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The Effects of Resveratrol-Rich Extracts of Vitis vinifera Pruning Waste on HeLa, MCF-7 and MRC-5 Cells: Apoptosis, Autophagia and Necrosis Interplay

Resveratrol is a well-studied plant-derived molecule in cancer biology, with a plethora of documented in vitro effects. However, its low bioavailability and toxicity risk hamper its wider use. In this study, vine shoots after pruning were used as a source of resveratrol (RSV). The activity of subcri...

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Autores principales: Jovanović Galović, Aleksandra, Jovanović Lješković, Nataša, Vidović, Senka, Vladić, Jelena, Jojić, Nikola, Ilić, Milan, Srdić Rajić, Tatjana, Kojić, Vesna, Jakimov, Dimitar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607132/
https://www.ncbi.nlm.nih.gov/pubmed/36297452
http://dx.doi.org/10.3390/pharmaceutics14102017
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author Jovanović Galović, Aleksandra
Jovanović Lješković, Nataša
Vidović, Senka
Vladić, Jelena
Jojić, Nikola
Ilić, Milan
Srdić Rajić, Tatjana
Kojić, Vesna
Jakimov, Dimitar
author_facet Jovanović Galović, Aleksandra
Jovanović Lješković, Nataša
Vidović, Senka
Vladić, Jelena
Jojić, Nikola
Ilić, Milan
Srdić Rajić, Tatjana
Kojić, Vesna
Jakimov, Dimitar
author_sort Jovanović Galović, Aleksandra
collection PubMed
description Resveratrol is a well-studied plant-derived molecule in cancer biology, with a plethora of documented in vitro effects. However, its low bioavailability and toxicity risk hamper its wider use. In this study, vine shoots after pruning were used as a source of resveratrol (RSV). The activity of subcritical water extract (SWE) and dry extract (DE) is examined on three cell lines: HeLa, MCF-7 and MRC-5. The cytotoxic effect is assessed by the MTT test and EB/AO staining, levels of apoptosis are determined by Annexin V assay, autophagia by ULK-1 expression using Western blot and NF-kB activation by p65 ELISA. Our results show that both resveratrol-rich extracts (DE, SWE) have a preferential cytotoxic effect on malignant cell lines (HeLa, MCF-7), and low cytotoxicity on non-malignant cells in culture (MRC-5). Further experiments indicate that the investigated malignant cells undergo different cell death pathways. MCF-7 cells died preferentially by apoptosis, while the HeLa cells died most likely by necrosis (possibly ferroptosis). Protective autophagia is diminished upon treatment with DE in both HeLa and MCF-7 cells, while SWE does not influence the level of autophagia. The extracts are effective even at low concentrations (below IC(50)) in the activation of NF-kB (p65 translocation).
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spelling pubmed-96071322022-10-28 The Effects of Resveratrol-Rich Extracts of Vitis vinifera Pruning Waste on HeLa, MCF-7 and MRC-5 Cells: Apoptosis, Autophagia and Necrosis Interplay Jovanović Galović, Aleksandra Jovanović Lješković, Nataša Vidović, Senka Vladić, Jelena Jojić, Nikola Ilić, Milan Srdić Rajić, Tatjana Kojić, Vesna Jakimov, Dimitar Pharmaceutics Article Resveratrol is a well-studied plant-derived molecule in cancer biology, with a plethora of documented in vitro effects. However, its low bioavailability and toxicity risk hamper its wider use. In this study, vine shoots after pruning were used as a source of resveratrol (RSV). The activity of subcritical water extract (SWE) and dry extract (DE) is examined on three cell lines: HeLa, MCF-7 and MRC-5. The cytotoxic effect is assessed by the MTT test and EB/AO staining, levels of apoptosis are determined by Annexin V assay, autophagia by ULK-1 expression using Western blot and NF-kB activation by p65 ELISA. Our results show that both resveratrol-rich extracts (DE, SWE) have a preferential cytotoxic effect on malignant cell lines (HeLa, MCF-7), and low cytotoxicity on non-malignant cells in culture (MRC-5). Further experiments indicate that the investigated malignant cells undergo different cell death pathways. MCF-7 cells died preferentially by apoptosis, while the HeLa cells died most likely by necrosis (possibly ferroptosis). Protective autophagia is diminished upon treatment with DE in both HeLa and MCF-7 cells, while SWE does not influence the level of autophagia. The extracts are effective even at low concentrations (below IC(50)) in the activation of NF-kB (p65 translocation). MDPI 2022-09-23 /pmc/articles/PMC9607132/ /pubmed/36297452 http://dx.doi.org/10.3390/pharmaceutics14102017 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jovanović Galović, Aleksandra
Jovanović Lješković, Nataša
Vidović, Senka
Vladić, Jelena
Jojić, Nikola
Ilić, Milan
Srdić Rajić, Tatjana
Kojić, Vesna
Jakimov, Dimitar
The Effects of Resveratrol-Rich Extracts of Vitis vinifera Pruning Waste on HeLa, MCF-7 and MRC-5 Cells: Apoptosis, Autophagia and Necrosis Interplay
title The Effects of Resveratrol-Rich Extracts of Vitis vinifera Pruning Waste on HeLa, MCF-7 and MRC-5 Cells: Apoptosis, Autophagia and Necrosis Interplay
title_full The Effects of Resveratrol-Rich Extracts of Vitis vinifera Pruning Waste on HeLa, MCF-7 and MRC-5 Cells: Apoptosis, Autophagia and Necrosis Interplay
title_fullStr The Effects of Resveratrol-Rich Extracts of Vitis vinifera Pruning Waste on HeLa, MCF-7 and MRC-5 Cells: Apoptosis, Autophagia and Necrosis Interplay
title_full_unstemmed The Effects of Resveratrol-Rich Extracts of Vitis vinifera Pruning Waste on HeLa, MCF-7 and MRC-5 Cells: Apoptosis, Autophagia and Necrosis Interplay
title_short The Effects of Resveratrol-Rich Extracts of Vitis vinifera Pruning Waste on HeLa, MCF-7 and MRC-5 Cells: Apoptosis, Autophagia and Necrosis Interplay
title_sort effects of resveratrol-rich extracts of vitis vinifera pruning waste on hela, mcf-7 and mrc-5 cells: apoptosis, autophagia and necrosis interplay
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607132/
https://www.ncbi.nlm.nih.gov/pubmed/36297452
http://dx.doi.org/10.3390/pharmaceutics14102017
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