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Use of Antigen Combinations to Address Complex Leishmania-Seropositivity Patterns in Dogs Living in Canine Leishmaniosis Endemic Regions of Portugal

Canine leishmaniosis (CanL) is a vector-borne disease caused by Leishmania infantum. Infection in dogs can result in a disease with non-specific clinical signs or in a subclinical condition. Infection diagnosis is crucial to guide public health measures considering the zoonotic potential of L. infan...

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Autores principales: Lima, Carla Silva, Esteves, Sofia, Costa, Inês, Brancal, Hugo, Lima, Clara, Amorim, Célia, Cardoso, Luís, Santarém, Nuno, Cordeiro-da-Silva, Anabela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607924/
https://www.ncbi.nlm.nih.gov/pubmed/36296294
http://dx.doi.org/10.3390/microorganisms10102018
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author Lima, Carla Silva
Esteves, Sofia
Costa, Inês
Brancal, Hugo
Lima, Clara
Amorim, Célia
Cardoso, Luís
Santarém, Nuno
Cordeiro-da-Silva, Anabela
author_facet Lima, Carla Silva
Esteves, Sofia
Costa, Inês
Brancal, Hugo
Lima, Clara
Amorim, Célia
Cardoso, Luís
Santarém, Nuno
Cordeiro-da-Silva, Anabela
author_sort Lima, Carla Silva
collection PubMed
description Canine leishmaniosis (CanL) is a vector-borne disease caused by Leishmania infantum. Infection in dogs can result in a disease with non-specific clinical signs or in a subclinical condition. Infection diagnosis is crucial to guide public health measures considering the zoonotic potential of L. infantum. Serological approaches to detect infection with a reduced antigen panel potentially limit the quality of the information obtained. To evaluate the impact of using distinct antigens in a serological survey, a cohort with 390 dogs from endemic regions in Portugal was subjected to a serological evaluation using ELISA and DAT. Using ELISA, six Leishmania-specific antigens in conjunction with a non-related antigen, Escherichia coli soluble antigens, were evaluated. The global seroprevalence was 10.5% for DAT and 15.4 to 23.1% for ELISA, depending on the antigen for the latter. Still, only 8.2% of the animals were seropositive to all Leishmania-specific antigens. Importantly, a further 31.0% presented antigen-dependent seropositivity. Considering this observation, a serological score system was proposed and validated to address the complex serology results. With this system, the overall dog seropositivity was 26.9%. This work highlights the limitations of single-antigen serological surveys and presents an approach that might contribute to the establishment of CanL-specific serological profiles.
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spelling pubmed-96079242022-10-28 Use of Antigen Combinations to Address Complex Leishmania-Seropositivity Patterns in Dogs Living in Canine Leishmaniosis Endemic Regions of Portugal Lima, Carla Silva Esteves, Sofia Costa, Inês Brancal, Hugo Lima, Clara Amorim, Célia Cardoso, Luís Santarém, Nuno Cordeiro-da-Silva, Anabela Microorganisms Article Canine leishmaniosis (CanL) is a vector-borne disease caused by Leishmania infantum. Infection in dogs can result in a disease with non-specific clinical signs or in a subclinical condition. Infection diagnosis is crucial to guide public health measures considering the zoonotic potential of L. infantum. Serological approaches to detect infection with a reduced antigen panel potentially limit the quality of the information obtained. To evaluate the impact of using distinct antigens in a serological survey, a cohort with 390 dogs from endemic regions in Portugal was subjected to a serological evaluation using ELISA and DAT. Using ELISA, six Leishmania-specific antigens in conjunction with a non-related antigen, Escherichia coli soluble antigens, were evaluated. The global seroprevalence was 10.5% for DAT and 15.4 to 23.1% for ELISA, depending on the antigen for the latter. Still, only 8.2% of the animals were seropositive to all Leishmania-specific antigens. Importantly, a further 31.0% presented antigen-dependent seropositivity. Considering this observation, a serological score system was proposed and validated to address the complex serology results. With this system, the overall dog seropositivity was 26.9%. This work highlights the limitations of single-antigen serological surveys and presents an approach that might contribute to the establishment of CanL-specific serological profiles. MDPI 2022-10-12 /pmc/articles/PMC9607924/ /pubmed/36296294 http://dx.doi.org/10.3390/microorganisms10102018 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lima, Carla Silva
Esteves, Sofia
Costa, Inês
Brancal, Hugo
Lima, Clara
Amorim, Célia
Cardoso, Luís
Santarém, Nuno
Cordeiro-da-Silva, Anabela
Use of Antigen Combinations to Address Complex Leishmania-Seropositivity Patterns in Dogs Living in Canine Leishmaniosis Endemic Regions of Portugal
title Use of Antigen Combinations to Address Complex Leishmania-Seropositivity Patterns in Dogs Living in Canine Leishmaniosis Endemic Regions of Portugal
title_full Use of Antigen Combinations to Address Complex Leishmania-Seropositivity Patterns in Dogs Living in Canine Leishmaniosis Endemic Regions of Portugal
title_fullStr Use of Antigen Combinations to Address Complex Leishmania-Seropositivity Patterns in Dogs Living in Canine Leishmaniosis Endemic Regions of Portugal
title_full_unstemmed Use of Antigen Combinations to Address Complex Leishmania-Seropositivity Patterns in Dogs Living in Canine Leishmaniosis Endemic Regions of Portugal
title_short Use of Antigen Combinations to Address Complex Leishmania-Seropositivity Patterns in Dogs Living in Canine Leishmaniosis Endemic Regions of Portugal
title_sort use of antigen combinations to address complex leishmania-seropositivity patterns in dogs living in canine leishmaniosis endemic regions of portugal
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9607924/
https://www.ncbi.nlm.nih.gov/pubmed/36296294
http://dx.doi.org/10.3390/microorganisms10102018
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