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Preparation and Characterization of PLG Microparticles by the Multiple Emulsion Method for the Sustained Release of Proteins

Rapid release and diminished stability are two of the limitations associated with the growth factors that are essentially used in dental applications. These growth factors are employed to enhance the quality and quantity of tissue or bone matter during regeneration. Therefore, drug delivery devices...

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Autores principales: Yenying, Arphaphat, Tangamatakul, Krissana, Supanchart, Chayarop, Jenvoraphot, Thannaphat, Manokruang, Kiattikhun, Worajittiphon, Patnarin, Punyodom, Winita, Daranarong, Donraporn
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607503/
https://www.ncbi.nlm.nih.gov/pubmed/36296114
http://dx.doi.org/10.3390/mi13101761
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author Yenying, Arphaphat
Tangamatakul, Krissana
Supanchart, Chayarop
Jenvoraphot, Thannaphat
Manokruang, Kiattikhun
Worajittiphon, Patnarin
Punyodom, Winita
Daranarong, Donraporn
author_facet Yenying, Arphaphat
Tangamatakul, Krissana
Supanchart, Chayarop
Jenvoraphot, Thannaphat
Manokruang, Kiattikhun
Worajittiphon, Patnarin
Punyodom, Winita
Daranarong, Donraporn
author_sort Yenying, Arphaphat
collection PubMed
description Rapid release and diminished stability are two of the limitations associated with the growth factors that are essentially used in dental applications. These growth factors are employed to enhance the quality and quantity of tissue or bone matter during regeneration. Therefore, drug delivery devices and systems have been developed to address these limitations. In this study, bovine serum albumin (BSA), as a representative growth factor, was successfully sustained by encapsulation with the medium-absorbable copolymer, poly(L-lactide-co-glycolide) (PLG) 70:30% mol, via the multiple emulsion method. Different PLG, PVA, and BSA concentrations were used to investigate their effects on the BSA encapsulation efficiency. The suitable ratios leading to a better characterization of microparticles and a higher encapsulation efficiency in producing encapsulated PLG microparticles were 8% (w/v) of PLG, 0.25% (w/v) of PVA, and 8% (w/v) of BSA. Furthermore, an in vitro release study revealed a bursting release of BSA from the encapsulated PLG microsphere in the early phase of development. Subsequently, a gradual release was observed over a period of eight weeks. Furthermore, to encapsulate LL-37, different proteins were used in conjunction with PLG under identical conditions with regard to the loading efficiency and morphology, thereby indicating high variations and poor reproducibility. In conclusion, the encapsulated PLG microparticles could effectively protect the protein during encapsulation and could facilitate sustainable protein release over a period of 60 days. Importantly, an optimal method must be employed in order to achieve a high degree of encapsulation efficiency for all of the protein or growth factors. Accordingly, the outcomes of this study will be useful in the manufacture of drug delivery devices that require medium-sustained release growth factors, particularly in dental treatments.
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spelling pubmed-96075032022-10-28 Preparation and Characterization of PLG Microparticles by the Multiple Emulsion Method for the Sustained Release of Proteins Yenying, Arphaphat Tangamatakul, Krissana Supanchart, Chayarop Jenvoraphot, Thannaphat Manokruang, Kiattikhun Worajittiphon, Patnarin Punyodom, Winita Daranarong, Donraporn Micromachines (Basel) Article Rapid release and diminished stability are two of the limitations associated with the growth factors that are essentially used in dental applications. These growth factors are employed to enhance the quality and quantity of tissue or bone matter during regeneration. Therefore, drug delivery devices and systems have been developed to address these limitations. In this study, bovine serum albumin (BSA), as a representative growth factor, was successfully sustained by encapsulation with the medium-absorbable copolymer, poly(L-lactide-co-glycolide) (PLG) 70:30% mol, via the multiple emulsion method. Different PLG, PVA, and BSA concentrations were used to investigate their effects on the BSA encapsulation efficiency. The suitable ratios leading to a better characterization of microparticles and a higher encapsulation efficiency in producing encapsulated PLG microparticles were 8% (w/v) of PLG, 0.25% (w/v) of PVA, and 8% (w/v) of BSA. Furthermore, an in vitro release study revealed a bursting release of BSA from the encapsulated PLG microsphere in the early phase of development. Subsequently, a gradual release was observed over a period of eight weeks. Furthermore, to encapsulate LL-37, different proteins were used in conjunction with PLG under identical conditions with regard to the loading efficiency and morphology, thereby indicating high variations and poor reproducibility. In conclusion, the encapsulated PLG microparticles could effectively protect the protein during encapsulation and could facilitate sustainable protein release over a period of 60 days. Importantly, an optimal method must be employed in order to achieve a high degree of encapsulation efficiency for all of the protein or growth factors. Accordingly, the outcomes of this study will be useful in the manufacture of drug delivery devices that require medium-sustained release growth factors, particularly in dental treatments. MDPI 2022-10-18 /pmc/articles/PMC9607503/ /pubmed/36296114 http://dx.doi.org/10.3390/mi13101761 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Yenying, Arphaphat
Tangamatakul, Krissana
Supanchart, Chayarop
Jenvoraphot, Thannaphat
Manokruang, Kiattikhun
Worajittiphon, Patnarin
Punyodom, Winita
Daranarong, Donraporn
Preparation and Characterization of PLG Microparticles by the Multiple Emulsion Method for the Sustained Release of Proteins
title Preparation and Characterization of PLG Microparticles by the Multiple Emulsion Method for the Sustained Release of Proteins
title_full Preparation and Characterization of PLG Microparticles by the Multiple Emulsion Method for the Sustained Release of Proteins
title_fullStr Preparation and Characterization of PLG Microparticles by the Multiple Emulsion Method for the Sustained Release of Proteins
title_full_unstemmed Preparation and Characterization of PLG Microparticles by the Multiple Emulsion Method for the Sustained Release of Proteins
title_short Preparation and Characterization of PLG Microparticles by the Multiple Emulsion Method for the Sustained Release of Proteins
title_sort preparation and characterization of plg microparticles by the multiple emulsion method for the sustained release of proteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607503/
https://www.ncbi.nlm.nih.gov/pubmed/36296114
http://dx.doi.org/10.3390/mi13101761
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