Explorative Field Study on the Use of Oral Fluids for the Surveillance of Actinobacillus pleuropneumoniae Infections in Fattening Farms by an Apx-Real-Time PCR

SIMPLE SUMMARY: Oral fluid sampling (OFS) is an animal friendly and easy way for surveillance purposes in domestic swine populations, especially concerning respiratory diseases. In case of Actinobacillus (A.) pleuropneumoniae surveillance, measures are usually combined with burdensome sampling for animals and humans. In the present study, we evaluated the suitability of oral fluids (OFs) for surveillance purposes of A. pleuropneumoniae infections in fattening pigs using an Apx-toxin real-time PCR. We were able to demonstrate that the examination of OFs by an Apx-toxin real-time PCR is suitable for A. pleuropneumoniae surveillance in the field in an animal friendly and easy way. These results might contribute to an increased compliance of laboratory diagnostic measures on pig farms and thereby to increased animal welfare due to less burdensome sampling and improved animal health. ABSTRACT: Oral fluids (OFs) represent a cost effective and reliable tool for surveillance purposes, mostly regarding viruses. In the present study, we evaluated the suitability of OFs for surveillance purposes concerning Actinobacillus (A.) pleuropneumoniae infections in fattening pigs under field conditions. OFs were examined with an Apx-toxin real-time PCR that detects the genes encoding for Apx I-, Apx III-, and Apx IV-toxin. For this purpose, we conducted a pen-wise collection of OFs over one fattening period from fattening pigs of two farms (farm A and B) with a known history of A. pleuropneumoniae infection. Lung lesions were determined at slaughter to estimate the extend of pulmonary lesions and pleural affection. Apx III- and Apx IV-toxin DNA were present in the OFs of both farms whereas Apx I-toxin DNA was present on farm A only. We were able to detect Apx I-, Apx III-, and Apx IV-toxin DNA in different patterns directly after introduction of the new pigs in the farms and over the entire study period. In summary, or results indicate the suitability of OFS for the early detection and surveillance of A. pleuropneumoniae in fattening farms.