Cargando…

Injectable Cell-Laden Polysaccharide Hydrogels: In Vivo Evaluation of Cartilage Regeneration

Previously, 5% w/v hyaluronic acid-tyramine (HA-TA) and dextran-tyramine (Dex-TA) enzymatically cross-linked hybrid hydrogels were demonstrated to provide a mechanically stable environment, maintain cell viability, and promote cartilaginous-specific matrix deposition in vitro. In this study, 5% w/v...

Descripción completa

Detalles Bibliográficos
Autores principales: Fu, Yao, Both, Sanne K., Plass, Jacqueline R. M., Dijkstra, Pieter J., Zoetebier, Bram, Karperien, Marcel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607606/
https://www.ncbi.nlm.nih.gov/pubmed/36297870
http://dx.doi.org/10.3390/polym14204292
_version_ 1784818588057600000
author Fu, Yao
Both, Sanne K.
Plass, Jacqueline R. M.
Dijkstra, Pieter J.
Zoetebier, Bram
Karperien, Marcel
author_facet Fu, Yao
Both, Sanne K.
Plass, Jacqueline R. M.
Dijkstra, Pieter J.
Zoetebier, Bram
Karperien, Marcel
author_sort Fu, Yao
collection PubMed
description Previously, 5% w/v hyaluronic acid-tyramine (HA-TA) and dextran-tyramine (Dex-TA) enzymatically cross-linked hybrid hydrogels were demonstrated to provide a mechanically stable environment, maintain cell viability, and promote cartilaginous-specific matrix deposition in vitro. In this study, 5% w/v hybrid hydrogels were combined with human mesenchymal stem cells (hMSCs), bovine chondrocytes (bCHs), or a combination of both in a 4:1 ratio and subcutaneously implanted in the backs of male and female nude rats to assess the performance of cell-laden hydrogels in tissue formation. Subcutaneous implantation of these biomaterials showed signs of integration of the gels within the host tissue. Histological analysis showed residual fibrotic capsules four weeks after implantation. However, enhanced tissue invasion and some giant cell infiltration were observed in the HA-TA/Dex-TA hydrogels laden with either hMSCs or bCHs but not with the co-culture. Moreover, hMSC-bCH co-cultures showed beneficial interaction with the hydrogels, for instance, in enhanced cell proliferation and matrix deposition. In addition, we provide evidence that host gender has an impact on the performance of bCHs encapsulated in HA-TA/Dex-TA hydrogels. This study revealed that hydrogels laden with different types of cells result in distinct host responses. It can be concluded that 5% w/v hydrogels with a higher concentration of Dex-TA (≥50%) laden with bCH-hMSC co-cultures are adequate for injectable applications and in situ cell delivery in cartilage regeneration approaches.
format Online
Article
Text
id pubmed-9607606
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-96076062022-10-28 Injectable Cell-Laden Polysaccharide Hydrogels: In Vivo Evaluation of Cartilage Regeneration Fu, Yao Both, Sanne K. Plass, Jacqueline R. M. Dijkstra, Pieter J. Zoetebier, Bram Karperien, Marcel Polymers (Basel) Article Previously, 5% w/v hyaluronic acid-tyramine (HA-TA) and dextran-tyramine (Dex-TA) enzymatically cross-linked hybrid hydrogels were demonstrated to provide a mechanically stable environment, maintain cell viability, and promote cartilaginous-specific matrix deposition in vitro. In this study, 5% w/v hybrid hydrogels were combined with human mesenchymal stem cells (hMSCs), bovine chondrocytes (bCHs), or a combination of both in a 4:1 ratio and subcutaneously implanted in the backs of male and female nude rats to assess the performance of cell-laden hydrogels in tissue formation. Subcutaneous implantation of these biomaterials showed signs of integration of the gels within the host tissue. Histological analysis showed residual fibrotic capsules four weeks after implantation. However, enhanced tissue invasion and some giant cell infiltration were observed in the HA-TA/Dex-TA hydrogels laden with either hMSCs or bCHs but not with the co-culture. Moreover, hMSC-bCH co-cultures showed beneficial interaction with the hydrogels, for instance, in enhanced cell proliferation and matrix deposition. In addition, we provide evidence that host gender has an impact on the performance of bCHs encapsulated in HA-TA/Dex-TA hydrogels. This study revealed that hydrogels laden with different types of cells result in distinct host responses. It can be concluded that 5% w/v hydrogels with a higher concentration of Dex-TA (≥50%) laden with bCH-hMSC co-cultures are adequate for injectable applications and in situ cell delivery in cartilage regeneration approaches. MDPI 2022-10-12 /pmc/articles/PMC9607606/ /pubmed/36297870 http://dx.doi.org/10.3390/polym14204292 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Fu, Yao
Both, Sanne K.
Plass, Jacqueline R. M.
Dijkstra, Pieter J.
Zoetebier, Bram
Karperien, Marcel
Injectable Cell-Laden Polysaccharide Hydrogels: In Vivo Evaluation of Cartilage Regeneration
title Injectable Cell-Laden Polysaccharide Hydrogels: In Vivo Evaluation of Cartilage Regeneration
title_full Injectable Cell-Laden Polysaccharide Hydrogels: In Vivo Evaluation of Cartilage Regeneration
title_fullStr Injectable Cell-Laden Polysaccharide Hydrogels: In Vivo Evaluation of Cartilage Regeneration
title_full_unstemmed Injectable Cell-Laden Polysaccharide Hydrogels: In Vivo Evaluation of Cartilage Regeneration
title_short Injectable Cell-Laden Polysaccharide Hydrogels: In Vivo Evaluation of Cartilage Regeneration
title_sort injectable cell-laden polysaccharide hydrogels: in vivo evaluation of cartilage regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607606/
https://www.ncbi.nlm.nih.gov/pubmed/36297870
http://dx.doi.org/10.3390/polym14204292
work_keys_str_mv AT fuyao injectablecellladenpolysaccharidehydrogelsinvivoevaluationofcartilageregeneration
AT bothsannek injectablecellladenpolysaccharidehydrogelsinvivoevaluationofcartilageregeneration
AT plassjacquelinerm injectablecellladenpolysaccharidehydrogelsinvivoevaluationofcartilageregeneration
AT dijkstrapieterj injectablecellladenpolysaccharidehydrogelsinvivoevaluationofcartilageregeneration
AT zoetebierbram injectablecellladenpolysaccharidehydrogelsinvivoevaluationofcartilageregeneration
AT karperienmarcel injectablecellladenpolysaccharidehydrogelsinvivoevaluationofcartilageregeneration