Cargando…
Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us?
Multidrug-resistant bacteria are of critical importance and a problem for human health and food preservation; the discovery of new antimicrobial substances to control their proliferation is part of the solution. This work reports on 57 antagonistic Aeromonas strains, of which 38 strains were antagon...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9605075/ https://www.ncbi.nlm.nih.gov/pubmed/36294926 http://dx.doi.org/10.3390/life12101492 |
_version_ | 1784817975441752064 |
---|---|
author | da Silva, Sheila Guedes, Fernanda Alves de Freitas Amaral, João Ricardo Vidal Ribeiro, José Roberto de Assis de Souza, Yuri Pinheiro Alves de Freitas-Almeida, Ângela Correa Thompson, Fabiano Lopes Ramos, Rommel Thiago Jucá Whiteley, Andrew Steven Macrae, Andrew de Oliveira, Selma Soares |
author_facet | da Silva, Sheila Guedes, Fernanda Alves de Freitas Amaral, João Ricardo Vidal Ribeiro, José Roberto de Assis de Souza, Yuri Pinheiro Alves de Freitas-Almeida, Ângela Correa Thompson, Fabiano Lopes Ramos, Rommel Thiago Jucá Whiteley, Andrew Steven Macrae, Andrew de Oliveira, Selma Soares |
author_sort | da Silva, Sheila |
collection | PubMed |
description | Multidrug-resistant bacteria are of critical importance and a problem for human health and food preservation; the discovery of new antimicrobial substances to control their proliferation is part of the solution. This work reports on 57 antagonistic Aeromonas strains, of which 38 strains were antagonistic towards problematic human pathogens. The genome of the most antagonistic strain was sequenced and identified as Aeromonas allosaccharophila. Its genome was fully annotated and mined for genes that might explain that activity. Strain AE59-TE was antagonistic toward clinically relevant gram-negative and gram-positive multidrug-resistant bacteria, including Klebsiella pneumoniae KPC, Escherichia coli ESBL, Salmonella typhimurium, and Staphylococcus aureus MRSA. Strain AE59-TE2 was identified by multilocus sequence analysis. Genome mining identified four genes homologous to the bacteriocin, zoocin A from Streptococcus equi and a gene 98% similar to cvpA linked to colicin V production. A. allosaccharophila strain AE59-TE2 produced antimicrobial activity against a broad range of bacteria, including important gram-negative bacteria, not typically targeted by bacteriocins. Herewere described novel zoocin genes that are promising for industrial applications in the food and health sectors. Interesting and important antagonistic activity is described combined with the first detailed genomic analysis of the species Aeromonas allosaccharophila. |
format | Online Article Text |
id | pubmed-9605075 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96050752022-10-27 Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us? da Silva, Sheila Guedes, Fernanda Alves de Freitas Amaral, João Ricardo Vidal Ribeiro, José Roberto de Assis de Souza, Yuri Pinheiro Alves de Freitas-Almeida, Ângela Correa Thompson, Fabiano Lopes Ramos, Rommel Thiago Jucá Whiteley, Andrew Steven Macrae, Andrew de Oliveira, Selma Soares Life (Basel) Article Multidrug-resistant bacteria are of critical importance and a problem for human health and food preservation; the discovery of new antimicrobial substances to control their proliferation is part of the solution. This work reports on 57 antagonistic Aeromonas strains, of which 38 strains were antagonistic towards problematic human pathogens. The genome of the most antagonistic strain was sequenced and identified as Aeromonas allosaccharophila. Its genome was fully annotated and mined for genes that might explain that activity. Strain AE59-TE was antagonistic toward clinically relevant gram-negative and gram-positive multidrug-resistant bacteria, including Klebsiella pneumoniae KPC, Escherichia coli ESBL, Salmonella typhimurium, and Staphylococcus aureus MRSA. Strain AE59-TE2 was identified by multilocus sequence analysis. Genome mining identified four genes homologous to the bacteriocin, zoocin A from Streptococcus equi and a gene 98% similar to cvpA linked to colicin V production. A. allosaccharophila strain AE59-TE2 produced antimicrobial activity against a broad range of bacteria, including important gram-negative bacteria, not typically targeted by bacteriocins. Herewere described novel zoocin genes that are promising for industrial applications in the food and health sectors. Interesting and important antagonistic activity is described combined with the first detailed genomic analysis of the species Aeromonas allosaccharophila. MDPI 2022-09-26 /pmc/articles/PMC9605075/ /pubmed/36294926 http://dx.doi.org/10.3390/life12101492 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article da Silva, Sheila Guedes, Fernanda Alves de Freitas Amaral, João Ricardo Vidal Ribeiro, José Roberto de Assis de Souza, Yuri Pinheiro Alves de Freitas-Almeida, Ângela Correa Thompson, Fabiano Lopes Ramos, Rommel Thiago Jucá Whiteley, Andrew Steven Macrae, Andrew de Oliveira, Selma Soares Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us? |
title | Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us? |
title_full | Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us? |
title_fullStr | Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us? |
title_full_unstemmed | Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us? |
title_short | Aeromonas allosaccharophila Strain AE59-TE2 Is Highly Antagonistic towards Multidrug-Resistant Human Pathogens, What Does Its Genome Tell Us? |
title_sort | aeromonas allosaccharophila strain ae59-te2 is highly antagonistic towards multidrug-resistant human pathogens, what does its genome tell us? |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9605075/ https://www.ncbi.nlm.nih.gov/pubmed/36294926 http://dx.doi.org/10.3390/life12101492 |
work_keys_str_mv | AT dasilvasheila aeromonasallosaccharophilastrainae59te2ishighlyantagonistictowardsmultidrugresistanthumanpathogenswhatdoesitsgenometellus AT guedesfernandaalvesdefreitas aeromonasallosaccharophilastrainae59te2ishighlyantagonistictowardsmultidrugresistanthumanpathogenswhatdoesitsgenometellus AT amaraljoaoricardovidal aeromonasallosaccharophilastrainae59te2ishighlyantagonistictowardsmultidrugresistanthumanpathogenswhatdoesitsgenometellus AT ribeirojoserobertodeassis aeromonasallosaccharophilastrainae59te2ishighlyantagonistictowardsmultidrugresistanthumanpathogenswhatdoesitsgenometellus AT desouzayuripinheiroalves aeromonasallosaccharophilastrainae59te2ishighlyantagonistictowardsmultidrugresistanthumanpathogenswhatdoesitsgenometellus AT defreitasalmeidaangelacorrea aeromonasallosaccharophilastrainae59te2ishighlyantagonistictowardsmultidrugresistanthumanpathogenswhatdoesitsgenometellus AT thompsonfabianolopes aeromonasallosaccharophilastrainae59te2ishighlyantagonistictowardsmultidrugresistanthumanpathogenswhatdoesitsgenometellus AT ramosrommelthiagojuca aeromonasallosaccharophilastrainae59te2ishighlyantagonistictowardsmultidrugresistanthumanpathogenswhatdoesitsgenometellus AT whiteleyandrewsteven aeromonasallosaccharophilastrainae59te2ishighlyantagonistictowardsmultidrugresistanthumanpathogenswhatdoesitsgenometellus AT macraeandrew aeromonasallosaccharophilastrainae59te2ishighlyantagonistictowardsmultidrugresistanthumanpathogenswhatdoesitsgenometellus AT deoliveiraselmasoares aeromonasallosaccharophilastrainae59te2ishighlyantagonistictowardsmultidrugresistanthumanpathogenswhatdoesitsgenometellus |