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Toxicity Effects and Mechanisms of MgO Nanoparticles on the Oomycete Pathogen Phytophthora infestans and Its Host Solanum tuberosum

Engineered nanoparticles have recently been used for innovation in agricultural disease management. However, both the toxicity effects and mechanisms of nanoparticles in target pathogens and their host plants are still largely unknown. Here, we found that magnesium oxide nanoparticles (MgO NPs) coul...

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Autores principales: Wang, Ze-Le, Zhang, Xi, Fan, Guang-Jin, Que, Yi, Xue, Feng, Liu, Ying-Hong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607216/
https://www.ncbi.nlm.nih.gov/pubmed/36287834
http://dx.doi.org/10.3390/toxics10100553
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author Wang, Ze-Le
Zhang, Xi
Fan, Guang-Jin
Que, Yi
Xue, Feng
Liu, Ying-Hong
author_facet Wang, Ze-Le
Zhang, Xi
Fan, Guang-Jin
Que, Yi
Xue, Feng
Liu, Ying-Hong
author_sort Wang, Ze-Le
collection PubMed
description Engineered nanoparticles have recently been used for innovation in agricultural disease management. However, both the toxicity effects and mechanisms of nanoparticles in target pathogens and their host plants are still largely unknown. Here, we found that magnesium oxide nanoparticles (MgO NPs) could protect potatoes against Phytophthora infestans (P. infestans) at a low dosage (50 μg/mL). Through scanning electron microscopy observation, antioxidant enzymes activity measurement, and gene transcriptome analysis, we found that the cell surfaces of P. infestans were destroyed, endogenous superoxide dismutase continuously remained in a higher active state, oxidoreductase activity-related gene ontology (GO) terms were enriched with upregulation, and transporter-activity related GO terms and six essential metabolism-related pathways were enriched with downregulation in P. infestans after 30 min MgO NPs treatment, whereas only 89 genes were changed without enriched GO and pathways terms, and no change in antioxidant activities and phenylalnine ammonialyase in potato appeared at 6 h post-MgO NPs treatment. Only the “plant hormone signal transduction pathway” was enriched with upregulation under differential expression analysis in potatoes. In conclusion, cell surface distortion, continuous oxidative stress, and inhibitions of membrane transport activity and metabolic pathways were toxic mechanisms of Mg ONPs in P. infestans, and the “plant hormone signal transduction pathway” was potentially regulated by Mg-ONPs without obviously harmful effects on potato after Mg ONPs exposure.
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spelling pubmed-96072162022-10-28 Toxicity Effects and Mechanisms of MgO Nanoparticles on the Oomycete Pathogen Phytophthora infestans and Its Host Solanum tuberosum Wang, Ze-Le Zhang, Xi Fan, Guang-Jin Que, Yi Xue, Feng Liu, Ying-Hong Toxics Article Engineered nanoparticles have recently been used for innovation in agricultural disease management. However, both the toxicity effects and mechanisms of nanoparticles in target pathogens and their host plants are still largely unknown. Here, we found that magnesium oxide nanoparticles (MgO NPs) could protect potatoes against Phytophthora infestans (P. infestans) at a low dosage (50 μg/mL). Through scanning electron microscopy observation, antioxidant enzymes activity measurement, and gene transcriptome analysis, we found that the cell surfaces of P. infestans were destroyed, endogenous superoxide dismutase continuously remained in a higher active state, oxidoreductase activity-related gene ontology (GO) terms were enriched with upregulation, and transporter-activity related GO terms and six essential metabolism-related pathways were enriched with downregulation in P. infestans after 30 min MgO NPs treatment, whereas only 89 genes were changed without enriched GO and pathways terms, and no change in antioxidant activities and phenylalnine ammonialyase in potato appeared at 6 h post-MgO NPs treatment. Only the “plant hormone signal transduction pathway” was enriched with upregulation under differential expression analysis in potatoes. In conclusion, cell surface distortion, continuous oxidative stress, and inhibitions of membrane transport activity and metabolic pathways were toxic mechanisms of Mg ONPs in P. infestans, and the “plant hormone signal transduction pathway” was potentially regulated by Mg-ONPs without obviously harmful effects on potato after Mg ONPs exposure. MDPI 2022-09-21 /pmc/articles/PMC9607216/ /pubmed/36287834 http://dx.doi.org/10.3390/toxics10100553 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Ze-Le
Zhang, Xi
Fan, Guang-Jin
Que, Yi
Xue, Feng
Liu, Ying-Hong
Toxicity Effects and Mechanisms of MgO Nanoparticles on the Oomycete Pathogen Phytophthora infestans and Its Host Solanum tuberosum
title Toxicity Effects and Mechanisms of MgO Nanoparticles on the Oomycete Pathogen Phytophthora infestans and Its Host Solanum tuberosum
title_full Toxicity Effects and Mechanisms of MgO Nanoparticles on the Oomycete Pathogen Phytophthora infestans and Its Host Solanum tuberosum
title_fullStr Toxicity Effects and Mechanisms of MgO Nanoparticles on the Oomycete Pathogen Phytophthora infestans and Its Host Solanum tuberosum
title_full_unstemmed Toxicity Effects and Mechanisms of MgO Nanoparticles on the Oomycete Pathogen Phytophthora infestans and Its Host Solanum tuberosum
title_short Toxicity Effects and Mechanisms of MgO Nanoparticles on the Oomycete Pathogen Phytophthora infestans and Its Host Solanum tuberosum
title_sort toxicity effects and mechanisms of mgo nanoparticles on the oomycete pathogen phytophthora infestans and its host solanum tuberosum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607216/
https://www.ncbi.nlm.nih.gov/pubmed/36287834
http://dx.doi.org/10.3390/toxics10100553
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