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Genomic Analysis and Antimicrobial Components of M7, an Aspergillus terreus Strain Derived from the South China Sea

As a typical filamentous fungus, Aspergillus species are highly adaptive to diverse ecological habitats, represented by their occurrence in both terrestrial and marine environments; this could plausibly be ascribed to their preeminent biological diversity and metabolic variability. In this context,...

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Autores principales: Qi, Jiangfeng, Chen, Chaoyi, He, Yajing, Wang, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9605573/
https://www.ncbi.nlm.nih.gov/pubmed/36294615
http://dx.doi.org/10.3390/jof8101051
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author Qi, Jiangfeng
Chen, Chaoyi
He, Yajing
Wang, Ying
author_facet Qi, Jiangfeng
Chen, Chaoyi
He, Yajing
Wang, Ying
author_sort Qi, Jiangfeng
collection PubMed
description As a typical filamentous fungus, Aspergillus species are highly adaptive to diverse ecological habitats, represented by their occurrence in both terrestrial and marine environments; this could plausibly be ascribed to their preeminent biological diversity and metabolic variability. In this context, marine-derived Aspergillus fungi have recently attracted great interest as a promising potential source of biologically active compounds. The present study depicts the genomic and chemical profiles of M7, a strain of Aspergillus terreus isolated from mussels in the South China Sea; the crude extracts of its soybean fermentation exhibit potent growth-inhibitory properties against A. baumannii and P. aeruginosa. Subsequently, functional genomics analysis based on sequences implied a considerable biosynthetic potential of the strain, which is substantiated by the 75 biosynthetic gene clusters (BGCs) identified via genome mining; the majority (49 BGCs) were functionally unknown. Representatively, the putative biosynthetic pathways of terramide A and terramide B, the bacteriostatic products obtained through chemical separation and characterized from the fermentation, could not be allocated to any known BGC, highlighting the metabolic potency and diversity of this strain. Meanwhile, based on a comprehensive analysis of fermentation conditions, we confirmed that the presence of environmental iron was inversely correlated with antimicrobial characteristics of the strain M7, presumably due to the interference in the biosynthetic pathway or bioactive mechanisms of the antimicrobial components, e.g., terramide A and B. Our observations provide genomic and biochemical insight into the metabolic and ecological novelties of this strain, underpinning the diversity of biosynthetic flexibility and adaptive strategies of marine Aspergillus fungi.
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spelling pubmed-96055732022-10-27 Genomic Analysis and Antimicrobial Components of M7, an Aspergillus terreus Strain Derived from the South China Sea Qi, Jiangfeng Chen, Chaoyi He, Yajing Wang, Ying J Fungi (Basel) Article As a typical filamentous fungus, Aspergillus species are highly adaptive to diverse ecological habitats, represented by their occurrence in both terrestrial and marine environments; this could plausibly be ascribed to their preeminent biological diversity and metabolic variability. In this context, marine-derived Aspergillus fungi have recently attracted great interest as a promising potential source of biologically active compounds. The present study depicts the genomic and chemical profiles of M7, a strain of Aspergillus terreus isolated from mussels in the South China Sea; the crude extracts of its soybean fermentation exhibit potent growth-inhibitory properties against A. baumannii and P. aeruginosa. Subsequently, functional genomics analysis based on sequences implied a considerable biosynthetic potential of the strain, which is substantiated by the 75 biosynthetic gene clusters (BGCs) identified via genome mining; the majority (49 BGCs) were functionally unknown. Representatively, the putative biosynthetic pathways of terramide A and terramide B, the bacteriostatic products obtained through chemical separation and characterized from the fermentation, could not be allocated to any known BGC, highlighting the metabolic potency and diversity of this strain. Meanwhile, based on a comprehensive analysis of fermentation conditions, we confirmed that the presence of environmental iron was inversely correlated with antimicrobial characteristics of the strain M7, presumably due to the interference in the biosynthetic pathway or bioactive mechanisms of the antimicrobial components, e.g., terramide A and B. Our observations provide genomic and biochemical insight into the metabolic and ecological novelties of this strain, underpinning the diversity of biosynthetic flexibility and adaptive strategies of marine Aspergillus fungi. MDPI 2022-10-07 /pmc/articles/PMC9605573/ /pubmed/36294615 http://dx.doi.org/10.3390/jof8101051 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Qi, Jiangfeng
Chen, Chaoyi
He, Yajing
Wang, Ying
Genomic Analysis and Antimicrobial Components of M7, an Aspergillus terreus Strain Derived from the South China Sea
title Genomic Analysis and Antimicrobial Components of M7, an Aspergillus terreus Strain Derived from the South China Sea
title_full Genomic Analysis and Antimicrobial Components of M7, an Aspergillus terreus Strain Derived from the South China Sea
title_fullStr Genomic Analysis and Antimicrobial Components of M7, an Aspergillus terreus Strain Derived from the South China Sea
title_full_unstemmed Genomic Analysis and Antimicrobial Components of M7, an Aspergillus terreus Strain Derived from the South China Sea
title_short Genomic Analysis and Antimicrobial Components of M7, an Aspergillus terreus Strain Derived from the South China Sea
title_sort genomic analysis and antimicrobial components of m7, an aspergillus terreus strain derived from the south china sea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9605573/
https://www.ncbi.nlm.nih.gov/pubmed/36294615
http://dx.doi.org/10.3390/jof8101051
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