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Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms

The aim of this study was to evaluate the effect of the Cymbopogon citratus essential oil and its association with chlorhexidine on cariogenic microcosm biofilm composition and acidogenicity. Minimum inhibitory and bactericide concentrations from the essential oil and chlorhexidine were determined b...

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Autores principales: Mouta, Luís Felipe Garcia Leal, Marques, Raquel Souza, Koga-Ito, Cristiane Yumi, Salvador, Marcos José, Giro, Elisa Maria Aparecida, Brighenti, Fernanda Lourenção
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607486/
https://www.ncbi.nlm.nih.gov/pubmed/36297124
http://dx.doi.org/10.3390/pathogens11101067
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author Mouta, Luís Felipe Garcia Leal
Marques, Raquel Souza
Koga-Ito, Cristiane Yumi
Salvador, Marcos José
Giro, Elisa Maria Aparecida
Brighenti, Fernanda Lourenção
author_facet Mouta, Luís Felipe Garcia Leal
Marques, Raquel Souza
Koga-Ito, Cristiane Yumi
Salvador, Marcos José
Giro, Elisa Maria Aparecida
Brighenti, Fernanda Lourenção
author_sort Mouta, Luís Felipe Garcia Leal
collection PubMed
description The aim of this study was to evaluate the effect of the Cymbopogon citratus essential oil and its association with chlorhexidine on cariogenic microcosm biofilm composition and acidogenicity. Minimum inhibitory and bactericide concentrations from the essential oil and chlorhexidine were determined by broth microdilution assay. Microcosms (polymicrobial) biofilms were produced on glass coverslips, using inoculum from human saliva in McBain culture medium (0.5% sucrose exposure for 6 h/day) for 3 days in 24-well plates. The biofilms were treated twice a day and their composition was evaluated by microorganism quantification. The acidogenicity was evaluated by measuring the pH of the spent culture medium in contact with the biofilm. Overall, the association of C. citratus and chlorhexidine reduced total bacterial counts and aciduric bacteria (maximum reduction of 3.55 log UFC/mL) in microcosm biofilms. This group also presented the lowest acidogenicity even when exposed to sucrose-containing medium. C. citratus essential oil increases the effect of digluconate chlorhexidine on microcosm biofilms. Based on these findings, this study can contribute to the development of new formulations that might allow for the use of mouthwashes for a shorter period, which may reduce undesirable effects and increase patient compliance to the treatment.
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spelling pubmed-96074862022-10-28 Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms Mouta, Luís Felipe Garcia Leal Marques, Raquel Souza Koga-Ito, Cristiane Yumi Salvador, Marcos José Giro, Elisa Maria Aparecida Brighenti, Fernanda Lourenção Pathogens Communication The aim of this study was to evaluate the effect of the Cymbopogon citratus essential oil and its association with chlorhexidine on cariogenic microcosm biofilm composition and acidogenicity. Minimum inhibitory and bactericide concentrations from the essential oil and chlorhexidine were determined by broth microdilution assay. Microcosms (polymicrobial) biofilms were produced on glass coverslips, using inoculum from human saliva in McBain culture medium (0.5% sucrose exposure for 6 h/day) for 3 days in 24-well plates. The biofilms were treated twice a day and their composition was evaluated by microorganism quantification. The acidogenicity was evaluated by measuring the pH of the spent culture medium in contact with the biofilm. Overall, the association of C. citratus and chlorhexidine reduced total bacterial counts and aciduric bacteria (maximum reduction of 3.55 log UFC/mL) in microcosm biofilms. This group also presented the lowest acidogenicity even when exposed to sucrose-containing medium. C. citratus essential oil increases the effect of digluconate chlorhexidine on microcosm biofilms. Based on these findings, this study can contribute to the development of new formulations that might allow for the use of mouthwashes for a shorter period, which may reduce undesirable effects and increase patient compliance to the treatment. MDPI 2022-09-20 /pmc/articles/PMC9607486/ /pubmed/36297124 http://dx.doi.org/10.3390/pathogens11101067 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Mouta, Luís Felipe Garcia Leal
Marques, Raquel Souza
Koga-Ito, Cristiane Yumi
Salvador, Marcos José
Giro, Elisa Maria Aparecida
Brighenti, Fernanda Lourenção
Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms
title Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms
title_full Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms
title_fullStr Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms
title_full_unstemmed Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms
title_short Cymbopogon citratus Essential Oil Increases the Effect of Digluconate Chlorhexidine on Microcosm Biofilms
title_sort cymbopogon citratus essential oil increases the effect of digluconate chlorhexidine on microcosm biofilms
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607486/
https://www.ncbi.nlm.nih.gov/pubmed/36297124
http://dx.doi.org/10.3390/pathogens11101067
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