Cargando…

Genetically engineered bacterium: Principles, practices, and prospects

Advances in synthetic biology and the clinical application of bacteriotherapy enable the use of genetically engineered bacteria (GEB) to combat various diseases. GEB act as a small ‘machine factory’ in the intestine or other tissues to continuously produce heterologous proteins or molecular compound...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Yiting, Feng, Jing, Pan, Hangcheng, Zhang, Xiuwei, Zhang, Yunlei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9606703/
https://www.ncbi.nlm.nih.gov/pubmed/36312915
http://dx.doi.org/10.3389/fmicb.2022.997587
_version_ 1784818356007731200
author Liu, Yiting
Feng, Jing
Pan, Hangcheng
Zhang, Xiuwei
Zhang, Yunlei
author_facet Liu, Yiting
Feng, Jing
Pan, Hangcheng
Zhang, Xiuwei
Zhang, Yunlei
author_sort Liu, Yiting
collection PubMed
description Advances in synthetic biology and the clinical application of bacteriotherapy enable the use of genetically engineered bacteria (GEB) to combat various diseases. GEB act as a small ‘machine factory’ in the intestine or other tissues to continuously produce heterologous proteins or molecular compounds and, thus, diagnose or cure disease or work as an adjuvant reagent for disease treatment by regulating the immune system. Although the achievements of GEBs in the treatment or adjuvant therapy of diseases are promising, the practical implementation of this new therapeutic modality remains a grand challenge, especially at the initial stage. In this review, we introduce the development of GEBs and their advantages in disease management, summarize the latest research advances in microbial genetic techniques, and discuss their administration routes, performance indicators and the limitations of GEBs used as platforms for disease management. We also present several examples of GEB applications in the treatment of cancers and metabolic diseases and further highlight their great potential for clinical application in the near future.
format Online
Article
Text
id pubmed-9606703
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-96067032022-10-28 Genetically engineered bacterium: Principles, practices, and prospects Liu, Yiting Feng, Jing Pan, Hangcheng Zhang, Xiuwei Zhang, Yunlei Front Microbiol Microbiology Advances in synthetic biology and the clinical application of bacteriotherapy enable the use of genetically engineered bacteria (GEB) to combat various diseases. GEB act as a small ‘machine factory’ in the intestine or other tissues to continuously produce heterologous proteins or molecular compounds and, thus, diagnose or cure disease or work as an adjuvant reagent for disease treatment by regulating the immune system. Although the achievements of GEBs in the treatment or adjuvant therapy of diseases are promising, the practical implementation of this new therapeutic modality remains a grand challenge, especially at the initial stage. In this review, we introduce the development of GEBs and their advantages in disease management, summarize the latest research advances in microbial genetic techniques, and discuss their administration routes, performance indicators and the limitations of GEBs used as platforms for disease management. We also present several examples of GEB applications in the treatment of cancers and metabolic diseases and further highlight their great potential for clinical application in the near future. Frontiers Media S.A. 2022-10-13 /pmc/articles/PMC9606703/ /pubmed/36312915 http://dx.doi.org/10.3389/fmicb.2022.997587 Text en Copyright © 2022 Liu, Feng, Pan, Zhang and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Liu, Yiting
Feng, Jing
Pan, Hangcheng
Zhang, Xiuwei
Zhang, Yunlei
Genetically engineered bacterium: Principles, practices, and prospects
title Genetically engineered bacterium: Principles, practices, and prospects
title_full Genetically engineered bacterium: Principles, practices, and prospects
title_fullStr Genetically engineered bacterium: Principles, practices, and prospects
title_full_unstemmed Genetically engineered bacterium: Principles, practices, and prospects
title_short Genetically engineered bacterium: Principles, practices, and prospects
title_sort genetically engineered bacterium: principles, practices, and prospects
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9606703/
https://www.ncbi.nlm.nih.gov/pubmed/36312915
http://dx.doi.org/10.3389/fmicb.2022.997587
work_keys_str_mv AT liuyiting geneticallyengineeredbacteriumprinciplespracticesandprospects
AT fengjing geneticallyengineeredbacteriumprinciplespracticesandprospects
AT panhangcheng geneticallyengineeredbacteriumprinciplespracticesandprospects
AT zhangxiuwei geneticallyengineeredbacteriumprinciplespracticesandprospects
AT zhangyunlei geneticallyengineeredbacteriumprinciplespracticesandprospects