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Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of Escherichia coli Isolated from Western Lowland Gorilla Faeces (Gorilla gorilla gorilla) of Moukalaba-Doudou National Park (MDNP)

(1) Background: Terrestrial mammals in protected areas have been identified as a potential source of antimicrobial-resistant bacteria. Studies on antimicrobial resistance in gorillas have already been conducted. Thus, this study aimed to describe the phylogroups, pathotypes and prevalence of antimic...

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Autores principales: Oyaba Yinda, Leresche Even Doneilly, Onanga, Richard, Mbehang Nguema, Pierre Philippe, Akomo-Okoue, Etienne François, Nsi Akoue, Gontran, Longo Pendy, Neil Michel, Otsaghe Ekore, Desire, Lendamba, Roméo Wenceslas, Mabika-Mabika, Arsène, Mbeang, Jean Constant Obague, Poungou, Natacha, Ibrahim, Mavoungou, Jacques François, Godreuil, Sylvain
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607589/
https://www.ncbi.nlm.nih.gov/pubmed/36297139
http://dx.doi.org/10.3390/pathogens11101082
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author Oyaba Yinda, Leresche Even Doneilly
Onanga, Richard
Mbehang Nguema, Pierre Philippe
Akomo-Okoue, Etienne François
Nsi Akoue, Gontran
Longo Pendy, Neil Michel
Otsaghe Ekore, Desire
Lendamba, Roméo Wenceslas
Mabika-Mabika, Arsène
Mbeang, Jean Constant Obague
Poungou, Natacha
Ibrahim,
Mavoungou, Jacques François
Godreuil, Sylvain
author_facet Oyaba Yinda, Leresche Even Doneilly
Onanga, Richard
Mbehang Nguema, Pierre Philippe
Akomo-Okoue, Etienne François
Nsi Akoue, Gontran
Longo Pendy, Neil Michel
Otsaghe Ekore, Desire
Lendamba, Roméo Wenceslas
Mabika-Mabika, Arsène
Mbeang, Jean Constant Obague
Poungou, Natacha
Ibrahim,
Mavoungou, Jacques François
Godreuil, Sylvain
author_sort Oyaba Yinda, Leresche Even Doneilly
collection PubMed
description (1) Background: Terrestrial mammals in protected areas have been identified as a potential source of antimicrobial-resistant bacteria. Studies on antimicrobial resistance in gorillas have already been conducted. Thus, this study aimed to describe the phylogroups, pathotypes and prevalence of antimicrobial resistance of Escherichia coli isolated from western lowland gorilla’s faeces living in MDNP. (2) Materials and Methods: Ninety-six faecal samples were collected from western lowland gorillas (Gorilla gorilla gorilla) during daily monitoring in the MDNP. Sixty-four E. coli isolates were obtained and screened for phylogenetic and pathotype group genes by polymerase chain reaction (PCR) after DNA extraction. In addition, antimicrobial susceptibility was determined by the disk diffusion method on Mueller Hinton agar. (3) Results: Sixty-four (64%) isolates of E. coli were obtained from samples. A high level of resistance to the beta-lactam family, a moderate rate for fluoroquinolone and a low rate for aminoglycoside was obtained. All E. coli isolates were positive in phylogroup PCR with a predominance of A (69% ± 11.36%), followed by B2 (20% ± 19.89%) and B1 (10% ± 8.90%) and low prevalence for D (1% ± 3.04%). In addition, twenty E. coli isolates (31%) were positive for pathotype PCR, such as EPEC (85% ± 10.82%) and EPEC/EHEC (15% ± 5.18%) that were obtained in this study. The majority of these MDR E. coli (DECs) belonged to phylogenetic group A, followed by MDR E. coli (DECs) belonging to group B2. (4) Conclusion: This study is the first description of MDR E. coli (DECs) assigned to phylogroup A in western lowland gorillas from the MDNP in Gabon. Thus, wild gorillas in MDNP could be considered as asymptomatic carriers of potential pathogenic MDR E. coli (DECs) that may present a potential risk to human health.
