Microfluidic Technology for the Isolation and Analysis of Exosomes

Exosomes are lipid-bilayer enclosed vesicles with diameters of 30–150 nm, which play a pivotal role in cell communication by transporting their cargoes such as proteins, lipids, and genetic materials. In recent years, exosomes have been under intense investigation, as they show great promise in nume...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Yusong, Wang, Yuqing, Lu, Yanjun, Luo, Xiaomei, Huang, Yinghong, Xie, Ting, Pilarsky, Christian, Dang, Yuanye, Zhang, Jianye
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Review
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607600/
https://www.ncbi.nlm.nih.gov/pubmed/36295924
http://dx.doi.org/10.3390/mi13101571
_version_ 1784818586428112896
author Wu, Yusong
Wang, Yuqing
Lu, Yanjun
Luo, Xiaomei
Huang, Yinghong
Xie, Ting
Pilarsky, Christian
Dang, Yuanye
Zhang, Jianye
author_facet Wu, Yusong
Wang, Yuqing
Lu, Yanjun
Luo, Xiaomei
Huang, Yinghong
Xie, Ting
Pilarsky, Christian
Dang, Yuanye
Zhang, Jianye
author_sort Wu, Yusong
collection PubMed
description Exosomes are lipid-bilayer enclosed vesicles with diameters of 30–150 nm, which play a pivotal role in cell communication by transporting their cargoes such as proteins, lipids, and genetic materials. In recent years, exosomes have been under intense investigation, as they show great promise in numerous areas, especially as bio-markers in liquid biopsies. However, due to the high heterogeneity and the nano size of exosomes, the separation of exosomes is not easy. This review will deliver an outline of the conventional methods and the microfluidic-based technologies for exosome separation. Particular attention is devoted to microfluidic devices, highlighting the efficiency of exosome isolation by these methods. Additionally, this review will introduce advances made in the integrated microfluidics technologies that enable the separation and analysis of exosomes.
format Online
Article
Text
id pubmed-9607600
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96076002022-10-28 Microfluidic Technology for the Isolation and Analysis of Exosomes Wu, Yusong Wang, Yuqing Lu, Yanjun Luo, Xiaomei Huang, Yinghong Xie, Ting Pilarsky, Christian Dang, Yuanye Zhang, Jianye Micromachines (Basel) Review Exosomes are lipid-bilayer enclosed vesicles with diameters of 30–150 nm, which play a pivotal role in cell communication by transporting their cargoes such as proteins, lipids, and genetic materials. In recent years, exosomes have been under intense investigation, as they show great promise in numerous areas, especially as bio-markers in liquid biopsies. However, due to the high heterogeneity and the nano size of exosomes, the separation of exosomes is not easy. This review will deliver an outline of the conventional methods and the microfluidic-based technologies for exosome separation. Particular attention is devoted to microfluidic devices, highlighting the efficiency of exosome isolation by these methods. Additionally, this review will introduce advances made in the integrated microfluidics technologies that enable the separation and analysis of exosomes. MDPI 2022-09-22 /pmc/articles/PMC9607600/ /pubmed/36295924 http://dx.doi.org/10.3390/mi13101571 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Wu, Yusong
Wang, Yuqing
Lu, Yanjun
Luo, Xiaomei
Huang, Yinghong
Xie, Ting
Pilarsky, Christian
Dang, Yuanye
Zhang, Jianye
Microfluidic Technology for the Isolation and Analysis of Exosomes
title Microfluidic Technology for the Isolation and Analysis of Exosomes
title_full Microfluidic Technology for the Isolation and Analysis of Exosomes
title_fullStr Microfluidic Technology for the Isolation and Analysis of Exosomes
title_full_unstemmed Microfluidic Technology for the Isolation and Analysis of Exosomes
title_short Microfluidic Technology for the Isolation and Analysis of Exosomes
title_sort microfluidic technology for the isolation and analysis of exosomes
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607600/
https://www.ncbi.nlm.nih.gov/pubmed/36295924
http://dx.doi.org/10.3390/mi13101571
work_keys_str_mv AT wuyusong microfluidictechnologyfortheisolationandanalysisofexosomes
AT wangyuqing microfluidictechnologyfortheisolationandanalysisofexosomes
AT luyanjun microfluidictechnologyfortheisolationandanalysisofexosomes
AT luoxiaomei microfluidictechnologyfortheisolationandanalysisofexosomes
AT huangyinghong microfluidictechnologyfortheisolationandanalysisofexosomes
AT xieting microfluidictechnologyfortheisolationandanalysisofexosomes
AT pilarskychristian microfluidictechnologyfortheisolationandanalysisofexosomes
AT dangyuanye microfluidictechnologyfortheisolationandanalysisofexosomes
AT zhangjianye microfluidictechnologyfortheisolationandanalysisofexosomes

Ejemplares similares