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An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection

Chronic human liver fluke infections caused by Opisthorchis viverrini and Clonorchis sinensis can last for decades and cause liver and biliary diseases, including life-threatening pathology prior to cholangiocarcinoma (CCA). CCA generally has a poor prognosis. Serological diagnosis can support paras...

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Autores principales: Sadaow, Lakkhana, Rodpai, Rutchanee, Janwan, Penchom, Boonroumkaew, Patcharaporn, Sanpool, Oranuch, Thanchomnang, Tongjit, Yamasaki, Hiroshi, Ittiprasert, Wannaporn, Mann, Victoria H., Brindley, Paul J., Maleewong, Wanchai, Intapan, Pewpan M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607866/
https://www.ncbi.nlm.nih.gov/pubmed/36288049
http://dx.doi.org/10.3390/tropicalmed7100308
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author Sadaow, Lakkhana
Rodpai, Rutchanee
Janwan, Penchom
Boonroumkaew, Patcharaporn
Sanpool, Oranuch
Thanchomnang, Tongjit
Yamasaki, Hiroshi
Ittiprasert, Wannaporn
Mann, Victoria H.
Brindley, Paul J.
Maleewong, Wanchai
Intapan, Pewpan M.
author_facet Sadaow, Lakkhana
Rodpai, Rutchanee
Janwan, Penchom
Boonroumkaew, Patcharaporn
Sanpool, Oranuch
Thanchomnang, Tongjit
Yamasaki, Hiroshi
Ittiprasert, Wannaporn
Mann, Victoria H.
Brindley, Paul J.
Maleewong, Wanchai
Intapan, Pewpan M.
author_sort Sadaow, Lakkhana
collection PubMed
description Chronic human liver fluke infections caused by Opisthorchis viverrini and Clonorchis sinensis can last for decades and cause liver and biliary diseases, including life-threatening pathology prior to cholangiocarcinoma (CCA). CCA generally has a poor prognosis. Serological diagnosis can support parasitological examination in diagnosing disease and screening for the risk of CCA. Here, we present an improved and innovative lateral flow immunochromatographic test (ICT) kit that uses whole-blood samples (WBS) rather than serum to diagnose human opisthorchiasis, which also successfully diagnosed human clonorchiasis. This ICT includes a soluble worm extract of O. viverrini adults and colloidal-gold-labeled conjugates of the IgG antibody to evaluate the diagnostic values with simulated WBS (n = 347). Simulated WBS were obtained by the spiking infection sera with red blood cells. The diagnostic sensitivity, specificity, positive and negative predictive values, and accuracy for detecting opisthorchiasis were 95.5%, 87.0%, 80.5%, 97.2%, and 90.1%, respectively. For clonorchiasis, these findings were 85.7%, 87.0%, 53.6%, 97.2%, and 86.8%, respectively. Combined for both diseases, they were 93.2%, 87.0%, 84.0%, 94.6%, and 89.6%, respectively. The ICT kit can possibly replace the ICT platforms for antibody detection in serum samples in field surveys in remote areas where sophisticated equipment is not available.
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spelling pubmed-96078662022-10-28 An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection Sadaow, Lakkhana Rodpai, Rutchanee Janwan, Penchom Boonroumkaew, Patcharaporn Sanpool, Oranuch Thanchomnang, Tongjit Yamasaki, Hiroshi Ittiprasert, Wannaporn Mann, Victoria H. Brindley, Paul J. Maleewong, Wanchai Intapan, Pewpan M. Trop Med Infect Dis Article Chronic human liver fluke infections caused by Opisthorchis viverrini and Clonorchis sinensis can last for decades and cause liver and biliary diseases, including life-threatening pathology prior to cholangiocarcinoma (CCA). CCA generally has a poor prognosis. Serological diagnosis can support parasitological examination in diagnosing disease and screening for the risk of CCA. Here, we present an improved and innovative lateral flow immunochromatographic test (ICT) kit that uses whole-blood samples (WBS) rather than serum to diagnose human opisthorchiasis, which also successfully diagnosed human clonorchiasis. This ICT includes a soluble worm extract of O. viverrini adults and colloidal-gold-labeled conjugates of the IgG antibody to evaluate the diagnostic values with simulated WBS (n = 347). Simulated WBS were obtained by the spiking infection sera with red blood cells. The diagnostic sensitivity, specificity, positive and negative predictive values, and accuracy for detecting opisthorchiasis were 95.5%, 87.0%, 80.5%, 97.2%, and 90.1%, respectively. For clonorchiasis, these findings were 85.7%, 87.0%, 53.6%, 97.2%, and 86.8%, respectively. Combined for both diseases, they were 93.2%, 87.0%, 84.0%, 94.6%, and 89.6%, respectively. The ICT kit can possibly replace the ICT platforms for antibody detection in serum samples in field surveys in remote areas where sophisticated equipment is not available. MDPI 2022-10-17 /pmc/articles/PMC9607866/ /pubmed/36288049 http://dx.doi.org/10.3390/tropicalmed7100308 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sadaow, Lakkhana
Rodpai, Rutchanee
Janwan, Penchom
Boonroumkaew, Patcharaporn
Sanpool, Oranuch
Thanchomnang, Tongjit
Yamasaki, Hiroshi
Ittiprasert, Wannaporn
Mann, Victoria H.
Brindley, Paul J.
Maleewong, Wanchai
Intapan, Pewpan M.
An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection
title An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection
title_full An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection
title_fullStr An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection
title_full_unstemmed An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection
title_short An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection
title_sort innovative test for the rapid detection of specific igg antibodies in human whole-blood for the diagnosis of opisthorchis viverrini infection
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607866/
https://www.ncbi.nlm.nih.gov/pubmed/36288049
http://dx.doi.org/10.3390/tropicalmed7100308
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