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IL-10 regulates Th17 response to inhibit hepatobiliary injury caused by Clonorchis sinensis infection in C57BL/6J mice

Clonorchiasis caused by Clonorchis sinensis is a mainly foodborne parasitic disease. It can lead to hepatobiliary duct inflammation, fibrosis, obstructive jaundice, liver cirrhosis, and even cholangiocarcinoma. Interleukin (IL)-10 is an immune-regulatory cytokine which plays an immunosuppressive rol...

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Autores principales: Zhang, Beibei, Wang, Jianling, Liu, Man, Zhao, Qianqian, Yu, Guozhi, Zhang, Bo, Hua, Hui, Xu, Jinyao, Li, Jing, Yu, Qian, Koda, Stephane, Xu, Yin-Hai, Jiang, Zhihua, Yan, Chao, Zheng, Kui-Yang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9606589/
https://www.ncbi.nlm.nih.gov/pubmed/36310865
http://dx.doi.org/10.3389/fcimb.2022.994838
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author Zhang, Beibei
Wang, Jianling
Liu, Man
Zhao, Qianqian
Yu, Guozhi
Zhang, Bo
Hua, Hui
Xu, Jinyao
Li, Jing
Yu, Qian
Koda, Stephane
Xu, Yin-Hai
Jiang, Zhihua
Yan, Chao
Zheng, Kui-Yang
author_facet Zhang, Beibei
Wang, Jianling
Liu, Man
Zhao, Qianqian
Yu, Guozhi
Zhang, Bo
Hua, Hui
Xu, Jinyao
Li, Jing
Yu, Qian
Koda, Stephane
Xu, Yin-Hai
Jiang, Zhihua
Yan, Chao
Zheng, Kui-Yang
author_sort Zhang, Beibei
collection PubMed
description Clonorchiasis caused by Clonorchis sinensis is a mainly foodborne parasitic disease. It can lead to hepatobiliary duct inflammation, fibrosis, obstructive jaundice, liver cirrhosis, and even cholangiocarcinoma. Interleukin (IL)-10 is an immune-regulatory cytokine which plays an immunosuppressive role during infection. Our previous study found that IL-10 was increased in mice with C. sinensis infection. However, the role and mechanism of IL-10 playing in hepatobiliary injury induced by C. sinensis infection remain unknown. Herein, Il10(+/+) mice and Il10(+/-) C57BL/6J mice were infected with C. sinensis. It was found that IL-10 deficiency aggravated biliary hyperplasia and exacerbated periductal fibrosis induced by C. sinensis infection. Moreover, IL-10 deficiency increased CD4(+)T cells and CD8(+)T cells but not macrophages in the liver of mice with infection. There were no apparent differences in Th1 and Treg cells between Il10(+/+) and Il10(+/-) mice infected with C. sinensis. However, the proportion of Th17 cells in CD4(+)T cells in Il10(+/-) infected mice was significantly higher than that in Il10(+/+) infected mice. IL-10 deficiency also enhanced the increase of Th17 cells induced by ESPs stimulation in vitro. Taken together, our results suggest that IL-10 plays a protective role in hepatobiliary injury in C57BL/6J mice induced by C. sinensis infection via inhibiting Th17 cells, which could deepen our understanding of the immunopathology of clonorchiasis.
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spelling pubmed-96065892022-10-28 IL-10 regulates Th17 response to inhibit hepatobiliary injury caused by Clonorchis sinensis infection in C57BL/6J mice Zhang, Beibei Wang, Jianling Liu, Man Zhao, Qianqian Yu, Guozhi Zhang, Bo Hua, Hui Xu, Jinyao Li, Jing Yu, Qian Koda, Stephane Xu, Yin-Hai Jiang, Zhihua Yan, Chao Zheng, Kui-Yang Front Cell Infect Microbiol Cellular and Infection Microbiology Clonorchiasis caused by Clonorchis sinensis is a mainly foodborne parasitic disease. It can lead to hepatobiliary duct inflammation, fibrosis, obstructive jaundice, liver cirrhosis, and even cholangiocarcinoma. Interleukin (IL)-10 is an immune-regulatory cytokine which plays an immunosuppressive role during infection. Our previous study found that IL-10 was increased in mice with C. sinensis infection. However, the role and mechanism of IL-10 playing in hepatobiliary injury induced by C. sinensis infection remain unknown. Herein, Il10(+/+) mice and Il10(+/-) C57BL/6J mice were infected with C. sinensis. It was found that IL-10 deficiency aggravated biliary hyperplasia and exacerbated periductal fibrosis induced by C. sinensis infection. Moreover, IL-10 deficiency increased CD4(+)T cells and CD8(+)T cells but not macrophages in the liver of mice with infection. There were no apparent differences in Th1 and Treg cells between Il10(+/+) and Il10(+/-) mice infected with C. sinensis. However, the proportion of Th17 cells in CD4(+)T cells in Il10(+/-) infected mice was significantly higher than that in Il10(+/+) infected mice. IL-10 deficiency also enhanced the increase of Th17 cells induced by ESPs stimulation in vitro. Taken together, our results suggest that IL-10 plays a protective role in hepatobiliary injury in C57BL/6J mice induced by C. sinensis infection via inhibiting Th17 cells, which could deepen our understanding of the immunopathology of clonorchiasis. Frontiers Media S.A. 2022-10-13 /pmc/articles/PMC9606589/ /pubmed/36310865 http://dx.doi.org/10.3389/fcimb.2022.994838 Text en Copyright © 2022 Zhang, Wang, Liu, Zhao, Yu, Zhang, Hua, Xu, Li, Yu, Koda, Xu, Jiang, Yan and Zheng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Zhang, Beibei
Wang, Jianling
Liu, Man
Zhao, Qianqian
Yu, Guozhi
Zhang, Bo
Hua, Hui
Xu, Jinyao
Li, Jing
Yu, Qian
Koda, Stephane
Xu, Yin-Hai
Jiang, Zhihua
Yan, Chao
Zheng, Kui-Yang
IL-10 regulates Th17 response to inhibit hepatobiliary injury caused by Clonorchis sinensis infection in C57BL/6J mice
title IL-10 regulates Th17 response to inhibit hepatobiliary injury caused by Clonorchis sinensis infection in C57BL/6J mice
title_full IL-10 regulates Th17 response to inhibit hepatobiliary injury caused by Clonorchis sinensis infection in C57BL/6J mice
title_fullStr IL-10 regulates Th17 response to inhibit hepatobiliary injury caused by Clonorchis sinensis infection in C57BL/6J mice
title_full_unstemmed IL-10 regulates Th17 response to inhibit hepatobiliary injury caused by Clonorchis sinensis infection in C57BL/6J mice
title_short IL-10 regulates Th17 response to inhibit hepatobiliary injury caused by Clonorchis sinensis infection in C57BL/6J mice
title_sort il-10 regulates th17 response to inhibit hepatobiliary injury caused by clonorchis sinensis infection in c57bl/6j mice
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9606589/
https://www.ncbi.nlm.nih.gov/pubmed/36310865
http://dx.doi.org/10.3389/fcimb.2022.994838
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