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Validation of the 3M(TM) Molecular Detection Assay 2—Salmonella for detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: AOAC Performance Tested Methods(SM) 091501

BACKGROUND: The 3M™ Molecular Detection Assay 2—Salmonella method is based on real-time loop-mediated isothermal amplification when used with the 3M Molecular Detection System for the rapid and specific detection of Salmonella in enriched products. The 3M Molecular Detection Assay 2—Salmonella was a...

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Autores principales: Rosauer, Micki L, Lopez-Velasco, Gabriela, Silbernagel, Karen M, Horine, Leo, Tudor, Alexandra
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9605774/
https://www.ncbi.nlm.nih.gov/pubmed/35737412
http://dx.doi.org/10.1093/jaoacint/qsac068
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author Rosauer, Micki L
Lopez-Velasco, Gabriela
Silbernagel, Karen M
Horine, Leo
Tudor, Alexandra
author_facet Rosauer, Micki L
Lopez-Velasco, Gabriela
Silbernagel, Karen M
Horine, Leo
Tudor, Alexandra
author_sort Rosauer, Micki L
collection PubMed
description BACKGROUND: The 3M™ Molecular Detection Assay 2—Salmonella method is based on real-time loop-mediated isothermal amplification when used with the 3M Molecular Detection System for the rapid and specific detection of Salmonella in enriched products. The 3M Molecular Detection Assay 2—Salmonella was approved as AOAC INTERNATIONAL (AOAC) Performance Tested Method(SM) (PTM) Certificate No. 091501 and as AOAC Official Method of Analysis(SM)2016.01. OBJECTIVE: This matrix extension study evaluated the 3M Molecular Detection Assay 2—Salmonella for detection of Salmonella in dried cannabis flower [>0.3% delta 9-tetrahydrocannabinol (THC)] and dried hemp flower (≤0.3% THC) at a 10 g test portion size. METHOD: Matrix studies in dried cannabis and hemp flowers followed procedures outlined in 3M Molecular Detection Assay 2—Salmonella product instructions and Standard Method Performance Requirement (SMPR(®)) for Detection of Salmonella species in Cannabis and Cannabis Products (AOAC SMPR 2020.002). The method was evaluated at low, high, and non-contaminated levels. RESULTS: Results showed no statistically significant difference between the presumptive positive 3M Molecular Detection Assay 2—Salmonella results and the SMPR 2020.002 recommended cultural confirmations. CONCLUSIONS: This study demonstrates that the 3M Molecular Detection Assay 2—Salmonella is a reliable method for the rapid and specific detection of Salmonella in dried cannabis flower and dried hemp flower. HIGHLIGHTS: The 3M Molecular Detection Assay 2—Salmonella method is suitable for the rapid and specific detection of Salmonella in dried cannabis flower and dried hemp flower.
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spelling pubmed-96057742022-10-31 Validation of the 3M(TM) Molecular Detection Assay 2—Salmonella for detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: AOAC Performance Tested Methods(SM) 091501 Rosauer, Micki L Lopez-Velasco, Gabriela Silbernagel, Karen M Horine, Leo Tudor, Alexandra J AOAC Int Short Communication BACKGROUND: The 3M™ Molecular Detection Assay 2—Salmonella method is based on real-time loop-mediated isothermal amplification when used with the 3M Molecular Detection System for the rapid and specific detection of Salmonella in enriched products. The 3M Molecular Detection Assay 2—Salmonella was approved as AOAC INTERNATIONAL (AOAC) Performance Tested Method(SM) (PTM) Certificate No. 091501 and as AOAC Official Method of Analysis(SM)2016.01. OBJECTIVE: This matrix extension study evaluated the 3M Molecular Detection Assay 2—Salmonella for detection of Salmonella in dried cannabis flower [>0.3% delta 9-tetrahydrocannabinol (THC)] and dried hemp flower (≤0.3% THC) at a 10 g test portion size. METHOD: Matrix studies in dried cannabis and hemp flowers followed procedures outlined in 3M Molecular Detection Assay 2—Salmonella product instructions and Standard Method Performance Requirement (SMPR(®)) for Detection of Salmonella species in Cannabis and Cannabis Products (AOAC SMPR 2020.002). The method was evaluated at low, high, and non-contaminated levels. RESULTS: Results showed no statistically significant difference between the presumptive positive 3M Molecular Detection Assay 2—Salmonella results and the SMPR 2020.002 recommended cultural confirmations. CONCLUSIONS: This study demonstrates that the 3M Molecular Detection Assay 2—Salmonella is a reliable method for the rapid and specific detection of Salmonella in dried cannabis flower and dried hemp flower. HIGHLIGHTS: The 3M Molecular Detection Assay 2—Salmonella method is suitable for the rapid and specific detection of Salmonella in dried cannabis flower and dried hemp flower. Oxford University Press 2022-06-23 /pmc/articles/PMC9605774/ /pubmed/35737412 http://dx.doi.org/10.1093/jaoacint/qsac068 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of AOAC INTERNATIONAL. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Short Communication
Rosauer, Micki L
Lopez-Velasco, Gabriela
Silbernagel, Karen M
Horine, Leo
Tudor, Alexandra
Validation of the 3M(TM) Molecular Detection Assay 2—Salmonella for detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: AOAC Performance Tested Methods(SM) 091501
title Validation of the 3M(TM) Molecular Detection Assay 2—Salmonella for detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: AOAC Performance Tested Methods(SM) 091501
title_full Validation of the 3M(TM) Molecular Detection Assay 2—Salmonella for detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: AOAC Performance Tested Methods(SM) 091501
title_fullStr Validation of the 3M(TM) Molecular Detection Assay 2—Salmonella for detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: AOAC Performance Tested Methods(SM) 091501
title_full_unstemmed Validation of the 3M(TM) Molecular Detection Assay 2—Salmonella for detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: AOAC Performance Tested Methods(SM) 091501
title_short Validation of the 3M(TM) Molecular Detection Assay 2—Salmonella for detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: AOAC Performance Tested Methods(SM) 091501
title_sort validation of the 3m(tm) molecular detection assay 2—salmonella for detection of salmonella in dried cannabis flower and dried hemp flower: aoac performance tested methods(sm) 091501
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9605774/
https://www.ncbi.nlm.nih.gov/pubmed/35737412
http://dx.doi.org/10.1093/jaoacint/qsac068
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