Cargando…
An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection
Chronic human liver fluke infections caused by Opisthorchis viverrini and Clonorchis sinensis can last for decades and cause liver and biliary diseases, including life-threatening pathology prior to cholangiocarcinoma (CCA). CCA generally has a poor prognosis. Serological diagnosis can support paras...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607866/ https://www.ncbi.nlm.nih.gov/pubmed/36288049 http://dx.doi.org/10.3390/tropicalmed7100308 |
_version_ | 1784818647373447168 |
---|---|
author | Sadaow, Lakkhana Rodpai, Rutchanee Janwan, Penchom Boonroumkaew, Patcharaporn Sanpool, Oranuch Thanchomnang, Tongjit Yamasaki, Hiroshi Ittiprasert, Wannaporn Mann, Victoria H. Brindley, Paul J. Maleewong, Wanchai Intapan, Pewpan M. |
author_facet | Sadaow, Lakkhana Rodpai, Rutchanee Janwan, Penchom Boonroumkaew, Patcharaporn Sanpool, Oranuch Thanchomnang, Tongjit Yamasaki, Hiroshi Ittiprasert, Wannaporn Mann, Victoria H. Brindley, Paul J. Maleewong, Wanchai Intapan, Pewpan M. |
author_sort | Sadaow, Lakkhana |
collection | PubMed |
description | Chronic human liver fluke infections caused by Opisthorchis viverrini and Clonorchis sinensis can last for decades and cause liver and biliary diseases, including life-threatening pathology prior to cholangiocarcinoma (CCA). CCA generally has a poor prognosis. Serological diagnosis can support parasitological examination in diagnosing disease and screening for the risk of CCA. Here, we present an improved and innovative lateral flow immunochromatographic test (ICT) kit that uses whole-blood samples (WBS) rather than serum to diagnose human opisthorchiasis, which also successfully diagnosed human clonorchiasis. This ICT includes a soluble worm extract of O. viverrini adults and colloidal-gold-labeled conjugates of the IgG antibody to evaluate the diagnostic values with simulated WBS (n = 347). Simulated WBS were obtained by the spiking infection sera with red blood cells. The diagnostic sensitivity, specificity, positive and negative predictive values, and accuracy for detecting opisthorchiasis were 95.5%, 87.0%, 80.5%, 97.2%, and 90.1%, respectively. For clonorchiasis, these findings were 85.7%, 87.0%, 53.6%, 97.2%, and 86.8%, respectively. Combined for both diseases, they were 93.2%, 87.0%, 84.0%, 94.6%, and 89.6%, respectively. The ICT kit can possibly replace the ICT platforms for antibody detection in serum samples in field surveys in remote areas where sophisticated equipment is not available. |
format | Online Article Text |
id | pubmed-9607866 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-96078662022-10-28 An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection Sadaow, Lakkhana Rodpai, Rutchanee Janwan, Penchom Boonroumkaew, Patcharaporn Sanpool, Oranuch Thanchomnang, Tongjit Yamasaki, Hiroshi Ittiprasert, Wannaporn Mann, Victoria H. Brindley, Paul J. Maleewong, Wanchai Intapan, Pewpan M. Trop Med Infect Dis Article Chronic human liver fluke infections caused by Opisthorchis viverrini and Clonorchis sinensis can last for decades and cause liver and biliary diseases, including life-threatening pathology prior to cholangiocarcinoma (CCA). CCA generally has a poor prognosis. Serological diagnosis can support parasitological examination in diagnosing disease and screening for the risk of CCA. Here, we present an improved and innovative lateral flow immunochromatographic test (ICT) kit that uses whole-blood samples (WBS) rather than serum to diagnose human opisthorchiasis, which also successfully diagnosed human clonorchiasis. This ICT includes a soluble worm extract of O. viverrini adults and colloidal-gold-labeled conjugates of the IgG antibody to evaluate the diagnostic values with simulated WBS (n = 347). Simulated WBS were obtained by the spiking infection sera with red blood cells. The diagnostic sensitivity, specificity, positive and negative predictive values, and accuracy for detecting opisthorchiasis were 95.5%, 87.0%, 80.5%, 97.2%, and 90.1%, respectively. For clonorchiasis, these findings were 85.7%, 87.0%, 53.6%, 97.2%, and 86.8%, respectively. Combined for both diseases, they were 93.2%, 87.0%, 84.0%, 94.6%, and 89.6%, respectively. The ICT kit can possibly replace the ICT platforms for antibody detection in serum samples in field surveys in remote areas where sophisticated equipment is not available. MDPI 2022-10-17 /pmc/articles/PMC9607866/ /pubmed/36288049 http://dx.doi.org/10.3390/tropicalmed7100308 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Sadaow, Lakkhana Rodpai, Rutchanee Janwan, Penchom Boonroumkaew, Patcharaporn Sanpool, Oranuch Thanchomnang, Tongjit Yamasaki, Hiroshi Ittiprasert, Wannaporn Mann, Victoria H. Brindley, Paul J. Maleewong, Wanchai Intapan, Pewpan M. An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection |
title | An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection |
title_full | An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection |
title_fullStr | An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection |
title_full_unstemmed | An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection |
title_short | An Innovative Test for the Rapid Detection of Specific IgG Antibodies in Human Whole-Blood for the Diagnosis of Opisthorchis viverrini Infection |
title_sort | innovative test for the rapid detection of specific igg antibodies in human whole-blood for the diagnosis of opisthorchis viverrini infection |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607866/ https://www.ncbi.nlm.nih.gov/pubmed/36288049 http://dx.doi.org/10.3390/tropicalmed7100308 |
work_keys_str_mv | AT sadaowlakkhana aninnovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT rodpairutchanee aninnovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT janwanpenchom aninnovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT boonroumkaewpatcharaporn aninnovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT sanpooloranuch aninnovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT thanchomnangtongjit aninnovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT yamasakihiroshi aninnovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT ittiprasertwannaporn aninnovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT mannvictoriah aninnovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT brindleypaulj aninnovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT maleewongwanchai aninnovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT intapanpewpanm aninnovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT sadaowlakkhana innovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT rodpairutchanee innovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT janwanpenchom innovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT boonroumkaewpatcharaporn innovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT sanpooloranuch innovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT thanchomnangtongjit innovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT yamasakihiroshi innovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT ittiprasertwannaporn innovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT mannvictoriah innovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT brindleypaulj innovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT maleewongwanchai innovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection AT intapanpewpanm innovativetestfortherapiddetectionofspecificiggantibodiesinhumanwholebloodforthediagnosisofopisthorchisviverriniinfection |