Systemic Resistance Induction of Potato and Tobacco Plants against Potato Virus Y by Klebsiella oxytoca

SIMPLE SUMMARY: Klebsiella oxytoca, as a type of plant growth-promoting rhizobacteria (PGPR), was studied with regards to promoting plant growth and inducing plant systemic resistance against Potato Virus Y (PVY). The results of greenhouse experiments with tobacco and potato plants demonstrated that treatments with the Klebsiella oxytoca and biochar significantly enhanced the growth, while clearly lowering the disease severity and concentration of PVY. An RT-PCR analysis of the defense genes in the tobacco and potato treated with the Klebsiella oxytoca and biochar revealed an association with enhancing the systemic resistance of tobacco and potato to PVY. Klebsiella oxytoca and biochar may be considered valuable options to control PVY in potato and other Solanaceae crops. ABSTRACT: Potato Virus Y (PVY) is a serious potato disease that may significantly decrease potato production. To suppress potato virus infection, several measures have been undertaken. The utilization of plant growth-promoting rhizobacteria is one of these methods. Biochar soil treatment is believed to provide plants with a number of advantages, including increased plant growth and the development of systemic resistance to a variety of plant diseases. The goal of this research was to see whether adding biochar and Klebsiella oxytoca to the soil might cause PVY resistance and enhance the involved mechanisms in PVY resistance. Potato and tobacco seedlings treated with Klebsiella oxytoca and biochar exhibited the same impact of significant symptom reduction, with complete negative ELISA findings, supporting the antiviral activity of K. oxytoca and biochar. Furthermore, owing to the connection between the ISR implicated substrates, significant amounts of polyphenol oxidase, catalase, and superoxide dismutase were observed in treated plants, with the same behavior as defense genes expression levels. It may be a step forward in the development of biochar and K. oxytoca as potential environmentally friendly disease control strategies against PVY.