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New Approach for Preparing Solid Lipid Nanoparticles with Volatile Oil-Loaded Quercetin Using the Phase-Inversion Temperature Method

Quercetin (QCT), a natural flavonoid, is of research interest owing to its pharmacological properties. However, its pharmacokinetic limitations could hinder its widespread therapeutic use. Nanocarriers, especially solid lipid nanoparticles (SLNs), might overcome this constraint. This study aimed to...

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Autores principales: Weerapol, Yotsanan, Manmuan, Suwisit, Chaothanaphat, Nattaya, Limmatvapirat, Sontaya, Sirirak, Jitnapa, Tamdee, Poomipat, Tubtimsri, Sukannika
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607647/
https://www.ncbi.nlm.nih.gov/pubmed/36297420
http://dx.doi.org/10.3390/pharmaceutics14101984
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author Weerapol, Yotsanan
Manmuan, Suwisit
Chaothanaphat, Nattaya
Limmatvapirat, Sontaya
Sirirak, Jitnapa
Tamdee, Poomipat
Tubtimsri, Sukannika
author_facet Weerapol, Yotsanan
Manmuan, Suwisit
Chaothanaphat, Nattaya
Limmatvapirat, Sontaya
Sirirak, Jitnapa
Tamdee, Poomipat
Tubtimsri, Sukannika
author_sort Weerapol, Yotsanan
collection PubMed
description Quercetin (QCT), a natural flavonoid, is of research interest owing to its pharmacological properties. However, its pharmacokinetic limitations could hinder its widespread therapeutic use. Nanocarriers, especially solid lipid nanoparticles (SLNs), might overcome this constraint. This study aimed to investigate QCT-loaded SLNs prepared via a new approach using a volatile oil. The phase-inversion temperature method was used to incorporate rosemary oil (RMO) into SLNs prepared using solid lipids possessing different chemical structures. Among the solid lipids used in the formulations, trilaurin (TLR) exhibited the smallest particle size and good stability after a temperature cycling test. SLNs prepared with a ratio of RMO to TLR of 1:3 could load QCT with an entrapment efficiency of >60% and drug loading of ~2% w/w. The smallest particle size was achieved using the polyoxyethylene-hydrogenated castor oil RH40, and the particle size depended on the concentration. The drug-release profile of QCT_TLR exhibited prolonged biphasic release for >24 h. QCT_TLR was a safe formulation, as indicated by a cell viability percentage of >75% at <2% v/v. In a computer simulation, the system with RMO enabled smaller sized SLNs than those without RMO. This new discovery shows great promise for producing SLNs via the phase-inversion temperature method with incorporation of volatile oil, particularly for delivering compounds with limited water solubility.
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spelling pubmed-96076472022-10-28 New Approach for Preparing Solid Lipid Nanoparticles with Volatile Oil-Loaded Quercetin Using the Phase-Inversion Temperature Method Weerapol, Yotsanan Manmuan, Suwisit Chaothanaphat, Nattaya Limmatvapirat, Sontaya Sirirak, Jitnapa Tamdee, Poomipat Tubtimsri, Sukannika Pharmaceutics Article Quercetin (QCT), a natural flavonoid, is of research interest owing to its pharmacological properties. However, its pharmacokinetic limitations could hinder its widespread therapeutic use. Nanocarriers, especially solid lipid nanoparticles (SLNs), might overcome this constraint. This study aimed to investigate QCT-loaded SLNs prepared via a new approach using a volatile oil. The phase-inversion temperature method was used to incorporate rosemary oil (RMO) into SLNs prepared using solid lipids possessing different chemical structures. Among the solid lipids used in the formulations, trilaurin (TLR) exhibited the smallest particle size and good stability after a temperature cycling test. SLNs prepared with a ratio of RMO to TLR of 1:3 could load QCT with an entrapment efficiency of >60% and drug loading of ~2% w/w. The smallest particle size was achieved using the polyoxyethylene-hydrogenated castor oil RH40, and the particle size depended on the concentration. The drug-release profile of QCT_TLR exhibited prolonged biphasic release for >24 h. QCT_TLR was a safe formulation, as indicated by a cell viability percentage of >75% at <2% v/v. In a computer simulation, the system with RMO enabled smaller sized SLNs than those without RMO. This new discovery shows great promise for producing SLNs via the phase-inversion temperature method with incorporation of volatile oil, particularly for delivering compounds with limited water solubility. MDPI 2022-09-20 /pmc/articles/PMC9607647/ /pubmed/36297420 http://dx.doi.org/10.3390/pharmaceutics14101984 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Weerapol, Yotsanan
Manmuan, Suwisit
Chaothanaphat, Nattaya
Limmatvapirat, Sontaya
Sirirak, Jitnapa
Tamdee, Poomipat
Tubtimsri, Sukannika
New Approach for Preparing Solid Lipid Nanoparticles with Volatile Oil-Loaded Quercetin Using the Phase-Inversion Temperature Method
title New Approach for Preparing Solid Lipid Nanoparticles with Volatile Oil-Loaded Quercetin Using the Phase-Inversion Temperature Method
title_full New Approach for Preparing Solid Lipid Nanoparticles with Volatile Oil-Loaded Quercetin Using the Phase-Inversion Temperature Method
title_fullStr New Approach for Preparing Solid Lipid Nanoparticles with Volatile Oil-Loaded Quercetin Using the Phase-Inversion Temperature Method
title_full_unstemmed New Approach for Preparing Solid Lipid Nanoparticles with Volatile Oil-Loaded Quercetin Using the Phase-Inversion Temperature Method
title_short New Approach for Preparing Solid Lipid Nanoparticles with Volatile Oil-Loaded Quercetin Using the Phase-Inversion Temperature Method
title_sort new approach for preparing solid lipid nanoparticles with volatile oil-loaded quercetin using the phase-inversion temperature method
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607647/
https://www.ncbi.nlm.nih.gov/pubmed/36297420
http://dx.doi.org/10.3390/pharmaceutics14101984
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