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Talaromyces santanderensis: A New Cadmium-Tolerant Fungus from Cacao Soils in Colombia

Inorganic pollutants in Colombian cocoa (Theobroma cacao L.) agrosystems cause problems in the production, quality, and exportation of this raw material worldwide. There has been an increased interest in bioprospecting studies of different fungal species focused on the biosorption of heavy metals. F...

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Autores principales: Guerra Sierra, Beatriz E., Arteaga-Figueroa, Luis A., Sierra-Pelaéz, Susana, Alvarez, Javier C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9605138/
https://www.ncbi.nlm.nih.gov/pubmed/36294607
http://dx.doi.org/10.3390/jof8101042
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author Guerra Sierra, Beatriz E.
Arteaga-Figueroa, Luis A.
Sierra-Pelaéz, Susana
Alvarez, Javier C.
author_facet Guerra Sierra, Beatriz E.
Arteaga-Figueroa, Luis A.
Sierra-Pelaéz, Susana
Alvarez, Javier C.
author_sort Guerra Sierra, Beatriz E.
collection PubMed
description Inorganic pollutants in Colombian cocoa (Theobroma cacao L.) agrosystems cause problems in the production, quality, and exportation of this raw material worldwide. There has been an increased interest in bioprospecting studies of different fungal species focused on the biosorption of heavy metals. Furthermore, fungi constitute a valuable, profitable, ecological, and efficient natural soil resource that could be considered in the integrated management of cadmium mitigation. This study reports a new species of Talaromyces isolated from a cocoa soil sample collected in San Vicente de Chucurí, Colombia. T. santanderensis is featured by Lemon Yellow (R. Pl. IV) mycelium on CYA, mono-to-biverticillade conidiophores, and acerose phialides. T. santanderensis is distinguished from related species by its growth rate on CYAS and powdery textures on MEA, YES and OA, high acid production on CREA and smaller conidia. It is differentiated from T. lentulus by its growth rate on CYA medium at 37 °C without exudate production, its cream (R. PI. XVI) margin on MEA, and dense sporulation on YES and CYA. Phylogenetic analysis was performed using a polyphasic approach, including different phylogenetic analyses of combined and individual ITS, CaM, BenA, and RPB2 gene sequences that indicate that it is new to science and is named Talaromyces santanderensis sp. nov. This new species belongs to the Talaromyces section and is closely related to T. lentulus, T. soli, T. tumuli, and T. pratensis (inside the T. pinophilus species complex) in the inferred phylogeny. Mycelia growth of the fungal strains was subjected to a range of 0–400 mg/kg Cd and incorporated into malt extract agar (MEA) in triplicates. Fungal radial growth was recorded every three days over a 13-day incubation period and In vitro cadmium tolerance tests showed a high tolerance index (0.81) when the mycelium was exposed to 300 mg/kg of Cd. Results suggest that T. santanderensis showed tolerance to Cd concentrations that exceed the permissible limits for contaminated soils, and it is promising for its use in bioremediation strategies to eliminate Cd from highly contaminated agricultural soils.
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spelling pubmed-96051382022-10-27 Talaromyces santanderensis: A New Cadmium-Tolerant Fungus from Cacao Soils in Colombia Guerra Sierra, Beatriz E. Arteaga-Figueroa, Luis A. Sierra-Pelaéz, Susana Alvarez, Javier C. J Fungi (Basel) Article Inorganic pollutants in Colombian cocoa (Theobroma cacao L.) agrosystems cause problems in the production, quality, and exportation of this raw material worldwide. There has been an increased interest in bioprospecting studies of different fungal species focused on the biosorption of heavy metals. Furthermore, fungi constitute a valuable, profitable, ecological, and efficient natural soil resource that could be considered in the integrated management of cadmium mitigation. This study reports a new species of Talaromyces isolated from a cocoa soil sample collected in San Vicente de Chucurí, Colombia. T. santanderensis is featured by Lemon Yellow (R. Pl. IV) mycelium on CYA, mono-to-biverticillade conidiophores, and acerose phialides. T. santanderensis is distinguished from related species by its growth rate on CYAS and powdery textures on MEA, YES and OA, high acid production on CREA and smaller conidia. It is differentiated from T. lentulus by its growth rate on CYA medium at 37 °C without exudate production, its cream (R. PI. XVI) margin on MEA, and dense sporulation on YES and CYA. Phylogenetic analysis was performed using a polyphasic approach, including different phylogenetic analyses of combined and individual ITS, CaM, BenA, and RPB2 gene sequences that indicate that it is new to science and is named Talaromyces santanderensis sp. nov. This new species belongs to the Talaromyces section and is closely related to T. lentulus, T. soli, T. tumuli, and T. pratensis (inside the T. pinophilus species complex) in the inferred phylogeny. Mycelia growth of the fungal strains was subjected to a range of 0–400 mg/kg Cd and incorporated into malt extract agar (MEA) in triplicates. Fungal radial growth was recorded every three days over a 13-day incubation period and In vitro cadmium tolerance tests showed a high tolerance index (0.81) when the mycelium was exposed to 300 mg/kg of Cd. Results suggest that T. santanderensis showed tolerance to Cd concentrations that exceed the permissible limits for contaminated soils, and it is promising for its use in bioremediation strategies to eliminate Cd from highly contaminated agricultural soils. MDPI 2022-10-01 /pmc/articles/PMC9605138/ /pubmed/36294607 http://dx.doi.org/10.3390/jof8101042 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Guerra Sierra, Beatriz E.
Arteaga-Figueroa, Luis A.
Sierra-Pelaéz, Susana
Alvarez, Javier C.
Talaromyces santanderensis: A New Cadmium-Tolerant Fungus from Cacao Soils in Colombia
title Talaromyces santanderensis: A New Cadmium-Tolerant Fungus from Cacao Soils in Colombia
title_full Talaromyces santanderensis: A New Cadmium-Tolerant Fungus from Cacao Soils in Colombia
title_fullStr Talaromyces santanderensis: A New Cadmium-Tolerant Fungus from Cacao Soils in Colombia
title_full_unstemmed Talaromyces santanderensis: A New Cadmium-Tolerant Fungus from Cacao Soils in Colombia
title_short Talaromyces santanderensis: A New Cadmium-Tolerant Fungus from Cacao Soils in Colombia
title_sort talaromyces santanderensis: a new cadmium-tolerant fungus from cacao soils in colombia
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9605138/
https://www.ncbi.nlm.nih.gov/pubmed/36294607
http://dx.doi.org/10.3390/jof8101042
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