Cargando…

The Use of 3D Optical Coherence Tomography to Analyze the Architecture of Cyanobacterial Biofilms Formed on a Carbon Nanotube Composite

The development of environmentally friendly antifouling strategies for marine applications is of paramount importance, and the fabrication of innovative nanocomposite coatings is a promising approach. Moreover, since Optical Coherence Tomography (OCT) is a powerful imaging technique in biofilm scien...

Descripción completa

Detalles Bibliográficos
Autores principales: Romeu, Maria J., Lima, Marta, Gomes, Luciana C., de Jong, Ed. D., Morais, João, Vasconcelos, Vítor, Pereira, Manuel F. R., Soares, Olívia S. G. P., Sjollema, Jelmer, Mergulhão, Filipe J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607013/
https://www.ncbi.nlm.nih.gov/pubmed/36297988
http://dx.doi.org/10.3390/polym14204410
_version_ 1784818434867986432
author Romeu, Maria J.
Lima, Marta
Gomes, Luciana C.
de Jong, Ed. D.
Morais, João
Vasconcelos, Vítor
Pereira, Manuel F. R.
Soares, Olívia S. G. P.
Sjollema, Jelmer
Mergulhão, Filipe J.
author_facet Romeu, Maria J.
Lima, Marta
Gomes, Luciana C.
de Jong, Ed. D.
Morais, João
Vasconcelos, Vítor
Pereira, Manuel F. R.
Soares, Olívia S. G. P.
Sjollema, Jelmer
Mergulhão, Filipe J.
author_sort Romeu, Maria J.
collection PubMed
description The development of environmentally friendly antifouling strategies for marine applications is of paramount importance, and the fabrication of innovative nanocomposite coatings is a promising approach. Moreover, since Optical Coherence Tomography (OCT) is a powerful imaging technique in biofilm science, the improvement of its analytical power is required to better evaluate the biofilm structure under different scenarios. In this study, the effect of carbon nanotube (CNT)-modified surfaces in cyanobacterial biofilm development was assessed over a long-term assay under controlled hydrodynamic conditions. Their impact on the cyanobacterial biofilm architecture was evaluated by novel parameters obtained from three-dimensional (3D) OCT analysis, such as the contour coefficient, total biofilm volume, biovolume, volume of non-connected pores, and the average size of non-connected pores. The results showed that CNTs incorporated into a commercially used epoxy resin (CNT composite) had a higher antifouling effect at the biofilm maturation stage compared to pristine epoxy resin. Along with a delay in biofilm development, a decrease in biofilm wet weight, thickness, and biovolume was also achieved with the CNT composite compared to epoxy resin and glass (control surfaces). Additionally, biofilms developed on the CNT composite were smoother and presented a lower porosity and a strictly packed structure when compared with those formed on the control surfaces. The novel biofilm parameters obtained from 3D OCT imaging are extremely important when evaluating the biofilm architecture and behavior under different scenarios beyond marine applications.
format Online
Article
Text
id pubmed-9607013
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96070132022-10-28 The Use of 3D Optical Coherence Tomography to Analyze the Architecture of Cyanobacterial Biofilms Formed on a Carbon Nanotube Composite Romeu, Maria J. Lima, Marta Gomes, Luciana C. de Jong, Ed. D. Morais, João Vasconcelos, Vítor Pereira, Manuel F. R. Soares, Olívia S. G. P. Sjollema, Jelmer Mergulhão, Filipe J. Polymers (Basel) Article The development of environmentally friendly antifouling strategies for marine applications is of paramount importance, and the fabrication of innovative nanocomposite coatings is a promising approach. Moreover, since Optical Coherence Tomography (OCT) is a powerful imaging technique in biofilm science, the improvement of its analytical power is required to better evaluate the biofilm structure under different scenarios. In this study, the effect of carbon nanotube (CNT)-modified surfaces in cyanobacterial biofilm development was assessed over a long-term assay under controlled hydrodynamic conditions. Their impact on the cyanobacterial biofilm architecture was evaluated by novel parameters obtained from three-dimensional (3D) OCT analysis, such as the contour coefficient, total biofilm volume, biovolume, volume of non-connected pores, and the average size of non-connected pores. The results showed that CNTs incorporated into a commercially used epoxy resin (CNT composite) had a higher antifouling effect at the biofilm maturation stage compared to pristine epoxy resin. Along with a delay in biofilm development, a decrease in biofilm wet weight, thickness, and biovolume was also achieved with the CNT composite compared to epoxy resin and glass (control surfaces). Additionally, biofilms developed on the CNT composite were smoother and presented a lower porosity and a strictly packed structure when compared with those formed on the control surfaces. The novel biofilm parameters obtained from 3D OCT imaging are extremely important when evaluating the biofilm architecture and behavior under different scenarios beyond marine applications. MDPI 2022-10-19 /pmc/articles/PMC9607013/ /pubmed/36297988 http://dx.doi.org/10.3390/polym14204410 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Romeu, Maria J.
Lima, Marta
Gomes, Luciana C.
de Jong, Ed. D.
Morais, João
Vasconcelos, Vítor
Pereira, Manuel F. R.
Soares, Olívia S. G. P.
Sjollema, Jelmer
Mergulhão, Filipe J.
The Use of 3D Optical Coherence Tomography to Analyze the Architecture of Cyanobacterial Biofilms Formed on a Carbon Nanotube Composite
title The Use of 3D Optical Coherence Tomography to Analyze the Architecture of Cyanobacterial Biofilms Formed on a Carbon Nanotube Composite
title_full The Use of 3D Optical Coherence Tomography to Analyze the Architecture of Cyanobacterial Biofilms Formed on a Carbon Nanotube Composite
title_fullStr The Use of 3D Optical Coherence Tomography to Analyze the Architecture of Cyanobacterial Biofilms Formed on a Carbon Nanotube Composite
title_full_unstemmed The Use of 3D Optical Coherence Tomography to Analyze the Architecture of Cyanobacterial Biofilms Formed on a Carbon Nanotube Composite
title_short The Use of 3D Optical Coherence Tomography to Analyze the Architecture of Cyanobacterial Biofilms Formed on a Carbon Nanotube Composite
title_sort use of 3d optical coherence tomography to analyze the architecture of cyanobacterial biofilms formed on a carbon nanotube composite
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607013/
https://www.ncbi.nlm.nih.gov/pubmed/36297988
http://dx.doi.org/10.3390/polym14204410
work_keys_str_mv AT romeumariaj theuseof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT limamarta theuseof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT gomeslucianac theuseof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT dejongedd theuseof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT moraisjoao theuseof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT vasconcelosvitor theuseof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT pereiramanuelfr theuseof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT soaresoliviasgp theuseof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT sjollemajelmer theuseof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT mergulhaofilipej theuseof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT romeumariaj useof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT limamarta useof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT gomeslucianac useof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT dejongedd useof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT moraisjoao useof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT vasconcelosvitor useof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT pereiramanuelfr useof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT soaresoliviasgp useof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT sjollemajelmer useof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite
AT mergulhaofilipej useof3dopticalcoherencetomographytoanalyzethearchitectureofcyanobacterialbiofilmsformedonacarbonnanotubecomposite