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In-vitro Evaluation for Effects of Intracanal Medicaments on Viability, Proliferation, and Differentiation of Stem Cells From Apical Papilla – A Systematic Review
This systematic review aimed to evaluate the cytotoxicity of intracanal medicaments used for root canal disinfection and assess their effect on the proliferation and differentiation potential of stem cells from apical papilla (SCAP). The PubMed/Medline, Cochrane, Scopus, EMBASE, CINAHL and Web of Sc...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Kare Publishing
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608127/ https://www.ncbi.nlm.nih.gov/pubmed/36217639 http://dx.doi.org/10.14744/eej.2022.63835 |
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author | Rahul, Morankar Tewari, Nitesh Mathur, Vijay Mohammad, Atif Bansal, Kalpana Agrawal, Sandeep Mir, Riyaz |
author_facet | Rahul, Morankar Tewari, Nitesh Mathur, Vijay Mohammad, Atif Bansal, Kalpana Agrawal, Sandeep Mir, Riyaz |
author_sort | Rahul, Morankar |
collection | PubMed |
description | This systematic review aimed to evaluate the cytotoxicity of intracanal medicaments used for root canal disinfection and assess their effect on the proliferation and differentiation potential of stem cells from apical papilla (SCAP). The PubMed/Medline, Cochrane, Scopus, EMBASE, CINAHL and Web of Science databases were searched. Studies evaluating the effect of intracanal medicament on human SCAP (in-vitro primary cell culture experiment) were included in this systematic review. The risk of bias analysis of included studies was carried out using the Toxicological data Reliability Assessment tool. The data was analysed for qualitative characteristics. A meta-analysis was not carried out considering the heterogeneity of selected studies in terms of cell culture experiments, methods of analysis and the interpretation of results. Four studies fulfilled the desired inclusion criteria. The different antibiotic pastes and their intracanal concentrations lead to reduction in the SCAP survival compared to calcium hydroxide medicament. The findings were insufficient to make a clear distinction between different antibiotic pastes regarding their cytotoxicity. Within the limitations of the present systematic review, it can be concluded that calcium hydroxide is a relatively better intracanal medicament than antibiotic paste mixtures in terms of their cytotoxicity and effect on proliferation of SCAP. |
format | Online Article Text |
id | pubmed-9608127 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Kare Publishing |
record_format | MEDLINE/PubMed |
spelling | pubmed-96081272022-11-04 In-vitro Evaluation for Effects of Intracanal Medicaments on Viability, Proliferation, and Differentiation of Stem Cells From Apical Papilla – A Systematic Review Rahul, Morankar Tewari, Nitesh Mathur, Vijay Mohammad, Atif Bansal, Kalpana Agrawal, Sandeep Mir, Riyaz Eur Endod J Systematic Review This systematic review aimed to evaluate the cytotoxicity of intracanal medicaments used for root canal disinfection and assess their effect on the proliferation and differentiation potential of stem cells from apical papilla (SCAP). The PubMed/Medline, Cochrane, Scopus, EMBASE, CINAHL and Web of Science databases were searched. Studies evaluating the effect of intracanal medicament on human SCAP (in-vitro primary cell culture experiment) were included in this systematic review. The risk of bias analysis of included studies was carried out using the Toxicological data Reliability Assessment tool. The data was analysed for qualitative characteristics. A meta-analysis was not carried out considering the heterogeneity of selected studies in terms of cell culture experiments, methods of analysis and the interpretation of results. Four studies fulfilled the desired inclusion criteria. The different antibiotic pastes and their intracanal concentrations lead to reduction in the SCAP survival compared to calcium hydroxide medicament. The findings were insufficient to make a clear distinction between different antibiotic pastes regarding their cytotoxicity. Within the limitations of the present systematic review, it can be concluded that calcium hydroxide is a relatively better intracanal medicament than antibiotic paste mixtures in terms of their cytotoxicity and effect on proliferation of SCAP. Kare Publishing 2022-09-12 /pmc/articles/PMC9608127/ /pubmed/36217639 http://dx.doi.org/10.14744/eej.2022.63835 Text en © Copyright 2022 by European Endodontic Journal https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/) |
spellingShingle | Systematic Review Rahul, Morankar Tewari, Nitesh Mathur, Vijay Mohammad, Atif Bansal, Kalpana Agrawal, Sandeep Mir, Riyaz In-vitro Evaluation for Effects of Intracanal Medicaments on Viability, Proliferation, and Differentiation of Stem Cells From Apical Papilla – A Systematic Review |
title | In-vitro Evaluation for Effects of Intracanal Medicaments on Viability, Proliferation, and Differentiation of Stem Cells From Apical Papilla – A Systematic Review |
title_full | In-vitro Evaluation for Effects of Intracanal Medicaments on Viability, Proliferation, and Differentiation of Stem Cells From Apical Papilla – A Systematic Review |
title_fullStr | In-vitro Evaluation for Effects of Intracanal Medicaments on Viability, Proliferation, and Differentiation of Stem Cells From Apical Papilla – A Systematic Review |
title_full_unstemmed | In-vitro Evaluation for Effects of Intracanal Medicaments on Viability, Proliferation, and Differentiation of Stem Cells From Apical Papilla – A Systematic Review |
title_short | In-vitro Evaluation for Effects of Intracanal Medicaments on Viability, Proliferation, and Differentiation of Stem Cells From Apical Papilla – A Systematic Review |
title_sort | in-vitro evaluation for effects of intracanal medicaments on viability, proliferation, and differentiation of stem cells from apical papilla – a systematic review |
topic | Systematic Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9608127/ https://www.ncbi.nlm.nih.gov/pubmed/36217639 http://dx.doi.org/10.14744/eej.2022.63835 |
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