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Molecular Changes Following Induction of Hepatocellular Carcinoma by Diethylnitrosamine and Thioacetamide, and Subsequent Treatment with Dioscorea membranacea Extract
Hepatocellular carcinoma (HCC) is a primary liver cancer commonly found in adults. Previously, we showed the anticancer effects of Thai herbal plant extract, Dioscorea membranacea Pierre (DM), in HCC-bearing rats. In the present study, we further examined the proposed mechanism of DM, including apop...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Ivyspring International Publisher
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608040/ https://www.ncbi.nlm.nih.gov/pubmed/36313224 http://dx.doi.org/10.7150/ijms.72987 |
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author | Kerdput, Vichununt Kanjanapongkul, Kritsakorn Itharat, Arunporn Pramong, Ratchadaporn Lamers, Wouter H Hakvoort, Theodorus B. M. Jongejan, Aldo Pradidarcheep, Wisuit |
author_facet | Kerdput, Vichununt Kanjanapongkul, Kritsakorn Itharat, Arunporn Pramong, Ratchadaporn Lamers, Wouter H Hakvoort, Theodorus B. M. Jongejan, Aldo Pradidarcheep, Wisuit |
author_sort | Kerdput, Vichununt |
collection | PubMed |
description | Hepatocellular carcinoma (HCC) is a primary liver cancer commonly found in adults. Previously, we showed the anticancer effects of Thai herbal plant extract, Dioscorea membranacea Pierre (DM), in HCC-bearing rats. In the present study, we further examined the proposed mechanism of DM, including apoptosis and antioxidant activity. Moreover, we used RNA sequencing (RNA-seq) to analyze molecular pathways in the rat model in which HCC was induced by diethylnitrosamine (DEN) and thioacetamide (TAA). The HCC-bearing rats were then treated with 40 mg/kg of DM for 8 weeks, after which experimental and control rats were sacrificed and liver tissues were collected. The RNA-seq data of DEN/TAA-treated rats exhibited upregulation of 16 hallmark pathways, including epithelial mesenchymal transition, inflammatory responses, and angiogenesis (p<0.01). DM extract expanded the Bax protein-positive pericentral zone in the tumor areas and decreased hepatic malondialdehyde levels, implying a decrease in lipid peroxidation in liver. However, DM treatment did not ameliorate the molecular pathways induced in DEN/TAA-treated livers. Our findings indicate that DM extract has antioxidant activity and exerts its pro-apoptotic effect on rat HCCs in vivo at the (post-)translational level. |
format | Online Article Text |
id | pubmed-9608040 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Ivyspring International Publisher |
record_format | MEDLINE/PubMed |
spelling | pubmed-96080402022-10-28 Molecular Changes Following Induction of Hepatocellular Carcinoma by Diethylnitrosamine and Thioacetamide, and Subsequent Treatment with Dioscorea membranacea Extract Kerdput, Vichununt Kanjanapongkul, Kritsakorn Itharat, Arunporn Pramong, Ratchadaporn Lamers, Wouter H Hakvoort, Theodorus B. M. Jongejan, Aldo Pradidarcheep, Wisuit Int J Med Sci Research Paper Hepatocellular carcinoma (HCC) is a primary liver cancer commonly found in adults. Previously, we showed the anticancer effects of Thai herbal plant extract, Dioscorea membranacea Pierre (DM), in HCC-bearing rats. In the present study, we further examined the proposed mechanism of DM, including apoptosis and antioxidant activity. Moreover, we used RNA sequencing (RNA-seq) to analyze molecular pathways in the rat model in which HCC was induced by diethylnitrosamine (DEN) and thioacetamide (TAA). The HCC-bearing rats were then treated with 40 mg/kg of DM for 8 weeks, after which experimental and control rats were sacrificed and liver tissues were collected. The RNA-seq data of DEN/TAA-treated rats exhibited upregulation of 16 hallmark pathways, including epithelial mesenchymal transition, inflammatory responses, and angiogenesis (p<0.01). DM extract expanded the Bax protein-positive pericentral zone in the tumor areas and decreased hepatic malondialdehyde levels, implying a decrease in lipid peroxidation in liver. However, DM treatment did not ameliorate the molecular pathways induced in DEN/TAA-treated livers. Our findings indicate that DM extract has antioxidant activity and exerts its pro-apoptotic effect on rat HCCs in vivo at the (post-)translational level. Ivyspring International Publisher 2022-10-09 /pmc/articles/PMC9608040/ /pubmed/36313224 http://dx.doi.org/10.7150/ijms.72987 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions. |
spellingShingle | Research Paper Kerdput, Vichununt Kanjanapongkul, Kritsakorn Itharat, Arunporn Pramong, Ratchadaporn Lamers, Wouter H Hakvoort, Theodorus B. M. Jongejan, Aldo Pradidarcheep, Wisuit Molecular Changes Following Induction of Hepatocellular Carcinoma by Diethylnitrosamine and Thioacetamide, and Subsequent Treatment with Dioscorea membranacea Extract |
title | Molecular Changes Following Induction of Hepatocellular Carcinoma by Diethylnitrosamine and Thioacetamide, and Subsequent Treatment with Dioscorea membranacea Extract |
title_full | Molecular Changes Following Induction of Hepatocellular Carcinoma by Diethylnitrosamine and Thioacetamide, and Subsequent Treatment with Dioscorea membranacea Extract |
title_fullStr | Molecular Changes Following Induction of Hepatocellular Carcinoma by Diethylnitrosamine and Thioacetamide, and Subsequent Treatment with Dioscorea membranacea Extract |
title_full_unstemmed | Molecular Changes Following Induction of Hepatocellular Carcinoma by Diethylnitrosamine and Thioacetamide, and Subsequent Treatment with Dioscorea membranacea Extract |
title_short | Molecular Changes Following Induction of Hepatocellular Carcinoma by Diethylnitrosamine and Thioacetamide, and Subsequent Treatment with Dioscorea membranacea Extract |
title_sort | molecular changes following induction of hepatocellular carcinoma by diethylnitrosamine and thioacetamide, and subsequent treatment with dioscorea membranacea extract |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608040/ https://www.ncbi.nlm.nih.gov/pubmed/36313224 http://dx.doi.org/10.7150/ijms.72987 |
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