Cargando…
Clonal Dissemination of Multidrug-Resistant and Hypervirulent Klebsiella pneumoniae Clonal Complex in a Chinese Hospital
The emergence of high antimicrobial-resistant and hypervirulent Klebsiella pneumoniae (hvKp) clones in clinics has become a cause of concern in recent years. Despite the global spread of the clonal complex (CC) 258, hvKp of other non-CC258 subgroups also emerged. Here, by performing a retrospective...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607059/ https://www.ncbi.nlm.nih.gov/pubmed/36297259 http://dx.doi.org/10.3390/pathogens11101202 |
_version_ | 1784818446844821504 |
---|---|
author | Wang, Yi Hua, Mingxi Wang, Jingqiao Xing, Wen Chen, Jiatian Liu, Jingyuan Du, Pengcheng |
author_facet | Wang, Yi Hua, Mingxi Wang, Jingqiao Xing, Wen Chen, Jiatian Liu, Jingyuan Du, Pengcheng |
author_sort | Wang, Yi |
collection | PubMed |
description | The emergence of high antimicrobial-resistant and hypervirulent Klebsiella pneumoniae (hvKp) clones in clinics has become a cause of concern in recent years. Despite the global spread of the clonal complex (CC) 258, hvKp of other non-CC258 subgroups also emerged. Here, by performing a retrospective study from July 2019 to August 2020 in a Chinese hospital, we obtained 25 K. pneumoniae isolates belonging to CC15. By antimicrobial susceptibility testing and whole genome sequencing and analysis, we obtained the resistant phenotypes and genotypes of these isolates. Twenty-one isolates (84%) were carbapenem-resistant, and eighteen were bla(KPC-2) positive. In addition, ten isolates were identified as putative hvKp and seven were carbapenem-resistant hvKp. Nine isolates carried the pLVPK-like virulence plasmid, which contains the fragment including rmpA2, peg-589, iutA, and iucABCD. Another isolate carried iucA. Phylogenetic analysis revealed that the isolates belonged to four lineages, and the putative hvKp isolates were identified in three of these. Two independent sublineages of putative hvKp were caused by the acquisition of pLVPK-like virulence plasmid. Based on comparative genomic analysis, the number of pairwise single nucleotide polymorphisms amongst the four sublineages, Lineage 1a, 1b, 2a, and 2b, were 1–43, 2–13, 129–279, and 3–4, respectively, indicating clonal transmission of Lineage 1a, 1b, and 2b. These results indicate that multiple lineages of CC15 carbapenem-resistant hvKp have emerged in the hospital and caused nosocomial transmission, and that the spreading of virulence plasmids among classic K. pneumoniae subtypes might become more common and happen more easily. These findings highlight the importance of surveillance of local epidemics of non-CC258 subgroups in hospitals. |
format | Online Article Text |
id | pubmed-9607059 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96070592022-10-28 Clonal Dissemination of Multidrug-Resistant and Hypervirulent Klebsiella pneumoniae Clonal Complex in a Chinese Hospital Wang, Yi Hua, Mingxi Wang, Jingqiao Xing, Wen Chen, Jiatian Liu, Jingyuan Du, Pengcheng Pathogens Article The emergence of high antimicrobial-resistant and hypervirulent Klebsiella pneumoniae (hvKp) clones in clinics has become a cause of concern in recent years. Despite the global spread of the clonal complex (CC) 258, hvKp of other non-CC258 subgroups also emerged. Here, by performing a retrospective study from July 2019 to August 2020 in a Chinese hospital, we obtained 25 K. pneumoniae isolates belonging to CC15. By antimicrobial susceptibility testing and whole genome sequencing and analysis, we obtained the resistant phenotypes and genotypes of these isolates. Twenty-one isolates (84%) were carbapenem-resistant, and eighteen were bla(KPC-2) positive. In addition, ten isolates were identified as putative hvKp and seven were carbapenem-resistant hvKp. Nine isolates carried the pLVPK-like virulence plasmid, which contains the fragment including rmpA2, peg-589, iutA, and iucABCD. Another isolate carried iucA. Phylogenetic analysis revealed that the isolates belonged to four lineages, and the putative hvKp isolates were identified in three of these. Two independent sublineages of putative hvKp were caused by the acquisition of pLVPK-like virulence plasmid. Based on comparative genomic analysis, the number of pairwise single nucleotide polymorphisms amongst the four sublineages, Lineage 1a, 1b, 2a, and 2b, were 1–43, 2–13, 129–279, and 3–4, respectively, indicating clonal transmission of Lineage 1a, 1b, and 2b. These results indicate that multiple lineages of CC15 carbapenem-resistant hvKp have emerged in the hospital and caused nosocomial transmission, and that the spreading of virulence plasmids among classic K. pneumoniae subtypes might become more common and happen more easily. These findings highlight the importance of surveillance of local epidemics of non-CC258 subgroups in hospitals. MDPI 2022-10-18 /pmc/articles/PMC9607059/ /pubmed/36297259 http://dx.doi.org/10.3390/pathogens11101202 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Wang, Yi Hua, Mingxi Wang, Jingqiao Xing, Wen Chen, Jiatian Liu, Jingyuan Du, Pengcheng Clonal Dissemination of Multidrug-Resistant and Hypervirulent Klebsiella pneumoniae Clonal Complex in a Chinese Hospital |
title | Clonal Dissemination of Multidrug-Resistant and Hypervirulent Klebsiella pneumoniae Clonal Complex in a Chinese Hospital |
title_full | Clonal Dissemination of Multidrug-Resistant and Hypervirulent Klebsiella pneumoniae Clonal Complex in a Chinese Hospital |
title_fullStr | Clonal Dissemination of Multidrug-Resistant and Hypervirulent Klebsiella pneumoniae Clonal Complex in a Chinese Hospital |
title_full_unstemmed | Clonal Dissemination of Multidrug-Resistant and Hypervirulent Klebsiella pneumoniae Clonal Complex in a Chinese Hospital |
title_short | Clonal Dissemination of Multidrug-Resistant and Hypervirulent Klebsiella pneumoniae Clonal Complex in a Chinese Hospital |
title_sort | clonal dissemination of multidrug-resistant and hypervirulent klebsiella pneumoniae clonal complex in a chinese hospital |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607059/ https://www.ncbi.nlm.nih.gov/pubmed/36297259 http://dx.doi.org/10.3390/pathogens11101202 |
work_keys_str_mv | AT wangyi clonaldisseminationofmultidrugresistantandhypervirulentklebsiellapneumoniaeclonalcomplexinachinesehospital AT huamingxi clonaldisseminationofmultidrugresistantandhypervirulentklebsiellapneumoniaeclonalcomplexinachinesehospital AT wangjingqiao clonaldisseminationofmultidrugresistantandhypervirulentklebsiellapneumoniaeclonalcomplexinachinesehospital AT xingwen clonaldisseminationofmultidrugresistantandhypervirulentklebsiellapneumoniaeclonalcomplexinachinesehospital AT chenjiatian clonaldisseminationofmultidrugresistantandhypervirulentklebsiellapneumoniaeclonalcomplexinachinesehospital AT liujingyuan clonaldisseminationofmultidrugresistantandhypervirulentklebsiellapneumoniaeclonalcomplexinachinesehospital AT dupengcheng clonaldisseminationofmultidrugresistantandhypervirulentklebsiellapneumoniaeclonalcomplexinachinesehospital |