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Histone methyltransferases SDG33 and SDG34 regulate organ-specific nitrogen responses in tomato

Histone posttranslational modifications shape the chromatin landscape of the plant genome and affect gene expression in response to developmental and environmental cues. To date, the role of histone modifications in regulating plant responses to environmental nutrient availability, especially in agr...

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Autores principales: Bvindi, Carol, Tang, Liang, Lee, Sanghun, Patrick, Ryan M., Yee, Zheng Rong, Mengiste, Tesfaye, Li, Ying
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9606235/
https://www.ncbi.nlm.nih.gov/pubmed/36311072
http://dx.doi.org/10.3389/fpls.2022.1005077
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author Bvindi, Carol
Tang, Liang
Lee, Sanghun
Patrick, Ryan M.
Yee, Zheng Rong
Mengiste, Tesfaye
Li, Ying
author_facet Bvindi, Carol
Tang, Liang
Lee, Sanghun
Patrick, Ryan M.
Yee, Zheng Rong
Mengiste, Tesfaye
Li, Ying
author_sort Bvindi, Carol
collection PubMed
description Histone posttranslational modifications shape the chromatin landscape of the plant genome and affect gene expression in response to developmental and environmental cues. To date, the role of histone modifications in regulating plant responses to environmental nutrient availability, especially in agriculturally important species, remains largely unknown. We describe the functions of two histone lysine methyltransferases, SET Domain Group 33 (SDG33) and SDG34, in mediating nitrogen (N) responses of shoots and roots in tomato. By comparing the transcriptomes of CRISPR edited tomato lines sdg33 and sdg34 with wild-type plants under N-supplied and N-starved conditions, we uncovered that SDG33 and SDG34 regulate overlapping yet distinct downstream gene targets. In response to N level changes, both SDG33 and SDG34 mediate gene regulation in an organ-specific manner: in roots, SDG33 and SDG34 regulate a gene network including Nitrate Transporter 1.1 (NRT1.1) and Small Auxin Up-regulated RNA (SAUR) genes. In agreement with this, mutations in sdg33 or sdg34 abolish the root growth response triggered by an N-supply; In shoots, SDG33 and SDG34 affect the expression of photosynthesis genes and photosynthetic parameters in response to N. Our analysis thus revealed that SDG33 and SDG34 regulate N-responsive gene expression and physiological changes in an organ-specific manner, thus presenting previously unknown candidate genes as targets for selection and engineering to improve N uptake and usage in crop plants.
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spelling pubmed-96062352022-10-28 Histone methyltransferases SDG33 and SDG34 regulate organ-specific nitrogen responses in tomato Bvindi, Carol Tang, Liang Lee, Sanghun Patrick, Ryan M. Yee, Zheng Rong Mengiste, Tesfaye Li, Ying Front Plant Sci Plant Science Histone posttranslational modifications shape the chromatin landscape of the plant genome and affect gene expression in response to developmental and environmental cues. To date, the role of histone modifications in regulating plant responses to environmental nutrient availability, especially in agriculturally important species, remains largely unknown. We describe the functions of two histone lysine methyltransferases, SET Domain Group 33 (SDG33) and SDG34, in mediating nitrogen (N) responses of shoots and roots in tomato. By comparing the transcriptomes of CRISPR edited tomato lines sdg33 and sdg34 with wild-type plants under N-supplied and N-starved conditions, we uncovered that SDG33 and SDG34 regulate overlapping yet distinct downstream gene targets. In response to N level changes, both SDG33 and SDG34 mediate gene regulation in an organ-specific manner: in roots, SDG33 and SDG34 regulate a gene network including Nitrate Transporter 1.1 (NRT1.1) and Small Auxin Up-regulated RNA (SAUR) genes. In agreement with this, mutations in sdg33 or sdg34 abolish the root growth response triggered by an N-supply; In shoots, SDG33 and SDG34 affect the expression of photosynthesis genes and photosynthetic parameters in response to N. Our analysis thus revealed that SDG33 and SDG34 regulate N-responsive gene expression and physiological changes in an organ-specific manner, thus presenting previously unknown candidate genes as targets for selection and engineering to improve N uptake and usage in crop plants. Frontiers Media S.A. 2022-10-12 /pmc/articles/PMC9606235/ /pubmed/36311072 http://dx.doi.org/10.3389/fpls.2022.1005077 Text en Copyright © 2022 Bvindi, Tang, Lee, Patrick, Yee, Mengiste and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Bvindi, Carol
Tang, Liang
Lee, Sanghun
Patrick, Ryan M.
Yee, Zheng Rong
Mengiste, Tesfaye
Li, Ying
Histone methyltransferases SDG33 and SDG34 regulate organ-specific nitrogen responses in tomato
title Histone methyltransferases SDG33 and SDG34 regulate organ-specific nitrogen responses in tomato
title_full Histone methyltransferases SDG33 and SDG34 regulate organ-specific nitrogen responses in tomato
title_fullStr Histone methyltransferases SDG33 and SDG34 regulate organ-specific nitrogen responses in tomato
title_full_unstemmed Histone methyltransferases SDG33 and SDG34 regulate organ-specific nitrogen responses in tomato
title_short Histone methyltransferases SDG33 and SDG34 regulate organ-specific nitrogen responses in tomato
title_sort histone methyltransferases sdg33 and sdg34 regulate organ-specific nitrogen responses in tomato
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9606235/
https://www.ncbi.nlm.nih.gov/pubmed/36311072
http://dx.doi.org/10.3389/fpls.2022.1005077
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