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Genetic characterization of porcine reproductive and respiratory syndrome virus from Eastern China during 2017–2022

Porcine reproductive and respiratory syndrome (PRRS) is an immunosuppressive disease caused by PRSS virus (PRRSV). PRRSV mainly causes reproductive disorders in pregnant sows and respiratory diseases in piglets. Recently, it has emerged as one of the most important diseases of the pig industry acros...

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Autores principales: Zhou, Lujia, Yang, Yang, Xia, Qiqi, Guan, Zhixin, Zhang, Junjie, Li, Beibei, Qiu, Yafeng, Liu, Ke, Shao, Donghua, Ma, Zhiyong, Wang, Xiaodu, Wei, Jianchao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9606797/
https://www.ncbi.nlm.nih.gov/pubmed/36312912
http://dx.doi.org/10.3389/fmicb.2022.971817
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author Zhou, Lujia
Yang, Yang
Xia, Qiqi
Guan, Zhixin
Zhang, Junjie
Li, Beibei
Qiu, Yafeng
Liu, Ke
Shao, Donghua
Ma, Zhiyong
Wang, Xiaodu
Wei, Jianchao
author_facet Zhou, Lujia
Yang, Yang
Xia, Qiqi
Guan, Zhixin
Zhang, Junjie
Li, Beibei
Qiu, Yafeng
Liu, Ke
Shao, Donghua
Ma, Zhiyong
Wang, Xiaodu
Wei, Jianchao
author_sort Zhou, Lujia
collection PubMed
description Porcine reproductive and respiratory syndrome (PRRS) is an immunosuppressive disease caused by PRSS virus (PRRSV). PRRSV mainly causes reproductive disorders in pregnant sows and respiratory diseases in piglets. Recently, it has emerged as one of the most important diseases of the pig industry across the globe. In this study, we have collected 231 samples from differently sized pig farms in Eastern China from 2017 to 2022 to investigate the epidemic characteristics of the disease. All samples were screened by RT-PCR and analyzed further using Nsp2 and ORF5 genes. The result showed that the positive rate of PRRSV was 24% (54/231). Phylogenetic analysis (13 positive samples) revealed that all isolates belonged to genotype 2, and they were mainly distributed in four lineages (i.e., lineage 1, 3, 5, and 8). Nsp2 is the most variable protein among all PRRSV NSPs, several isolates from this study had amino acid deletions within Nsp2 compared to that of strain VR-2332. The major structural protein glycoprotein (GP5) protein is encoded by ORF5. Epitope analysis of the 13 isolated strains and additional reference strains revealed that all 13 strains had some mutations on the decoy epitope, the primary neutralizing epitope, T cell epitopes, and B cell epitopes. This study showed that the prevalent PRRSV strain in Eastern China was still HP-PRRSV, while the proportion of NADC30-like and NADC34-like strains have increased. This study further enriches the epidemiological data of PRRS in Eastern China and provides a theoretical basis for vaccine development and prevention and control of the disease across the region.
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spelling pubmed-96067972022-10-28 Genetic characterization of porcine reproductive and respiratory syndrome virus from Eastern China during 2017–2022 Zhou, Lujia Yang, Yang Xia, Qiqi Guan, Zhixin Zhang, Junjie Li, Beibei Qiu, Yafeng Liu, Ke Shao, Donghua Ma, Zhiyong Wang, Xiaodu Wei, Jianchao Front Microbiol Microbiology Porcine reproductive and respiratory syndrome (PRRS) is an immunosuppressive disease caused by PRSS virus (PRRSV). PRRSV mainly causes reproductive disorders in pregnant sows and respiratory diseases in piglets. Recently, it has emerged as one of the most important diseases of the pig industry across the globe. In this study, we have collected 231 samples from differently sized pig farms in Eastern China from 2017 to 2022 to investigate the epidemic characteristics of the disease. All samples were screened by RT-PCR and analyzed further using Nsp2 and ORF5 genes. The result showed that the positive rate of PRRSV was 24% (54/231). Phylogenetic analysis (13 positive samples) revealed that all isolates belonged to genotype 2, and they were mainly distributed in four lineages (i.e., lineage 1, 3, 5, and 8). Nsp2 is the most variable protein among all PRRSV NSPs, several isolates from this study had amino acid deletions within Nsp2 compared to that of strain VR-2332. The major structural protein glycoprotein (GP5) protein is encoded by ORF5. Epitope analysis of the 13 isolated strains and additional reference strains revealed that all 13 strains had some mutations on the decoy epitope, the primary neutralizing epitope, T cell epitopes, and B cell epitopes. This study showed that the prevalent PRRSV strain in Eastern China was still HP-PRRSV, while the proportion of NADC30-like and NADC34-like strains have increased. This study further enriches the epidemiological data of PRRS in Eastern China and provides a theoretical basis for vaccine development and prevention and control of the disease across the region. Frontiers Media S.A. 2022-10-13 /pmc/articles/PMC9606797/ /pubmed/36312912 http://dx.doi.org/10.3389/fmicb.2022.971817 Text en Copyright © 2022 Zhou, Yang, Xia, Guan, Zhang, Li, Qiu, Liu, Shao, Ma, Wang and Wei. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Zhou, Lujia
Yang, Yang
Xia, Qiqi
Guan, Zhixin
Zhang, Junjie
Li, Beibei
Qiu, Yafeng
Liu, Ke
Shao, Donghua
Ma, Zhiyong
Wang, Xiaodu
Wei, Jianchao
Genetic characterization of porcine reproductive and respiratory syndrome virus from Eastern China during 2017–2022
title Genetic characterization of porcine reproductive and respiratory syndrome virus from Eastern China during 2017–2022
title_full Genetic characterization of porcine reproductive and respiratory syndrome virus from Eastern China during 2017–2022
title_fullStr Genetic characterization of porcine reproductive and respiratory syndrome virus from Eastern China during 2017–2022
title_full_unstemmed Genetic characterization of porcine reproductive and respiratory syndrome virus from Eastern China during 2017–2022
title_short Genetic characterization of porcine reproductive and respiratory syndrome virus from Eastern China during 2017–2022
title_sort genetic characterization of porcine reproductive and respiratory syndrome virus from eastern china during 2017–2022
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9606797/
https://www.ncbi.nlm.nih.gov/pubmed/36312912
http://dx.doi.org/10.3389/fmicb.2022.971817
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