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Morphological and Molecular Characterization of Calonectria foliicola Associated with Leaf Blight on Rubber Tree (Hevea brasiliensis) in Thailand
Leaf blight is commonly observed in rubber trees (Hevea brasiliensis) and can be caused by several fungal species. From October to December 2021, the emergence rubber tree disease was observed in Krabi province, southern Thailand. Small brown to dark brown spots developed on the leaves of rubber tre...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604915/ https://www.ncbi.nlm.nih.gov/pubmed/36294551 http://dx.doi.org/10.3390/jof8100986 |
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author | Thaochan, Narit Pornsuriya, Chaninun Chairin, Thanunchanok Chomnunti, Putarak Sunpapao, Anurag |
author_facet | Thaochan, Narit Pornsuriya, Chaninun Chairin, Thanunchanok Chomnunti, Putarak Sunpapao, Anurag |
author_sort | Thaochan, Narit |
collection | PubMed |
description | Leaf blight is commonly observed in rubber trees (Hevea brasiliensis) and can be caused by several fungal species. From October to December 2021, the emergence rubber tree disease was observed in Krabi province, southern Thailand. Small brown to dark brown spots developed on the leaves of rubber trees and later expanded into most parts of the leaves. Fungal isolates were isolated from infected tissues and a total of 15 Calonectria-like isolates were recovered from 10 infected leaf samples. Pathogenicity testing using the agar plug method revealed that four isolates caused leaf blight on rubber tree, similar to the situation in natural infections. Based on morphological study and the molecular properties of internal transcribed spacer (ITS), calmodulin (cal), translation elongation factor 1-α (tef1-α), and β-tubulin 2 (tub2) sequences, the four fungal isolates were identified as Calonectria foliicola. To the best of our knowledge, this is the first report of rubber trees pas a new host for C. foliicola in Thailand and elsewhere. This study reports on an emerging disease affecting rubber trees in Thailand, and the results are of benefit for the development of an appropriate method to manage this emerging disease in Thailand. |
format | Online Article Text |
id | pubmed-9604915 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96049152022-10-27 Morphological and Molecular Characterization of Calonectria foliicola Associated with Leaf Blight on Rubber Tree (Hevea brasiliensis) in Thailand Thaochan, Narit Pornsuriya, Chaninun Chairin, Thanunchanok Chomnunti, Putarak Sunpapao, Anurag J Fungi (Basel) Article Leaf blight is commonly observed in rubber trees (Hevea brasiliensis) and can be caused by several fungal species. From October to December 2021, the emergence rubber tree disease was observed in Krabi province, southern Thailand. Small brown to dark brown spots developed on the leaves of rubber trees and later expanded into most parts of the leaves. Fungal isolates were isolated from infected tissues and a total of 15 Calonectria-like isolates were recovered from 10 infected leaf samples. Pathogenicity testing using the agar plug method revealed that four isolates caused leaf blight on rubber tree, similar to the situation in natural infections. Based on morphological study and the molecular properties of internal transcribed spacer (ITS), calmodulin (cal), translation elongation factor 1-α (tef1-α), and β-tubulin 2 (tub2) sequences, the four fungal isolates were identified as Calonectria foliicola. To the best of our knowledge, this is the first report of rubber trees pas a new host for C. foliicola in Thailand and elsewhere. This study reports on an emerging disease affecting rubber trees in Thailand, and the results are of benefit for the development of an appropriate method to manage this emerging disease in Thailand. MDPI 2022-09-20 /pmc/articles/PMC9604915/ /pubmed/36294551 http://dx.doi.org/10.3390/jof8100986 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Thaochan, Narit Pornsuriya, Chaninun Chairin, Thanunchanok Chomnunti, Putarak Sunpapao, Anurag Morphological and Molecular Characterization of Calonectria foliicola Associated with Leaf Blight on Rubber Tree (Hevea brasiliensis) in Thailand |
title | Morphological and Molecular Characterization of Calonectria foliicola Associated with Leaf Blight on Rubber Tree (Hevea brasiliensis) in Thailand |
title_full | Morphological and Molecular Characterization of Calonectria foliicola Associated with Leaf Blight on Rubber Tree (Hevea brasiliensis) in Thailand |
title_fullStr | Morphological and Molecular Characterization of Calonectria foliicola Associated with Leaf Blight on Rubber Tree (Hevea brasiliensis) in Thailand |
title_full_unstemmed | Morphological and Molecular Characterization of Calonectria foliicola Associated with Leaf Blight on Rubber Tree (Hevea brasiliensis) in Thailand |
title_short | Morphological and Molecular Characterization of Calonectria foliicola Associated with Leaf Blight on Rubber Tree (Hevea brasiliensis) in Thailand |
title_sort | morphological and molecular characterization of calonectria foliicola associated with leaf blight on rubber tree (hevea brasiliensis) in thailand |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9604915/ https://www.ncbi.nlm.nih.gov/pubmed/36294551 http://dx.doi.org/10.3390/jof8100986 |
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