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In-vitro Evaluation for Effects of Intracanal Medicaments on Viability, Proliferation, and Differentiation of Stem Cells From Apical Papilla – A Systematic Review

This systematic review aimed to evaluate the cytotoxicity of intracanal medicaments used for root canal disinfection and assess their effect on the proliferation and differentiation potential of stem cells from apical papilla (SCAP). The PubMed/Medline, Cochrane, Scopus, EMBASE, CINAHL and Web of Sc...

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Autores principales: Rahul, Morankar, Tewari, Nitesh, Mathur, Vijay, Mohammad, Atif, Bansal, Kalpana, Agrawal, Sandeep, Mir, Riyaz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kare Publishing 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608127/
https://www.ncbi.nlm.nih.gov/pubmed/36217639
http://dx.doi.org/10.14744/eej.2022.63835
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author Rahul, Morankar
Tewari, Nitesh
Mathur, Vijay
Mohammad, Atif
Bansal, Kalpana
Agrawal, Sandeep
Mir, Riyaz
author_facet Rahul, Morankar
Tewari, Nitesh
Mathur, Vijay
Mohammad, Atif
Bansal, Kalpana
Agrawal, Sandeep
Mir, Riyaz
author_sort Rahul, Morankar
collection PubMed
description This systematic review aimed to evaluate the cytotoxicity of intracanal medicaments used for root canal disinfection and assess their effect on the proliferation and differentiation potential of stem cells from apical papilla (SCAP). The PubMed/Medline, Cochrane, Scopus, EMBASE, CINAHL and Web of Science databases were searched. Studies evaluating the effect of intracanal medicament on human SCAP (in-vitro primary cell culture experiment) were included in this systematic review. The risk of bias analysis of included studies was carried out using the Toxicological data Reliability Assessment tool. The data was analysed for qualitative characteristics. A meta-analysis was not carried out considering the heterogeneity of selected studies in terms of cell culture experiments, methods of analysis and the interpretation of results. Four studies fulfilled the desired inclusion criteria. The different antibiotic pastes and their intracanal concentrations lead to reduction in the SCAP survival compared to calcium hydroxide medicament. The findings were insufficient to make a clear distinction between different antibiotic pastes regarding their cytotoxicity. Within the limitations of the present systematic review, it can be concluded that calcium hydroxide is a relatively better intracanal medicament than antibiotic paste mixtures in terms of their cytotoxicity and effect on proliferation of SCAP.
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spelling pubmed-96081272022-11-04 In-vitro Evaluation for Effects of Intracanal Medicaments on Viability, Proliferation, and Differentiation of Stem Cells From Apical Papilla – A Systematic Review Rahul, Morankar Tewari, Nitesh Mathur, Vijay Mohammad, Atif Bansal, Kalpana Agrawal, Sandeep Mir, Riyaz Eur Endod J Systematic Review This systematic review aimed to evaluate the cytotoxicity of intracanal medicaments used for root canal disinfection and assess their effect on the proliferation and differentiation potential of stem cells from apical papilla (SCAP). The PubMed/Medline, Cochrane, Scopus, EMBASE, CINAHL and Web of Science databases were searched. Studies evaluating the effect of intracanal medicament on human SCAP (in-vitro primary cell culture experiment) were included in this systematic review. The risk of bias analysis of included studies was carried out using the Toxicological data Reliability Assessment tool. The data was analysed for qualitative characteristics. A meta-analysis was not carried out considering the heterogeneity of selected studies in terms of cell culture experiments, methods of analysis and the interpretation of results. Four studies fulfilled the desired inclusion criteria. The different antibiotic pastes and their intracanal concentrations lead to reduction in the SCAP survival compared to calcium hydroxide medicament. The findings were insufficient to make a clear distinction between different antibiotic pastes regarding their cytotoxicity. Within the limitations of the present systematic review, it can be concluded that calcium hydroxide is a relatively better intracanal medicament than antibiotic paste mixtures in terms of their cytotoxicity and effect on proliferation of SCAP. Kare Publishing 2022-09-12 /pmc/articles/PMC9608127/ /pubmed/36217639 http://dx.doi.org/10.14744/eej.2022.63835 Text en © Copyright 2022 by European Endodontic Journal https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/)
spellingShingle Systematic Review
Rahul, Morankar
Tewari, Nitesh
Mathur, Vijay
Mohammad, Atif
Bansal, Kalpana
Agrawal, Sandeep
Mir, Riyaz
In-vitro Evaluation for Effects of Intracanal Medicaments on Viability, Proliferation, and Differentiation of Stem Cells From Apical Papilla – A Systematic Review
title In-vitro Evaluation for Effects of Intracanal Medicaments on Viability, Proliferation, and Differentiation of Stem Cells From Apical Papilla – A Systematic Review
title_full In-vitro Evaluation for Effects of Intracanal Medicaments on Viability, Proliferation, and Differentiation of Stem Cells From Apical Papilla – A Systematic Review
title_fullStr In-vitro Evaluation for Effects of Intracanal Medicaments on Viability, Proliferation, and Differentiation of Stem Cells From Apical Papilla – A Systematic Review
title_full_unstemmed In-vitro Evaluation for Effects of Intracanal Medicaments on Viability, Proliferation, and Differentiation of Stem Cells From Apical Papilla – A Systematic Review
title_short In-vitro Evaluation for Effects of Intracanal Medicaments on Viability, Proliferation, and Differentiation of Stem Cells From Apical Papilla – A Systematic Review
title_sort in-vitro evaluation for effects of intracanal medicaments on viability, proliferation, and differentiation of stem cells from apical papilla – a systematic review
topic Systematic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608127/
https://www.ncbi.nlm.nih.gov/pubmed/36217639
http://dx.doi.org/10.14744/eej.2022.63835
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