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Enhancing stability of recombinant CHO cells by CRISPR/Cas9-mediated site-specific integration into regions with distinct histone modifications

Chinese hamster ovary (CHO) cells are the most important platform for producing biotherapeutics. Random integration of a transgene into epigenetically instable regions of the genome results in silencing of the gene of interest and loss of productivity during upstream processing. Therefore, cost- and...

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Detalles Bibliográficos
Autores principales:	Hertel, Oliver, Neuss, Anne, Busche, Tobias, Brandt, David, Kalinowski, Jörn, Bahnemann, Janina, Noll, Thomas
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2022
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9606416/ https://www.ncbi.nlm.nih.gov/pubmed/36312557 http://dx.doi.org/10.3389/fbioe.2022.1010719

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