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Multiple proliferation signaling pathways are modulated by octacalcium phosphate in osteoblasts

Octacalcium phosphate (OCP), a type of bioactive ceramics, may be associated with dentine, tooth apatite, and especially bone generation, and promotes wound healing after fracture. Recently, commercial bone grafting products containing a large amount of OCP material have been released because OCP ca...

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Autores principales: Jung, Yoona, Kim, Jooseong, Kim, Sukyoung, Chung, Shin hye, Wie, Jinhong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Ivyspring International Publisher 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608048/
https://www.ncbi.nlm.nih.gov/pubmed/36313230
http://dx.doi.org/10.7150/ijms.77017
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author Jung, Yoona
Kim, Jooseong
Kim, Sukyoung
Chung, Shin hye
Wie, Jinhong
author_facet Jung, Yoona
Kim, Jooseong
Kim, Sukyoung
Chung, Shin hye
Wie, Jinhong
author_sort Jung, Yoona
collection PubMed
description Octacalcium phosphate (OCP), a type of bioactive ceramics, may be associated with dentine, tooth apatite, and especially bone generation, and promotes wound healing after fracture. Recently, commercial bone grafting products containing a large amount of OCP material have been released because OCP can be synthesized in large quantities. It is reported to increase cell proliferation, but the interaction between OCP and cell signaling pathways is still unclear. In this study, first, we demonstrated OCP mediated cell signaling pathways with only purified OCP materials. OCP regulated P38, JNK (c-Jun N-terminal kinase), Src, and AKT (protein kinase B) signaling pathways. OCP crystals appeared in the characteristic ribbon shape but varied by several tens of micrometers in size. The X-ray diffraction pattern was the same as previously reported. We studied two concentrations of OCP (10 mg/ml and 20 mg/ml) to understand whether the effect of OCP on cell signaling pathways is dose dependent. We confirmed that OCP treatment affected cell proliferation and alkaline phosphatase and disrupted Src phosphorylation but did not change the total protein level. P38 phosphorylation was activated with OCP treatment and inhibited by SB203580, but P38 total protein level did not change. OCP inhibited JNK phosphorylation signaling, whereas PD98509 inhibited JNK phosphorylation with or without OCP. Interestingly, the AKT total level decreased after OCP treatment, but AKT phosphorylation increased considerably. Our results demonstrate that OCP materials modulate cell signaling pathways and increase cell proliferation.
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spelling pubmed-96080482022-10-28 Multiple proliferation signaling pathways are modulated by octacalcium phosphate in osteoblasts Jung, Yoona Kim, Jooseong Kim, Sukyoung Chung, Shin hye Wie, Jinhong Int J Med Sci Research Paper Octacalcium phosphate (OCP), a type of bioactive ceramics, may be associated with dentine, tooth apatite, and especially bone generation, and promotes wound healing after fracture. Recently, commercial bone grafting products containing a large amount of OCP material have been released because OCP can be synthesized in large quantities. It is reported to increase cell proliferation, but the interaction between OCP and cell signaling pathways is still unclear. In this study, first, we demonstrated OCP mediated cell signaling pathways with only purified OCP materials. OCP regulated P38, JNK (c-Jun N-terminal kinase), Src, and AKT (protein kinase B) signaling pathways. OCP crystals appeared in the characteristic ribbon shape but varied by several tens of micrometers in size. The X-ray diffraction pattern was the same as previously reported. We studied two concentrations of OCP (10 mg/ml and 20 mg/ml) to understand whether the effect of OCP on cell signaling pathways is dose dependent. We confirmed that OCP treatment affected cell proliferation and alkaline phosphatase and disrupted Src phosphorylation but did not change the total protein level. P38 phosphorylation was activated with OCP treatment and inhibited by SB203580, but P38 total protein level did not change. OCP inhibited JNK phosphorylation signaling, whereas PD98509 inhibited JNK phosphorylation with or without OCP. Interestingly, the AKT total level decreased after OCP treatment, but AKT phosphorylation increased considerably. Our results demonstrate that OCP materials modulate cell signaling pathways and increase cell proliferation. Ivyspring International Publisher 2022-09-25 /pmc/articles/PMC9608048/ /pubmed/36313230 http://dx.doi.org/10.7150/ijms.77017 Text en © The author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/). See http://ivyspring.com/terms for full terms and conditions.
spellingShingle Research Paper
Jung, Yoona
Kim, Jooseong
Kim, Sukyoung
Chung, Shin hye
Wie, Jinhong
Multiple proliferation signaling pathways are modulated by octacalcium phosphate in osteoblasts
title Multiple proliferation signaling pathways are modulated by octacalcium phosphate in osteoblasts
title_full Multiple proliferation signaling pathways are modulated by octacalcium phosphate in osteoblasts
title_fullStr Multiple proliferation signaling pathways are modulated by octacalcium phosphate in osteoblasts
title_full_unstemmed Multiple proliferation signaling pathways are modulated by octacalcium phosphate in osteoblasts
title_short Multiple proliferation signaling pathways are modulated by octacalcium phosphate in osteoblasts
title_sort multiple proliferation signaling pathways are modulated by octacalcium phosphate in osteoblasts
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608048/
https://www.ncbi.nlm.nih.gov/pubmed/36313230
http://dx.doi.org/10.7150/ijms.77017
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