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Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us?

Multidrug-resistant bacteria are of critical importance and a problem for human health and food preservation; the discovery of new antimicrobial substances to control their proliferation is part of the solution. This work reports on 57 antagonistic Aeromonas strains, of which 38 strains were antagon...

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Autores principales: da Silva, Sheila, Guedes, Fernanda Alves de Freitas, Amaral, João Ricardo Vidal, Ribeiro, José Roberto de Assis, de Souza, Yuri Pinheiro Alves, de Freitas-Almeida, Ângela Correa, Thompson, Fabiano Lopes, Ramos, Rommel Thiago Jucá, Whiteley, Andrew Steven, Macrae, Andrew, de Oliveira, Selma Soares
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9605075/
https://www.ncbi.nlm.nih.gov/pubmed/36294926
http://dx.doi.org/10.3390/life12101492
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author da Silva, Sheila
Guedes, Fernanda Alves de Freitas
Amaral, João Ricardo Vidal
Ribeiro, José Roberto de Assis
de Souza, Yuri Pinheiro Alves
de Freitas-Almeida, Ângela Correa
Thompson, Fabiano Lopes
Ramos, Rommel Thiago Jucá
Whiteley, Andrew Steven
Macrae, Andrew
de Oliveira, Selma Soares
author_facet da Silva, Sheila
Guedes, Fernanda Alves de Freitas
Amaral, João Ricardo Vidal
Ribeiro, José Roberto de Assis
de Souza, Yuri Pinheiro Alves
de Freitas-Almeida, Ângela Correa
Thompson, Fabiano Lopes
Ramos, Rommel Thiago Jucá
Whiteley, Andrew Steven
Macrae, Andrew
de Oliveira, Selma Soares
author_sort da Silva, Sheila
collection PubMed
description Multidrug-resistant bacteria are of critical importance and a problem for human health and food preservation; the discovery of new antimicrobial substances to control their proliferation is part of the solution. This work reports on 57 antagonistic Aeromonas strains, of which 38 strains were antagonistic towards problematic human pathogens. The genome of the most antagonistic strain was sequenced and identified as Aeromonas allosaccharophila. Its genome was fully annotated and mined for genes that might explain that activity. Strain AE59-TE was antagonistic toward clinically relevant gram-negative and gram-positive multidrug-resistant bacteria, including Klebsiella pneumoniae KPC, Escherichia coli ESBL, Salmonella typhimurium, and Staphylococcus aureus MRSA. Strain AE59-TE2 was identified by multilocus sequence analysis. Genome mining identified four genes homologous to the bacteriocin, zoocin A from Streptococcus equi and a gene 98% similar to cvpA linked to colicin V production. A. allosaccharophila strain AE59-TE2 produced antimicrobial activity against a broad range of bacteria, including important gram-negative bacteria, not typically targeted by bacteriocins. Herewere described novel zoocin genes that are promising for industrial applications in the food and health sectors. Interesting and important antagonistic activity is described combined with the first detailed genomic analysis of the species Aeromonas allosaccharophila.
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spelling pubmed-96050752022-10-27 Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us? da Silva, Sheila Guedes, Fernanda Alves de Freitas Amaral, João Ricardo Vidal Ribeiro, José Roberto de Assis de Souza, Yuri Pinheiro Alves de Freitas-Almeida, Ângela Correa Thompson, Fabiano Lopes Ramos, Rommel Thiago Jucá Whiteley, Andrew Steven Macrae, Andrew de Oliveira, Selma Soares Life (Basel) Article Multidrug-resistant bacteria are of critical importance and a problem for human health and food preservation; the discovery of new antimicrobial substances to control their proliferation is part of the solution. This work reports on 57 antagonistic Aeromonas strains, of which 38 strains were antagonistic towards problematic human pathogens. The genome of the most antagonistic strain was sequenced and identified as Aeromonas allosaccharophila. Its genome was fully annotated and mined for genes that might explain that activity. Strain AE59-TE was antagonistic toward clinically relevant gram-negative and gram-positive multidrug-resistant bacteria, including Klebsiella pneumoniae KPC, Escherichia coli ESBL, Salmonella typhimurium, and Staphylococcus aureus MRSA. Strain AE59-TE2 was identified by multilocus sequence analysis. Genome mining identified four genes homologous to the bacteriocin, zoocin A from Streptococcus equi and a gene 98% similar to cvpA linked to colicin V production. A. allosaccharophila strain AE59-TE2 produced antimicrobial activity against a broad range of bacteria, including important gram-negative bacteria, not typically targeted by bacteriocins. Herewere described novel zoocin genes that are promising for industrial applications in the food and health sectors. Interesting and important antagonistic activity is described combined with the first detailed genomic analysis of the species Aeromonas allosaccharophila. MDPI 2022-09-26 /pmc/articles/PMC9605075/ /pubmed/36294926 http://dx.doi.org/10.3390/life12101492 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
da Silva, Sheila
Guedes, Fernanda Alves de Freitas
Amaral, João Ricardo Vidal
Ribeiro, José Roberto de Assis
de Souza, Yuri Pinheiro Alves
de Freitas-Almeida, Ângela Correa
Thompson, Fabiano Lopes
Ramos, Rommel Thiago Jucá
Whiteley, Andrew Steven
Macrae, Andrew
de Oliveira, Selma Soares
Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us?
title Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us?
title_full Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us?
title_fullStr Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us?
title_full_unstemmed Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us?
title_short Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us?
title_sort aeromonas allosaccharophila strain ae59-te2 is highly antagonistic towards multidrug-resistant human pathogens, what does its genome tell us?
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9605075/
https://www.ncbi.nlm.nih.gov/pubmed/36294926
http://dx.doi.org/10.3390/life12101492
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