally) was tested in monosodium urate(MSU)-induced arthritic Wistar albino male rats’ joints. Serum uric acid, TN...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604635/ https://www.ncbi.nlm.nih.gov/pubmed/36286462 http://dx.doi.org/10.3390/md20100639 |
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author | Elmaidomy, Abeer H. Mohamed, Esraa M. Aly, Hanan F. Younis, Eman A. Shams, Shams Gamal Eldin Altemani, Faisal H. Alzubaidi, Mubarak A. Almaghrabi, Mohammed Harbi, Adnan Al Alsenani, Faisal Sayed, Ahmed M. Abdelmohsen, Usama Ramadan |
author_facet | Elmaidomy, Abeer H. Mohamed, Esraa M. Aly, Hanan F. Younis, Eman A. Shams, Shams Gamal Eldin Altemani, Faisal H. Alzubaidi, Mubarak A. Almaghrabi, Mohammed Harbi, Adnan Al Alsenani, Faisal Sayed, Ahmed M. Abdelmohsen, Usama Ramadan |
author_sort | Elmaidomy, Abeer H. |
collection | PubMed |
description | The protective and therapeutic anti-inflammatory and antioxidant potency of Malapterurus electricus (F. Malapteruridae) skin fish methanolic extract (FE) (300 mg/kg.b.wt/day for 7 days, orally) was tested in monosodium urate(MSU)-induced arthritic Wistar albino male rats’ joints. Serum uric acid, TNF-α, IL-1β, NF-𝜅B, MDA, GSH, catalase, SOD, and glutathione reductase levels were all measured. According to the findings, FE significantly reduced uric acid levels and ankle swelling in both protective and therapeutic groups. Furthermore, it has anti-inflammatory effects by downregulating inflammatory cytokines, primarily through decreased oxidative stress and increased antioxidant status. All the aforementioned lesions were significantly improved in protected and treated rats with FE, according to histopathological findings. iNOS immunostaining revealed that protected and treated arthritic rats with FE had weak positive immune-reactive cells. Phytochemical analysis revealed that FE was high in fatty and amino acids. The most abundant compounds were vaccenic (24.52%), 9-octadecenoic (11.66%), palmitic (34.66%), stearic acids (14.63%), glycine (0.813 mg/100 mg), and alanine (1.645 mg/100 mg). Extensive molecular modelling and dynamics simulation experiments revealed that compound 4 has the potential to target and inhibit COX isoforms with a higher affinity for COX-2. As a result, we contend that FE could be a promising protective and therapeutic option for arthritis, aiding in the prevention and progression of this chronic inflammatory disease. |
format | Online Article Text |
id | pubmed-9604635 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96046352022-10-27 Anti-Inflammatory and Antioxidant Properties of Malapterurus electricus Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects Elmaidomy, Abeer H. Mohamed, Esraa M. Aly, Hanan F. Younis, Eman A. Shams, Shams Gamal Eldin Altemani, Faisal H. Alzubaidi, Mubarak A. Almaghrabi, Mohammed Harbi, Adnan Al Alsenani, Faisal Sayed, Ahmed M. Abdelmohsen, Usama Ramadan Mar Drugs Article The protective and therapeutic anti-inflammatory and antioxidant potency of Malapterurus electricus (F. Malapteruridae) skin fish methanolic extract (FE) (300 mg/kg.b.wt/day for 7 days, orally) was tested in monosodium urate(MSU)-induced arthritic Wistar albino male rats’ joints. Serum uric acid, TNF-α, IL-1β, NF-𝜅B, MDA, GSH, catalase, SOD, and glutathione reductase levels were all measured. According to the findings, FE significantly reduced uric acid levels and ankle swelling in both protective and therapeutic groups. Furthermore, it has anti-inflammatory effects by downregulating inflammatory cytokines, primarily through decreased oxidative stress and increased antioxidant status. All the aforementioned lesions were significantly improved in protected and treated rats with FE, according to histopathological findings. iNOS immunostaining revealed that protected and treated arthritic rats with FE had weak positive immune-reactive cells. Phytochemical analysis revealed that FE was high in fatty and amino acids. The most abundant compounds were vaccenic (24.52%), 9-octadecenoic (11.66%), palmitic (34.66%), stearic acids (14.63%), glycine (0.813 mg/100 mg), and alanine (1.645 mg/100 mg). Extensive molecular modelling and dynamics simulation experiments revealed that compound 4 has the potential to target and inhibit COX isoforms with a higher affinity for COX-2. As a result, we contend that FE could be a promising protective and therapeutic option for arthritis, aiding in the prevention and progression of this chronic inflammatory disease. MDPI 2022-10-14 /pmc/articles/PMC9604635/ /pubmed/36286462 http://dx.doi.org/10.3390/md20100639 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Elmaidomy, Abeer H. Mohamed, Esraa M. Aly, Hanan F. Younis, Eman A. Shams, Shams Gamal Eldin Altemani, Faisal H. Alzubaidi, Mubarak A. Almaghrabi, Mohammed Harbi, Adnan Al Alsenani, Faisal Sayed, Ahmed M. Abdelmohsen, Usama Ramadan Anti-Inflammatory and Antioxidant Properties of Malapterurus electricus Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects |
title | Anti-Inflammatory and Antioxidant Properties of Malapterurus electricus Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects |
title_full | Anti-Inflammatory and Antioxidant Properties of Malapterurus electricus Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects |
title_fullStr | Anti-Inflammatory and Antioxidant Properties of Malapterurus electricus Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects |
title_full_unstemmed | Anti-Inflammatory and Antioxidant Properties of Malapterurus electricus Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects |
title_short | Anti-Inflammatory and Antioxidant Properties of Malapterurus electricus Skin Fish Methanolic Extract in Arthritic Rats: Therapeutic and Protective Effects |
title_sort | anti-inflammatory and antioxidant properties of malapterurus electricus skin fish methanolic extract in arthritic rats: therapeutic and protective effects |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604635/ https://www.ncbi.nlm.nih.gov/pubmed/36286462 http://dx.doi.org/10.3390/md20100639 |
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