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Validation of the 3M(TM) Molecular Detection Assay 2—Salmonella for detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: AOAC Performance Tested Methods(SM) 091501
BACKGROUND: The 3M™ Molecular Detection Assay 2—Salmonella method is based on real-time loop-mediated isothermal amplification when used with the 3M Molecular Detection System for the rapid and specific detection of Salmonella in enriched products. The 3M Molecular Detection Assay 2—Salmonella was a...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9605774/ https://www.ncbi.nlm.nih.gov/pubmed/35737412 http://dx.doi.org/10.1093/jaoacint/qsac068 |
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author | Rosauer, Micki L Lopez-Velasco, Gabriela Silbernagel, Karen M Horine, Leo Tudor, Alexandra |
author_facet | Rosauer, Micki L Lopez-Velasco, Gabriela Silbernagel, Karen M Horine, Leo Tudor, Alexandra |
author_sort | Rosauer, Micki L |
collection | PubMed |
description | BACKGROUND: The 3M™ Molecular Detection Assay 2—Salmonella method is based on real-time loop-mediated isothermal amplification when used with the 3M Molecular Detection System for the rapid and specific detection of Salmonella in enriched products. The 3M Molecular Detection Assay 2—Salmonella was approved as AOAC INTERNATIONAL (AOAC) Performance Tested Method(SM) (PTM) Certificate No. 091501 and as AOAC Official Method of Analysis(SM)2016.01. OBJECTIVE: This matrix extension study evaluated the 3M Molecular Detection Assay 2—Salmonella for detection of Salmonella in dried cannabis flower [>0.3% delta 9-tetrahydrocannabinol (THC)] and dried hemp flower (≤0.3% THC) at a 10 g test portion size. METHOD: Matrix studies in dried cannabis and hemp flowers followed procedures outlined in 3M Molecular Detection Assay 2—Salmonella product instructions and Standard Method Performance Requirement (SMPR(®)) for Detection of Salmonella species in Cannabis and Cannabis Products (AOAC SMPR 2020.002). The method was evaluated at low, high, and non-contaminated levels. RESULTS: Results showed no statistically significant difference between the presumptive positive 3M Molecular Detection Assay 2—Salmonella results and the SMPR 2020.002 recommended cultural confirmations. CONCLUSIONS: This study demonstrates that the 3M Molecular Detection Assay 2—Salmonella is a reliable method for the rapid and specific detection of Salmonella in dried cannabis flower and dried hemp flower. HIGHLIGHTS: The 3M Molecular Detection Assay 2—Salmonella method is suitable for the rapid and specific detection of Salmonella in dried cannabis flower and dried hemp flower. |
format | Online Article Text |
id | pubmed-9605774 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-96057742022-10-31 Validation of the 3M(TM) Molecular Detection Assay 2—Salmonella for detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: AOAC Performance Tested Methods(SM) 091501 Rosauer, Micki L Lopez-Velasco, Gabriela Silbernagel, Karen M Horine, Leo Tudor, Alexandra J AOAC Int Short Communication BACKGROUND: The 3M™ Molecular Detection Assay 2—Salmonella method is based on real-time loop-mediated isothermal amplification when used with the 3M Molecular Detection System for the rapid and specific detection of Salmonella in enriched products. The 3M Molecular Detection Assay 2—Salmonella was approved as AOAC INTERNATIONAL (AOAC) Performance Tested Method(SM) (PTM) Certificate No. 091501 and as AOAC Official Method of Analysis(SM)2016.01. OBJECTIVE: This matrix extension study evaluated the 3M Molecular Detection Assay 2—Salmonella for detection of Salmonella in dried cannabis flower [>0.3% delta 9-tetrahydrocannabinol (THC)] and dried hemp flower (≤0.3% THC) at a 10 g test portion size. METHOD: Matrix studies in dried cannabis and hemp flowers followed procedures outlined in 3M Molecular Detection Assay 2—Salmonella product instructions and Standard Method Performance Requirement (SMPR(®)) for Detection of Salmonella species in Cannabis and Cannabis Products (AOAC SMPR 2020.002). The method was evaluated at low, high, and non-contaminated levels. RESULTS: Results showed no statistically significant difference between the presumptive positive 3M Molecular Detection Assay 2—Salmonella results and the SMPR 2020.002 recommended cultural confirmations. CONCLUSIONS: This study demonstrates that the 3M Molecular Detection Assay 2—Salmonella is a reliable method for the rapid and specific detection of Salmonella in dried cannabis flower and dried hemp flower. HIGHLIGHTS: The 3M Molecular Detection Assay 2—Salmonella method is suitable for the rapid and specific detection of Salmonella in dried cannabis flower and dried hemp flower. Oxford University Press 2022-06-23 /pmc/articles/PMC9605774/ /pubmed/35737412 http://dx.doi.org/10.1093/jaoacint/qsac068 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of AOAC INTERNATIONAL. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com |
spellingShingle | Short Communication Rosauer, Micki L Lopez-Velasco, Gabriela Silbernagel, Karen M Horine, Leo Tudor, Alexandra Validation of the 3M(TM) Molecular Detection Assay 2—Salmonella for detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: AOAC Performance Tested Methods(SM) 091501 |
title | Validation of the 3M(TM) Molecular Detection Assay 2—Salmonella for detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: AOAC Performance Tested Methods(SM) 091501 |
title_full | Validation of the 3M(TM) Molecular Detection Assay 2—Salmonella for detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: AOAC Performance Tested Methods(SM) 091501 |
title_fullStr | Validation of the 3M(TM) Molecular Detection Assay 2—Salmonella for detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: AOAC Performance Tested Methods(SM) 091501 |
title_full_unstemmed | Validation of the 3M(TM) Molecular Detection Assay 2—Salmonella for detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: AOAC Performance Tested Methods(SM) 091501 |
title_short | Validation of the 3M(TM) Molecular Detection Assay 2—Salmonella for detection of Salmonella in Dried Cannabis Flower and Dried Hemp Flower: AOAC Performance Tested Methods(SM) 091501 |
title_sort | validation of the 3m(tm) molecular detection assay 2—salmonella for detection of salmonella in dried cannabis flower and dried hemp flower: aoac performance tested methods(sm) 091501 |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9605774/ https://www.ncbi.nlm.nih.gov/pubmed/35737412 http://dx.doi.org/10.1093/jaoacint/qsac068 |
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