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Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of Escherichia coli Isolated from Western Lowland Gorilla Faeces (Gorilla gorilla gorilla) of Moukalaba-Doudou National Park (MDNP)
(1) Background: Terrestrial mammals in protected areas have been identified as a potential source of antimicrobial-resistant bacteria. Studies on antimicrobial resistance in gorillas have already been conducted. Thus, this study aimed to describe the phylogroups, pathotypes and prevalence of antimic...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607589/ https://www.ncbi.nlm.nih.gov/pubmed/36297139 http://dx.doi.org/10.3390/pathogens11101082 |
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author | Oyaba Yinda, Leresche Even Doneilly Onanga, Richard Mbehang Nguema, Pierre Philippe Akomo-Okoue, Etienne François Nsi Akoue, Gontran Longo Pendy, Neil Michel Otsaghe Ekore, Desire Lendamba, Roméo Wenceslas Mabika-Mabika, Arsène Mbeang, Jean Constant Obague Poungou, Natacha Ibrahim, Mavoungou, Jacques François Godreuil, Sylvain |
author_facet | Oyaba Yinda, Leresche Even Doneilly Onanga, Richard Mbehang Nguema, Pierre Philippe Akomo-Okoue, Etienne François Nsi Akoue, Gontran Longo Pendy, Neil Michel Otsaghe Ekore, Desire Lendamba, Roméo Wenceslas Mabika-Mabika, Arsène Mbeang, Jean Constant Obague Poungou, Natacha Ibrahim, Mavoungou, Jacques François Godreuil, Sylvain |
author_sort | Oyaba Yinda, Leresche Even Doneilly |
collection | PubMed |
description | (1) Background: Terrestrial mammals in protected areas have been identified as a potential source of antimicrobial-resistant bacteria. Studies on antimicrobial resistance in gorillas have already been conducted. Thus, this study aimed to describe the phylogroups, pathotypes and prevalence of antimicrobial resistance of Escherichia coli isolated from western lowland gorilla’s faeces living in MDNP. (2) Materials and Methods: Ninety-six faecal samples were collected from western lowland gorillas (Gorilla gorilla gorilla) during daily monitoring in the MDNP. Sixty-four E. coli isolates were obtained and screened for phylogenetic and pathotype group genes by polymerase chain reaction (PCR) after DNA extraction. In addition, antimicrobial susceptibility was determined by the disk diffusion method on Mueller Hinton agar. (3) Results: Sixty-four (64%) isolates of E. coli were obtained from samples. A high level of resistance to the beta-lactam family, a moderate rate for fluoroquinolone and a low rate for aminoglycoside was obtained. All E. coli isolates were positive in phylogroup PCR with a predominance of A (69% ± 11.36%), followed by B2 (20% ± 19.89%) and B1 (10% ± 8.90%) and low prevalence for D (1% ± 3.04%). In addition, twenty E. coli isolates (31%) were positive for pathotype PCR, such as EPEC (85% ± 10.82%) and EPEC/EHEC (15% ± 5.18%) that were obtained in this study. The majority of these MDR E. coli (DECs) belonged to phylogenetic group A, followed by MDR E. coli (DECs) belonging to group B2. (4) Conclusion: This study is the first description of MDR E. coli (DECs) assigned to phylogroup A in western lowland gorillas from the MDNP in Gabon. Thus, wild gorillas in MDNP could be considered as asymptomatic carriers of potential pathogenic MDR E. coli (DECs) that may present a potential risk to human health. |
format | Online Article Text |