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spelling pubmed-96075892022-10-28 Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of Escherichia coli Isolated from Western Lowland Gorilla Faeces (Gorilla gorilla gorilla) of Moukalaba-Doudou National Park (MDNP) Oyaba Yinda, Leresche Even Doneilly Onanga, Richard Mbehang Nguema, Pierre Philippe Akomo-Okoue, Etienne François Nsi Akoue, Gontran Longo Pendy, Neil Michel Otsaghe Ekore, Desire Lendamba, Roméo Wenceslas Mabika-Mabika, Arsène Mbeang, Jean Constant Obague Poungou, Natacha Ibrahim, Mavoungou, Jacques François Godreuil, Sylvain Pathogens Article (1) Background: Terrestrial mammals in protected areas have been identified as a potential source of antimicrobial-resistant bacteria. Studies on antimicrobial resistance in gorillas have already been conducted. Thus, this study aimed to describe the phylogroups, pathotypes and prevalence of antimicrobial resistance of Escherichia coli isolated from western lowland gorilla’s faeces living in MDNP. (2) Materials and Methods: Ninety-six faecal samples were collected from western lowland gorillas (Gorilla gorilla gorilla) during daily monitoring in the MDNP. Sixty-four E. coli isolates were obtained and screened for phylogenetic and pathotype group genes by polymerase chain reaction (PCR) after DNA extraction. In addition, antimicrobial susceptibility was determined by the disk diffusion method on Mueller Hinton agar. (3) Results: Sixty-four (64%) isolates of E. coli were obtained from samples. A high level of resistance to the beta-lactam family, a moderate rate for fluoroquinolone and a low rate for aminoglycoside was obtained. All E. coli isolates were positive in phylogroup PCR with a predominance of A (69% ± 11.36%), followed by B2 (20% ± 19.89%) and B1 (10% ± 8.90%) and low prevalence for D (1% ± 3.04%). In addition, twenty E. coli isolates (31%) were positive for pathotype PCR, such as EPEC (85% ± 10.82%) and EPEC/EHEC (15% ± 5.18%) that were obtained in this study. The majority of these MDR E. coli (DECs) belonged to phylogenetic group A, followed by MDR E. coli (DECs) belonging to group B2. (4) Conclusion: This study is the first description of MDR E. coli (DECs) assigned to phylogroup A in western lowland gorillas from the MDNP in Gabon. Thus, wild gorillas in MDNP could be considered as asymptomatic carriers of potential pathogenic MDR E. coli (DECs) that may present a potential risk to human health. MDPI 2022-09-23 /pmc/articles/PMC9607589/ /pubmed/36297139 http://dx.doi.org/10.3390/pathogens11101082 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Oyaba Yinda, Leresche Even Doneilly
Onanga, Richard
Mbehang Nguema, Pierre Philippe
Akomo-Okoue, Etienne François
Nsi Akoue, Gontran
Longo Pendy, Neil Michel
Otsaghe Ekore, Desire
Lendamba, Roméo Wenceslas
Mabika-Mabika, Arsène
Mbeang, Jean Constant Obague
Poungou, Natacha
Ibrahim,
Mavoungou, Jacques François
Godreuil, Sylvain
Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of Escherichia coli Isolated from Western Lowland Gorilla Faeces (Gorilla gorilla gorilla) of Moukalaba-Doudou National Park (MDNP)
title Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of Escherichia coli Isolated from Western Lowland Gorilla Faeces (Gorilla gorilla gorilla) of Moukalaba-Doudou National Park (MDNP)
title_full Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of Escherichia coli Isolated from Western Lowland Gorilla Faeces (Gorilla gorilla gorilla) of Moukalaba-Doudou National Park (MDNP)
title_fullStr Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of Escherichia coli Isolated from Western Lowland Gorilla Faeces (Gorilla gorilla gorilla) of Moukalaba-Doudou National Park (MDNP)
title_full_unstemmed Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of Escherichia coli Isolated from Western Lowland Gorilla Faeces (Gorilla gorilla gorilla) of Moukalaba-Doudou National Park (MDNP)
title_short Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of Escherichia coli Isolated from Western Lowland Gorilla Faeces (Gorilla gorilla gorilla) of Moukalaba-Doudou National Park (MDNP)
title_sort phylogenetic groups, pathotypes and antimicrobial resistance of escherichia coli isolated from western lowland gorilla faeces (gorilla gorilla gorilla) of moukalaba-doudou national park (mdnp)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607589/
https://www.ncbi.nlm.nih.gov/pubmed/36297139
http://dx.doi.org/10.3390/pathogens11101082
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