id | pubmed-9607589 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96075892022-10-28 Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of Escherichia coli Isolated from Western Lowland Gorilla Faeces (Gorilla gorilla gorilla) of Moukalaba-Doudou National Park (MDNP) Oyaba Yinda, Leresche Even Doneilly Onanga, Richard Mbehang Nguema, Pierre Philippe Akomo-Okoue, Etienne François Nsi Akoue, Gontran Longo Pendy, Neil Michel Otsaghe Ekore, Desire Lendamba, Roméo Wenceslas Mabika-Mabika, Arsène Mbeang, Jean Constant Obague Poungou, Natacha Ibrahim, Mavoungou, Jacques François Godreuil, Sylvain Pathogens Article (1) Background: Terrestrial mammals in protected areas have been identified as a potential source of antimicrobial-resistant bacteria. Studies on antimicrobial resistance in gorillas have already been conducted. Thus, this study aimed to describe the phylogroups, pathotypes and prevalence of antimicrobial resistance of Escherichia coli isolated from western lowland gorilla’s faeces living in MDNP. (2) Materials and Methods: Ninety-six faecal samples were collected from western lowland gorillas (Gorilla gorilla gorilla) during daily monitoring in the MDNP. Sixty-four E. coli isolates were obtained and screened for phylogenetic and pathotype group genes by polymerase chain reaction (PCR) after DNA extraction. In addition, antimicrobial susceptibility was determined by the disk diffusion method on Mueller Hinton agar. (3) Results: Sixty-four (64%) isolates of E. coli were obtained from samples. A high level of resistance to the beta-lactam family, a moderate rate for fluoroquinolone and a low rate for aminoglycoside was obtained. All E. coli isolates were positive in phylogroup PCR with a predominance of A (69% ± 11.36%), followed by B2 (20% ± 19.89%) and B1 (10% ± 8.90%) and low prevalence for D (1% ± 3.04%). In addition, twenty E. coli isolates (31%) were positive for pathotype PCR, such as EPEC (85% ± 10.82%) and EPEC/EHEC (15% ± 5.18%) that were obtained in this study. The majority of these MDR E. coli (DECs) belonged to phylogenetic group A, followed by MDR E. coli (DECs) belonging to group B2. (4) Conclusion: This study is the first description of MDR E. coli (DECs) assigned to phylogroup A in western lowland gorillas from the MDNP in Gabon. Thus, wild gorillas in MDNP could be considered as asymptomatic carriers of potential pathogenic MDR E. coli (DECs) that may present a potential risk to human health. MDPI 2022-09-23 /pmc/articles/PMC9607589/ /pubmed/36297139 http://dx.doi.org/10.3390/pathogens11101082 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Oyaba Yinda, Leresche Even Doneilly Onanga, Richard Mbehang Nguema, Pierre Philippe Akomo-Okoue, Etienne François Nsi Akoue, Gontran Longo Pendy, Neil Michel Otsaghe Ekore, Desire Lendamba, Roméo Wenceslas Mabika-Mabika, Arsène Mbeang, Jean Constant Obague Poungou, Natacha Ibrahim, Mavoungou, Jacques François Godreuil, Sylvain Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of Escherichia coli Isolated from Western Lowland Gorilla Faeces (Gorilla gorilla gorilla) of Moukalaba-Doudou National Park (MDNP) |
title | Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of Escherichia coli Isolated from Western Lowland Gorilla Faeces (Gorilla gorilla gorilla) of Moukalaba-Doudou National Park (MDNP) |
title_full | Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of Escherichia coli Isolated from Western Lowland Gorilla Faeces (Gorilla gorilla gorilla) of Moukalaba-Doudou National Park (MDNP) |
title_fullStr | Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of Escherichia coli Isolated from Western Lowland Gorilla Faeces (Gorilla gorilla gorilla) of Moukalaba-Doudou National Park (MDNP) |
title_full_unstemmed | Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of Escherichia coli Isolated from Western Lowland Gorilla Faeces (Gorilla gorilla gorilla) of Moukalaba-Doudou National Park (MDNP) |
title_short | Phylogenetic Groups, Pathotypes and Antimicrobial Resistance of Escherichia coli Isolated from Western Lowland Gorilla Faeces (Gorilla gorilla gorilla) of Moukalaba-Doudou National Park (MDNP) |
title_sort | phylogenetic groups, pathotypes and antimicrobial resistance of escherichia coli isolated from western lowland gorilla faeces (gorilla gorilla gorilla) of moukalaba-doudou national park (mdnp) |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607589/ https://www.ncbi.nlm.nih.gov/pubmed/36297139 http://dx.doi.org/10.3390/pathogens11101082 |
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